Construct: sgRNA BRDN0001146409

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCCAGGAACTCTGTGACAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAD (790)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76963

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 27226844 + CAD NNNAGGAACTCTGTGACAGG NGG 0 1.0 Tier I
2 NC_000011.10 11 45226687 - PRDM11 NNNAGGAACTCTGTGGCCGG NGG 2 0.0366 Tier I
3 NC_000001.11 1 24128232 + IL22RA1 NNNAGGAACTCTGTGTCAGG NGT 2 0.0 Tier I
4 NC_000022.11 22 19268185 - CLTCL1 NNNAGGAACACTGTGACAGA NGG 2 0.8036 Tier II
5 NC_000016.10 16 85732333 - C16orf74 NNNAGGAACTCTGTAACATG NGG 2 0.6275 Tier II
6 NC_000004.12 4 176157965 + WDR17 NNNAGGAACTCTGTGAAAGG NGG 1 0.4667 Tier II
7 NC_000006.12 6 36547337 + STK38 NNNAGGGACTCTGAGACAGG NGG 2 0.437 Tier II
8 NC_000001.11 1 94457626 - ABCD3 NNNAGGAAGTCTGTGACATG NGG 2 0.4127 Tier II
9 NC_000020.11 20 42761948 + PTPRT NNNAGCAACTCTGTGAAAGG NGG 2 0.3182 Tier II
10 NC_000022.11 22 38729311 - GTPBP1 NNNAGGAAGGCTGTGACAGG NGG 2 0.3095 Tier II
11 NC_000016.10 16 27327192 - IL4R NNNAGCACCTCTGTGACAGG NGG 2 0.2922 Tier II
12 NC_000008.11 8 129711161 + GSDMC NNNAGTTACTCTGTGACAGG NGG 2 0.2917 Tier II
13 NC_000002.12 2 128283599 + HS6ST1 NNNTGGAACTCTGTGACAGC NGG 2 0.2727 Tier II
14 NC_000002.12 2 40319637 - SLC8A1 NNNAGGAGCTTTGTGACAGG NGG 2 0.2256 Tier II
15 NC_000009.12 9 89421158 + SEMA4D NNNAGGAACACTGTGACAGG NAG 2 0.2222 Tier II
16 NC_000003.12 3 126491685 + UROC1 NNNAAGAACTCTGTGGCAGG NGG 2 0.1667 Tier II
17 NC_000008.11 8 60517526 + RAB2A NNNAGGAAGTGTGTGACAGG NGG 2 0.1548 Tier II
18 NC_000003.12 3 4833613 - ITPR1 NNNAGGAACTCTGTGAAAGG NCG 2 0.05 Tier II
19 NC_000016.10 16 5402189 + RBFOX1 NNNAGGGACTCTGTGACAGG NTG 2 0.0275 Tier II
20 NC_000005.10 5 11310213 - CTNND2 NNNAGGAACTCTGTGAGAGG NTG 2 0.0023 Tier II
21 NC_000005.10 5 146042685 + SH3RF2 NNNAGCAACTCTGTGCCAGG NGG 2 0.0 Tier II
22 NC_000015.10 15 75922926 + FBXO22 NNNAGGAACACTGGGACAGG NGG 2 0.0 Tier II
23 NC_000004.12 4 37642564 - RELL1 NNNAGGAACTCTGTGACAGG NAC 2 0.0 Tier II
24 NC_000006.12 6 85455172 - NT5E NNNAGGAACTCTGTGACAGG NAC 2 0.0 Tier II
25 NC_000002.12 2 64125405 + PELI1 NNNAGGAACTCTGTGCCAGG NAG 2 0.0 Tier II
26 NC_000007.14 7 90927461 + CDK14 NNNAGGAACTCTGTGCCAGG NTG 2 0.0 Tier II
27 NC_000016.10 16 89467071 + ANKRD11 NNNAGGAACTCTGTGCCAGG NGT 2 0.0 Tier II
28 NC_000001.11 1 205400608 - LEMD1 NNNAGGAGCTCTGTGCCAGG NGG 2 0.0 Tier II
29 NC_000020.11 20 47392360 - LINC01754 NNNAAGAACTCTGTAACAGG NGG 2 0.8157 Tier III
30 NC_000011.10 11 27167797 - BBOX1-AS1 NNNAGGAACTCTGTGACAAG NAG 2 0.1852 Tier III
31 NC_000020.11 20 17191215 + LOC105372544 NNNAGGAACTGTGTGACAGG NAG 2 0.0648 Tier III
32 NC_000003.12 3 4833613 - LOC124906209 NNNAGGAACTCTGTGAAAGG NCG 2 0.05 Tier III
33 NC_000008.11 8 116055170 - LINC00536 NNNAGGAACTCTTTGACAGG NGA 2 0.0208 Tier III
34 NC_000005.10 5 152351361 - LOC105378237 NNNAGGCACTCTGTGACAGG NGC 2 0.0097 Tier III
35 NC_000005.10 5 146042685 + LOC107986458 NNNAGCAACTCTGTGCCAGG NGG 2 0.0 Tier III
36 NC_000005.10 5 146042685 + LOC107986459 NNNAGCAACTCTGTGCCAGG NGG 2 0.0 Tier III
37 NC_000001.11 1 54943498 - LOC124904184 NNNAGGGACTCTGTGCCAGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 51511171 - Ano5 NNNAGGACCTCTGTGACAGG NGG 1 0.4286 Tier I
2 NC_000080.6 14 54961091 + Myh6 NNNAGGCACTCTGTGACAGA NGG 2 0.4102 Tier I
3 NC_000067.6 1 34502020 - Prss39 NNNAGGAACTCTGTGGGAGG NGG 2 0.0113 Tier I
4 NC_000079.6 13 38020237 - Cage1 NNNAGAAACTCTGTGACATG NGG 2 0.6667 Tier II
5 NC_000069.6 3 95216975 + Gabpb2 NNNAGGAAATCTGTGACAAG NGG 2 0.6122 Tier II
6 NC_000070.6 4 104958411 + Fyb2 NNNGGGAACTCTGTGACAAG NGG 2 0.4464 Tier II
7 NC_000086.7 X 99826079 - Tmem28 NNNAGGAAAGCTGTGACAGG NGG 2 0.4286 Tier II
8 NC_000073.6 7 64902915 + Fam189a1 NNNAGGAACACTGTGAAAGG NGG 2 0.4 Tier II
9 NC_000071.6 5 140762491 + Gna12 NNNAGGAACTATGTGACAGC NGG 2 0.3214 Tier II
10 NC_000068.7 2 26921627 + Surf4 NNNAGGAACTCGGAGACAGG NGG 2 0.3095 Tier II
11 NC_000070.6 4 149545233 + Ctnnbip1 NNNAGGAGCTCTCTGACAGG NGG 2 0.3088 Tier II
12 NC_000068.7 2 165065987 - Cd40 NNNAGGAACAGTGTGACAGG NGG 2 0.2143 Tier II
13 NC_000068.7 2 120713105 + Stard9 NNNAGGAACTCTTTGACAAG NGG 2 0.2143 Tier II
14 NC_000073.6 7 68161538 + Igf1r NNNAGGACCTCTGTGACGGG NGG 2 0.1714 Tier II
15 NC_000076.6 10 68266437 - Arid5b NNNAGGAACTCTGAGACAGG NAG 2 0.1605 Tier II
16 NC_000077.6 11 8658377 - Tns3 NNNAGGAAGTCTGTGACAGG NAG 2 0.1605 Tier II
17 NC_000087.7 Y 1045960 - Eif2s3y NNNAGGCACTGTGTGACAGG NGG 2 0.1094 Tier II
18 NC_000082.6 16 88244408 + Grik1 NNNAAGAACTCTGTGAGAGG NGG 2 0.051 Tier II
19 NC_000076.6 10 88283684 + Washc3 NNNAGGAACTATGTGAGAGG NGG 2 0.0441 Tier II
20 NC_000073.6 7 126266317 - Sbk1 NNNAGGAACTCTGAGACAGG NGA 2 0.043 Tier II
21 NC_000079.6 13 35719679 + Cdyl NNNTGGAACTCTGTGAGAGG NGG 2 0.0374 Tier II
22 NC_000084.6 18 31178159 + Rit2 NNNAGGAACTCTGTGAGGGG NGG 2 0.0235 Tier II
23 NC_000068.7 2 79894095 + Pde1a NNNAGGAACTGTGTGAGAGG NGG 2 0.0147 Tier II
24 NC_000073.6 7 101723348 - Clpb NNNAAGAACTCTGTGACAGG NGT 2 0.014 Tier II
25 NC_000075.6 9 42641428 + Grik4 NNNAAGAACTCTGTGACAGG NGT 2 0.014 Tier II
26 NC_000070.6 4 59335820 - Susd1 NNNAGGAACTCTGTGGGAGG NGG 2 0.0113 Tier II
27 NC_000075.6 9 123012532 + Kif15 NNNAGGAACTCTGTCACAGG NTG 2 0.0106 Tier II
28 NC_000069.6 3 27545829 + Fndc3b NNNAGGAACTCTGGGAAAGG NGG 2 0.0 Tier II
29 NC_000086.7 X 92489367 + Mageb18 NNNAGGAACTCTGGGACAGG NGA 2 0.0 Tier II
30 NC_000082.6 16 23609203 + Rtp4 NNNAGGAACTCTGGGACGGG NGG 2 0.0 Tier II
31 NC_000073.6 7 130674426 + Tacc2 NNNAGGAACTCTGGGACTGG NGG 2 0.0 Tier II
32 NC_000076.6 10 26787151 + Arhgap18 NNNAGGAACTCTGGGAGAGG NGG 2 0.0 Tier II
33 NC_000074.6 8 34814640 + Dusp4 NNNAGGAACTCTGTGCCAGG NGA 2 0.0 Tier II
34 NC_000078.6 12 116725799 + Ptprn2 NNNAGGATCTCTGGGACAGG NGG 2 0.0 Tier II
35 NC_000084.6 18 38276699 - Pcdh12 NNNGGGAACTCTGGGACAGG NGG 2 0.0 Tier II
36 NC_000079.6 13 69992801 - Gm36019 NNNAGAAACTCTGTGACAGG NAG 2 0.2593 Tier III
37 NC_000077.6 11 86260482 + Brip1os NNNAGGATCTCTGTGACAGG NAG 2 0.1111 Tier III
38 NC_000085.6 19 26849731 - Gm46648 NNNAGGAACTCTGTAACAGG NGA 2 0.0654 Tier III
39 NC_000078.6 12 114999573 - Igh NNNAGAAACTCTGTGACAGG NGC 2 0.0222 Tier III
40 NC_000072.6 6 31619008 - Gm31486 NNNAGGAACTCTGTGACAGG NGC 1 0.0222 Tier III
41 NC_000076.6 10 70902521 - 4930533K18Rik NNNAGGAACTCTGGGAAAGG NGG 2 0.0 Tier III
42 NC_000083.6 17 14239019 - Gm41531 NNNAGGAACTCTGGGACAGC NGG 2 0.0 Tier III
43 NC_000083.6 17 83213982 - Gm34686 NNNAGGAACTCTGTGACAGG NAA 2 0.0 Tier III
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Other clones with same target sequence:

(none)