Construct: sgRNA BRDN0001146405

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCCGCTCTCTGGTTACATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDPK1 (5170)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77453

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 2561820 - PDPK1 NNNGCTCTCTGGTTACATAG NGG 0 1.0 Tier I
2 NC_000003.12 3 49625009 - BSN NNNGCTCTCTGGTTACCCAG NGG 2 0.0756 Tier I
3 NC_000016.10 16 66913528 + CDH16 NNNGCTCTCTGGTCACGTAG NGG 2 0.0504 Tier I
4 NC_000002.12 2 84800130 - DNAH6 NNNGTTCTTTGGTTACATAG NGG 2 0.5568 Tier II
5 NC_000002.12 2 144157111 + GTDC1 NNNGCTCTCTAGTTACATGG NGG 2 0.375 Tier II
6 NC_000003.12 3 77372968 + ROBO2 NNNGCTCTCTGTTTACATAT NGG 2 0.2692 Tier II
7 NC_000003.12 3 13193435 + IQSEC1 NNNGCTCTCTGGCTACAGAG NGG 2 0.2632 Tier II
8 NC_000013.11 13 20144043 + GJA3 NNNGCTCTCTTGTTACAAAG NGG 2 0.2564 Tier II
9 NC_000012.12 12 26145270 - SSPN NNNTCTCTCTGGTTACAAAG NGG 2 0.2424 Tier II
10 NC_000007.14 7 44763794 + ZMIZ2 NNNTCTCTGTGGTTACATAG NGG 2 0.2251 Tier II
11 NC_000007.14 7 102917112 + LRRC17 NNNGCTCTCTGGTTTCACAG NGG 2 0.0857 Tier II
12 NC_000007.14 7 102917112 + FBXL13 NNNGCTCTCTGGTTTCACAG NGG 2 0.0857 Tier II
13 NC_000001.11 1 60033738 - C1orf87 NNNGCTCTCTGGTTCCATAG NGT 2 0.0044 Tier II
14 NC_000016.10 16 2638373 + PDPK2P NNNGCTCTCTGGTTACATAG NGG 0 1.0 Tier III
15 NC_000006.12 6 15970276 - LOC105374949 NNNGTTCTCTGGTTAAATAG NGG 2 0.6364 Tier III
16 NC_000005.10 5 135253746 + PITX1-AS1 NNNGCTCTCAGGTTACATAG NAG 2 0.2222 Tier III
17 NC_000003.12 3 148215147 + LINC02045 NNNGTTCTCTGGTTATATAG NGG 2 0.1958 Tier III
18 NC_000006.12 6 40955755 - LOC101929555 NNNGCTCTCTGGTTACAAAG NGA 2 0.0463 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 104138028 + Sgtb NNNGCTCTTTGATTACATAG NGG 2 0.8167 Tier II
2 NC_000082.6 16 17110258 - Ppil2 NNNGCTCAGTGGTTACATAG NGG 2 0.4952 Tier II
3 NC_000077.6 11 83528162 - Ccl5 NNNGCTCACTGGTTACATGG NGG 2 0.3 Tier II
4 NC_000080.6 14 24170715 - Dlg5 NNNTGTCTCTGGTTACATAG NGG 2 0.2182 Tier II
5 NC_000076.6 10 20559988 + Pde7b NNNTCTCTCTGGTTACATTG NGG 2 0.1958 Tier II
6 NC_000074.6 8 117199714 - Gan NNNGCTCTCTGTTTACACAG NGG 2 0.1648 Tier II
7 NC_000070.6 4 125655915 - Grik3 NNNGCTCTCTGGTTACAAAG NCG 2 0.0714 Tier II
8 NC_000081.6 15 20862983 - Cdh12 NNNGCTCTCTGGTTACATAG NTG 1 0.039 Tier II
9 NC_000069.6 3 103922564 - Rsbn1 NNNGCTCACTGGTTACATAG NTG 2 0.0312 Tier II
10 NC_000072.6 6 53373207 + Creb5 NNNGCTCTCTGGTTTCTTAG NGG 2 0.0267 Tier II
11 NC_000068.7 2 4142475 + Frmd4a NNNGCTCTCTGGTTGCATAG NTG 2 0.0253 Tier II
12 NC_000081.6 15 39034300 - Fzd6 NNNGCCCTCTGGTTACATAG NGT 2 0.0147 Tier II
13 NC_000073.6 7 109320441 - Stk33 NNNGCTCTCTGGTTACAGAG NGT 2 0.0054 Tier II
14 NC_000073.6 7 58752380 + Atp10a NNNGCTCTCTGGTGAAATAG NGG 2 0.0 Tier II
15 NC_000070.6 4 148657081 - Gm572 NNNTCTCTCTGGTGACATAG NGG 2 0.0 Tier II
16 NC_000077.6 11 83528162 - Gm32650 NNNGCTCACTGGTTACATGG NGG 2 0.3 Tier III
17 NC_000070.6 4 54874993 + Gm34133 NNNTTTCTCTGGTTACATAG NGG 2 0.2314 Tier III
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Other clones with same target sequence:

(none)