Construct: sgRNA BRDN0001146407

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGATATTAGATATTGATCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK8 (5599)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78048

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 48425896 + MAPK8 NNNTATTAGATATTGATCAG NGG 0 1.0 Tier I
2 NC_000005.10 5 180242748 - MAPK9 NNNCCTTAGATATTGATCAG NGG 2 0.3235 Tier I
3 NC_000006.12 6 36095995 + MAPK14 NNNCATTAGATATTGATCAG NTG 2 0.0252 Tier I
4 NC_000018.10 18 63546006 - SERPINB12 NNNTATAAGATATTGACCAG NGG 2 0.6176 Tier II
5 NC_000010.11 10 67516742 - CTNNA3 NNNTAGTAGATATTGATCAA NGG 2 0.5357 Tier II
6 NC_000010.11 10 126378563 + ADAM12 NNNTATCAGATATTGATTAG NGG 2 0.442 Tier II
7 NC_000012.12 12 28358721 + CCDC91 NNNTAGTGGATATTGATCAG NGG 2 0.419 Tier II
8 NC_000004.12 4 15824617 + CD38 NNNTATAAGATTTTGATCAG NGG 2 0.2917 Tier II
9 NC_000011.10 11 100938977 - ARHGAP42 NNNTATTTGATATTGATCAC NGG 2 0.1837 Tier II
10 NC_000022.11 22 33083626 + LARGE1 NNNTATCAGATAGTGATCAG NGG 2 0.1793 Tier II
11 NC_000019.10 19 43918756 + ZNF45 NNNGATTAGATATTCATCAG NGG 2 0.1705 Tier II
12 NC_000009.12 9 92365192 + CENPP NNNTATTAGATATCGATAAG NGG 2 0.1538 Tier II
13 NC_000009.12 9 109489125 + PTPN3 NNNTATTAGAAATTGGTCAG NGG 2 0.1442 Tier II
14 NC_000011.10 11 21453345 - NELL1 NNNTATTAGATAATTATCAG NGG 2 0.0989 Tier II
15 NC_000004.12 4 75801399 + USO1 NNNTATTAGATAATGATGAG NGG 2 0.0923 Tier II
16 NC_000004.12 4 121935685 + TRPC3 NNNTATTATATATTGATGAG NGG 2 0.0711 Tier II
17 NC_000010.11 10 10639662 - CELF2 NNNTATTAGATATTGATCAG NGA 1 0.0694 Tier II
18 NC_000002.12 2 188444481 - GULP1 NNNTATTAGATATTGATCAA NGA 2 0.0651 Tier II
19 NC_000003.12 3 61638823 + PTPRG NNNTATTAGATATTGGTCAG NTG 2 0.0075 Tier II
20 NC_000005.10 5 94951356 - MCTP1 NNNTATTAGATATTGGTCAG NTG 2 0.0075 Tier II
21 NC_000014.9 14 54972848 + WDHD1 NNNTATTAGATATTGGTCAG NGT 2 0.0031 Tier II
22 NC_000023.11 X 29639844 + IL1RAPL1 NNNTATTAGATATGCATCAG NGG 2 0.0 Tier II
23 NC_000010.11 10 47967025 + ANXA8 NNNTATTAGATATTGATCAG NTC 2 0.0 Tier II
24 NC_000001.11 1 7337037 - CAMTA1 NNNTATTAGTTATTGCTCAG NGG 2 0.0 Tier II
25 NC_000012.12 12 45461448 + LOC105369743 NNNCATTAGATATTGATCAG NGG 1 0.6471 Tier III
26 NC_000012.12 12 87047133 + LOC105369878 NNNTAATAGATATTGATCAT NGG 2 0.6067 Tier III
27 NC_000001.11 1 98174671 + LOC124900404 NNNTACTAGATATTGAACAG NGG 2 0.4848 Tier III
28 NC_000008.11 8 69426449 - LINC01603 NNNTCTTAGATAATGATCAG NGG 2 0.3462 Tier III
29 NC_000019.10 19 43918756 + ZNF45-AS1 NNNGATTAGATATTCATCAG NGG 2 0.1705 Tier III
30 NC_000011.10 11 80141154 - LOC105369406 NNNTATTAGATATTGATAAG NGA 2 0.0374 Tier III
31 NC_000023.11 X 119256814 + AKAP17BP NNNTATTAGTTATTGATCAG NGC 2 0.0196 Tier III
32 NC_000010.11 10 46417637 - LINC00842 NNNTATTAGATATTGATCAG NTC 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 33388975 - Mapk8 NNNTAATAGATATTGATCAG NGG 1 0.8667 Tier I
2 NC_000067.6 1 9552951 + Adhfe1 NNNTATTAGATATGGAGCAG NGG 2 0.0 Tier I
3 NC_000084.6 18 72000825 + Dcc NNNTATTAGTCATTGATCAG NGG 2 0.5882 Tier II
4 NC_000081.6 15 39238621 - Rims2 NNNTATTACATATTGATTAG NGG 2 0.3462 Tier II
5 NC_000072.6 6 47297661 - Cntnap2 NNNTTTTAGATATTGATCAT NGG 2 0.2545 Tier II
6 NC_000084.6 18 71990570 - Dcc NNNTATAAGATAGTGATCAG NGG 2 0.2283 Tier II
7 NC_000067.6 1 182017069 - Enah NNNTATTAGATCTTGATTAG NGG 2 0.1692 Tier II
8 NC_000067.6 1 184820693 + Mtarc2 NNNTATTAGAAATTGATGAG NGG 2 0.1 Tier II
9 NC_000073.6 7 43800958 + Klk8 NNNTATTAGATATTGAGCTG NGG 2 0.0633 Tier II
10 NC_000071.6 5 14593060 + Pclo NNNTATTAGATATTGATCAA NGT 2 0.0151 Tier II
11 NC_000077.6 11 47767430 - Sgcd NNNTATTAGGTATTGATCAG NGT 2 0.009 Tier II
12 NC_000086.7 X 51145171 - Mbnl3 NNNGATTAGATATGGATCAG NGG 2 0.0 Tier II
13 NC_000083.6 17 43156825 + E130008D07Rik NNNTATTAGATATTGACCAA NGG 2 0.6618 Tier III
14 NC_000076.6 10 70624623 + D630013N20Rik NNNTATGAGTTATTGATCAG NGG 2 0.519 Tier III
15 NC_000072.6 6 48872964 + Gm44764 NNNTACTAGATATGGATCAG NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)