Construct: sgRNA BRDN0001146410

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAGCAGAGATGTCAAGCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADK (132)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75743

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 74200798 - ADK NNNGCAGAGATGTCAAGCAG NGG 0 1.0 Tier I
2 NC_000008.11 8 60562102 - RAB2A NNNGCAGAGATGTCAAGCAA NGG 1 0.9375 Tier II
3 NC_000016.10 16 23207446 - SCNN1G NNNGCAAAGATGCCAAGCAG NGG 2 0.7895 Tier II
4 NC_000010.11 10 54619593 + PCDH15 NNNGCAGAGATGTTAAGCAG NGG 1 0.7333 Tier II
5 NC_000015.10 15 43531039 - MAP1A NNNGCAGAGAAGCCAAGCAG NGG 2 0.5921 Tier II
6 NC_000002.12 2 55300296 + CCDC88A NNNGCAGAGATGTCAAGAAG NGG 1 0.5385 Tier II
7 NC_000015.10 15 87958431 - NTRK3 NNNGCAGAGATGCCAAGTAG NGG 2 0.5075 Tier II
8 NC_000017.11 17 63782425 - DDX42 NNNGTAGAGATGCCAAGCAG NGG 2 0.5024 Tier II
9 NC_000017.11 17 2875908 - RAP1GAP2 NNNGCAGAGAAGTCAAGCTG NGG 2 0.4038 Tier II
10 NC_000002.12 2 143204883 - ARHGAP15 NNNGCAGAGATGTAAAGCAG NGG 1 0.35 Tier II
11 NC_000007.14 7 23309816 - MALSU1 NNNGCAGAGAAGTCAAGCAC NGG 2 0.3214 Tier II
12 NC_000018.10 18 61847828 - RNF152 NNNGCAGAGATTCCAAGCAG NGG 2 0.3036 Tier II
13 NC_000020.11 20 33544216 - CBFA2T2 NNNGCTGAGAGGTCAAGCAG NGG 2 0.2857 Tier II
14 NC_000017.11 17 56809278 - C17orf67 NNNGCAGGGATTTCAAGCAG NGG 2 0.2821 Tier II
15 NC_000012.12 12 130475917 + RIMBP2 NNNGCAGTGGTGTCAAGCAG NGG 2 0.2381 Tier II
16 NC_000001.11 1 11523598 + DISP3 NNNACAGAGATGGCAAGCAG NGG 2 0.2348 Tier II
17 NC_000002.12 2 36775083 - VIT NNNGCAGAGATGGTAAGCAG NGG 2 0.1913 Tier II
18 NC_000007.14 7 137724765 + DGKI NNNGCAGAGATGTCAAGTAG NAG 2 0.1667 Tier II
19 NC_000011.10 11 64093450 - FLRT1 NNNGCAGAGATGCCAGGCAG NGG 2 0.1518 Tier II
20 NC_000011.10 11 64093450 - MACROD1 NNNGCAGAGATGCCAGGCAG NGG 2 0.1518 Tier II
21 NC_000003.12 3 133935954 + SLCO2A1 NNNGCAGAGGTGTCCAGCAG NGG 2 0.1515 Tier II
22 NC_000013.11 13 24178263 - SPATA13 NNNGCAGAGGTGGCAAGCAG NGG 2 0.1449 Tier II
23 NC_000006.12 6 29461222 - OR2H1 NNNGCAGAGGTGTCAATCAG NGG 2 0.1389 Tier II
24 NC_000014.9 14 78257976 + NRXN3 NNNGCAGAGATATCAAGGAG NGG 2 0.1244 Tier II
25 NC_000016.10 16 17356584 + XYLT1 NNNGCAGAGATGCCAAGGAG NGG 2 0.1053 Tier II
26 NC_000002.12 2 38590284 - HNRNPLL NNNTCAGAGATGTCCAGCAG NGG 2 0.0992 Tier II
27 NC_000012.12 12 113176773 - DDX54 NNNGCAGAGAGGTCAACCAG NGG 2 0.0941 Tier II
28 NC_000004.12 4 61868487 - ADGRL3 NNNACAGAGATGTCAAGCAG NGA 2 0.0625 Tier II
29 NC_000014.9 14 90933273 - RPS6KA5 NNNGCAGAGATGTTAAGCAG NGA 2 0.0509 Tier II
30 NC_000010.11 10 18412587 - CACNB2 NNNGCAGAGATGTCAAGGGG NGG 2 0.05 Tier II
31 NC_000010.11 10 27688032 - MKX NNNGCAGAGAAGTCAAGCAG NTG 2 0.0292 Tier II
32 NC_000003.12 3 44905408 - TGM4 NNNGCAGAGATGTCAGGGAG NGG 2 0.0256 Tier II
33 NC_000006.12 6 5525147 - FARS2 NNNGCAGAGATGGGAAGCAG NGG 2 0.0 Tier II
34 NC_000023.11 X 20193376 + RPS6KA3 NNNGCAGAGATGTGAAGCAC NGG 2 0.0 Tier II
35 NC_000001.11 1 228088243 + ARF1 NNNGCAGAGATGTGAAGGAG NGG 2 0.0 Tier II
36 NC_000010.11 10 133362852 + ECHS1 NNNGCAGAGGTGTGAAGCAG NGG 2 0.0 Tier II
37 NC_000010.11 10 54619593 + LOC105378311 NNNGCAGAGATGTTAAGCAG NGG 1 0.7333 Tier III
38 NC_000004.12 4 143952170 - LOC101927636 NNNACAGAGATCTCAAGCAG NGG 2 0.4765 Tier III
39 NC_000004.12 4 143952170 - LOC105377459 NNNACAGAGATCTCAAGCAG NGG 2 0.4765 Tier III
40 NC_000013.11 13 54838555 + LOC105370213 NNNGGAGAGATGTCAAGAAG NGG 2 0.3231 Tier III
41 NC_000018.10 18 47280328 + LOC124904295 NNNGCAGAGATTCCAAGCAG NGG 2 0.3036 Tier III
42 NC_000012.12 12 42073792 + LOC101929308 NNNGCAGAGATGTCAAGCAG NAG 1 0.2593 Tier III
43 NC_000002.12 2 36775083 - LOC124905990 NNNGCAGAGATGGTAAGCAG NGG 2 0.1913 Tier III
44 NC_000019.10 19 29325911 - VSTM2B-DT NNNGCAGAGCTGTCAATCAG NGG 2 0.0833 Tier III
45 NC_000001.11 1 18051673 - LOC105376812 NNNGCAGAGATTTCAGGCAG NGG 2 0.074 Tier III
46 NC_000001.11 1 34503492 - LOC105378641 NNNGCAGAGATGTCCATCAG NGG 2 0.0682 Tier III
47 NC_000024.10 Y 6453636 - TTTY7 NNNGCAGAAATGTCAAGCAG NGC 2 0.0143 Tier III
48 NC_000024.10 Y 9711095 + TTTY7B NNNGCAGAAATGTCAAGCAG NGC 2 0.0143 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 21092401 - Adk NNNGCAGAGATGTCAAGAAG NGG 1 0.5385 Tier I
2 NC_000068.7 2 121308863 - Map1a NNNGCAGAGAAGCCAAGCAG NGG 2 0.5921 Tier II
3 NC_000075.6 9 54323759 + Gldn NNNGGAGAGATGTCAAACAG NGG 2 0.56 Tier II
4 NC_000080.6 14 33333306 - Arhgap22 NNNGCAGAAATGCCAAGCAG NGG 2 0.5075 Tier II
5 NC_000079.6 13 92030467 + Rasgrf2 NNNGCGCAGATGTCAAGCAG NGG 2 0.4911 Tier II
6 NC_000071.6 5 4071330 - Akap9 NNNGCAGAGGTGACAAGCAG NGG 2 0.3846 Tier II
7 NC_000077.6 11 75214910 + Rtn4rl1 NNNGCAGAGATTTCAAGCAA NGG 2 0.3606 Tier II
8 NC_000081.6 15 73825064 + Mroh5 NNNGCAGAGTTTTCAAGCAG NGG 2 0.3394 Tier II
9 NC_000073.6 7 65129042 + Fam189a1 NNNGGAGAGATGTCAAGAAG NGG 2 0.3231 Tier II
10 NC_000080.6 14 67924994 + Dock5 NNNGGAGAGATGTCAAGAAG NGG 2 0.3231 Tier II
11 NC_000071.6 5 15942589 + Cacna2d1 NNNGCAGAGAGGCCAAGCAG NGG 2 0.3158 Tier II
12 NC_000068.7 2 62398148 - Dpp4 NNNGCAGAGATGGCAAACAG NGG 2 0.2435 Tier II
13 NC_000080.6 14 10427182 - Fhit NNNGCAGAGATGAAAAGCAG NGG 2 0.2423 Tier II
14 NC_000083.6 17 51004448 - Tbc1d5 NNNGCAGAGTTGTCAAGCAG NAG 2 0.2288 Tier II
15 NC_000076.6 10 50782034 - Ascc3 NNNGCAGAGATGCCCAGCAG NGG 2 0.2153 Tier II
16 NC_000080.6 14 93623105 - Pcdh9 NNNGCAGAGATCTAAAGCAG NGG 2 0.1853 Tier II
17 NC_000075.6 9 29381293 - Ntm NNNGCAGAGTTGTCAGGCAG NGG 2 0.1697 Tier II
18 NC_000084.6 18 75993585 - Zbtb7c NNNGCAGAGATGCCAGGCAG NGG 2 0.1518 Tier II
19 NC_000073.6 7 6383778 + Zfp28 NNNGCAGAGAAGTCAGGCAG NGG 2 0.1442 Tier II
20 NC_000075.6 9 108839140 - Celsr3 NNNGCAGAGATCTCAAGCAG NAG 2 0.1373 Tier II
21 NC_000078.6 12 18962262 - Gm40847 NNNGCAGTGATGTCAAGCAG NAG 2 0.1111 Tier II
22 NC_000070.6 4 141202688 - Fbxo42 NNNGCAGAGATCTCAGGCAG NGG 2 0.1018 Tier II
23 NC_000079.6 13 95739381 - Iqgap2 NNNTCAGAGATGTCAACCAG NGG 2 0.0856 Tier II
24 NC_000067.6 1 105298788 + Rnf152 NNNGCAGTGATGTCAGGCAG NGG 2 0.0824 Tier II
25 NC_000069.6 3 22154237 - Tbl1xr1 NNNGCAGAGATGTCAATCAG NAG 2 0.0648 Tier II
26 NC_000078.6 12 81976537 - Pcnx NNNGTAGAGATGTCAAGCAG NGA 2 0.0442 Tier II
27 NC_000079.6 13 37797481 - Rreb1 NNNGCAGAGATGTCAAGCTG NTG 2 0.021 Tier II
28 NC_000079.6 13 97129231 + Fam169a NNNGCAGAGATTTCAAGCAG NTG 2 0.015 Tier II
29 NC_000074.6 8 111490246 + Wdr59 NNNGCAGAGATGTCCAGCAG NGC 2 0.0061 Tier II
30 NC_000068.7 2 20853639 - Arhgap21 NNNGCAGAGATGTGAAGCTG NGG 2 0.0 Tier II
31 NC_000069.6 3 139008224 - Rap1gds1 NNNGCAGAGATGTGAGGCAG NGG 2 0.0 Tier II
32 NC_000081.6 15 13154228 + Cdh6 NNNGCAGAGATTTGAAGCAG NGG 2 0.0 Tier II
33 NC_000070.6 4 136335164 + Hnrnpr NNNTCAGAGATGTGAAGCAG NGG 2 0.0 Tier II
34 NC_000084.6 18 68610087 - Gm30821 NNNGCAGGGATGTCAAACAG NGG 2 0.6844 Tier III
35 NC_000075.6 9 90476596 - Gm39398 NNNGCAGACATGACAAGCAG NGG 2 0.3728 Tier III
36 NC_000069.6 3 64723792 + Vmn2r-ps11 NNNGCAGAGCTGCCAAGCAG NGG 2 0.2632 Tier III
37 NC_000067.6 1 120717335 + 2610027F03Rik NNNGCAGGGATGTCAAGCAG NAG 2 0.1901 Tier III
38 NC_000079.6 13 53477862 + Gm33763 NNNGTAGAGATGTCAACCAG NGG 2 0.1497 Tier III
39 NC_000079.6 13 37797481 - Gm46411 NNNGCAGAGATGTCAAGCTG NTG 2 0.021 Tier III
40 NC_000072.6 6 69979037 + Igk NNNGCAGACATGTGAAGCAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)