Construct: sgRNA BRDN0001146410
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAGCAGAGATGTCAAGCAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ADK (132)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000010.11 | 10 | 74200798 | - | ADK | NNNGCAGAGATGTCAAGCAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000008.11 | 8 | 60562102 | - | RAB2A | NNNGCAGAGATGTCAAGCAA | NGG | 1 | 0.9375 | Tier II |
3 | NC_000016.10 | 16 | 23207446 | - | SCNN1G | NNNGCAAAGATGCCAAGCAG | NGG | 2 | 0.7895 | Tier II |
4 | NC_000010.11 | 10 | 54619593 | + | PCDH15 | NNNGCAGAGATGTTAAGCAG | NGG | 1 | 0.7333 | Tier II |
5 | NC_000015.10 | 15 | 43531039 | - | MAP1A | NNNGCAGAGAAGCCAAGCAG | NGG | 2 | 0.5921 | Tier II |
6 | NC_000002.12 | 2 | 55300296 | + | CCDC88A | NNNGCAGAGATGTCAAGAAG | NGG | 1 | 0.5385 | Tier II |
7 | NC_000015.10 | 15 | 87958431 | - | NTRK3 | NNNGCAGAGATGCCAAGTAG | NGG | 2 | 0.5075 | Tier II |
8 | NC_000017.11 | 17 | 63782425 | - | DDX42 | NNNGTAGAGATGCCAAGCAG | NGG | 2 | 0.5024 | Tier II |
9 | NC_000017.11 | 17 | 2875908 | - | RAP1GAP2 | NNNGCAGAGAAGTCAAGCTG | NGG | 2 | 0.4038 | Tier II |
10 | NC_000002.12 | 2 | 143204883 | - | ARHGAP15 | NNNGCAGAGATGTAAAGCAG | NGG | 1 | 0.35 | Tier II |
11 | NC_000007.14 | 7 | 23309816 | - | MALSU1 | NNNGCAGAGAAGTCAAGCAC | NGG | 2 | 0.3214 | Tier II |
12 | NC_000018.10 | 18 | 61847828 | - | RNF152 | NNNGCAGAGATTCCAAGCAG | NGG | 2 | 0.3036 | Tier II |
13 | NC_000020.11 | 20 | 33544216 | - | CBFA2T2 | NNNGCTGAGAGGTCAAGCAG | NGG | 2 | 0.2857 | Tier II |
14 | NC_000017.11 | 17 | 56809278 | - | C17orf67 | NNNGCAGGGATTTCAAGCAG | NGG | 2 | 0.2821 | Tier II |
15 | NC_000012.12 | 12 | 130475917 | + | RIMBP2 | NNNGCAGTGGTGTCAAGCAG | NGG | 2 | 0.2381 | Tier II |
16 | NC_000001.11 | 1 | 11523598 | + | DISP3 | NNNACAGAGATGGCAAGCAG | NGG | 2 | 0.2348 | Tier II |
17 | NC_000002.12 | 2 | 36775083 | - | VIT | NNNGCAGAGATGGTAAGCAG | NGG | 2 | 0.1913 | Tier II |
18 | NC_000007.14 | 7 | 137724765 | + | DGKI | NNNGCAGAGATGTCAAGTAG | NAG | 2 | 0.1667 | Tier II |
19 | NC_000011.10 | 11 | 64093450 | - | FLRT1 | NNNGCAGAGATGCCAGGCAG | NGG | 2 | 0.1518 | Tier II |
20 | NC_000011.10 | 11 | 64093450 | - | MACROD1 | NNNGCAGAGATGCCAGGCAG | NGG | 2 | 0.1518 | Tier II |
21 | NC_000003.12 | 3 | 133935954 | + | SLCO2A1 | NNNGCAGAGGTGTCCAGCAG | NGG | 2 | 0.1515 | Tier II |
22 | NC_000013.11 | 13 | 24178263 | - | SPATA13 | NNNGCAGAGGTGGCAAGCAG | NGG | 2 | 0.1449 | Tier II |
23 | NC_000006.12 | 6 | 29461222 | - | OR2H1 | NNNGCAGAGGTGTCAATCAG | NGG | 2 | 0.1389 | Tier II |
24 | NC_000014.9 | 14 | 78257976 | + | NRXN3 | NNNGCAGAGATATCAAGGAG | NGG | 2 | 0.1244 | Tier II |
25 | NC_000016.10 | 16 | 17356584 | + | XYLT1 | NNNGCAGAGATGCCAAGGAG | NGG | 2 | 0.1053 | Tier II |
26 | NC_000002.12 | 2 | 38590284 | - | HNRNPLL | NNNTCAGAGATGTCCAGCAG | NGG | 2 | 0.0992 | Tier II |
27 | NC_000012.12 | 12 | 113176773 | - | DDX54 | NNNGCAGAGAGGTCAACCAG | NGG | 2 | 0.0941 | Tier II |
28 | NC_000004.12 | 4 | 61868487 | - | ADGRL3 | NNNACAGAGATGTCAAGCAG | NGA | 2 | 0.0625 | Tier II |
29 | NC_000014.9 | 14 | 90933273 | - | RPS6KA5 | NNNGCAGAGATGTTAAGCAG | NGA | 2 | 0.0509 | Tier II |
30 | NC_000010.11 | 10 | 18412587 | - | CACNB2 | NNNGCAGAGATGTCAAGGGG | NGG | 2 | 0.05 | Tier II |
31 | NC_000010.11 | 10 | 27688032 | - | MKX | NNNGCAGAGAAGTCAAGCAG | NTG | 2 | 0.0292 | Tier II |
32 | NC_000003.12 | 3 | 44905408 | - | TGM4 | NNNGCAGAGATGTCAGGGAG | NGG | 2 | 0.0256 | Tier II |
33 | NC_000006.12 | 6 | 5525147 | - | FARS2 | NNNGCAGAGATGGGAAGCAG | NGG | 2 | 0.0 | Tier II |
34 | NC_000023.11 | X | 20193376 | + | RPS6KA3 | NNNGCAGAGATGTGAAGCAC | NGG | 2 | 0.0 | Tier II |
35 | NC_000001.11 | 1 | 228088243 | + | ARF1 | NNNGCAGAGATGTGAAGGAG | NGG | 2 | 0.0 | Tier II |
36 | NC_000010.11 | 10 | 133362852 | + | ECHS1 | NNNGCAGAGGTGTGAAGCAG | NGG | 2 | 0.0 | Tier II |
37 | NC_000010.11 | 10 | 54619593 | + | LOC105378311 | NNNGCAGAGATGTTAAGCAG | NGG | 1 | 0.7333 | Tier III |
38 | NC_000004.12 | 4 | 143952170 | - | LOC101927636 | NNNACAGAGATCTCAAGCAG | NGG | 2 | 0.4765 | Tier III |
39 | NC_000004.12 | 4 | 143952170 | - | LOC105377459 | NNNACAGAGATCTCAAGCAG | NGG | 2 | 0.4765 | Tier III |
40 | NC_000013.11 | 13 | 54838555 | + | LOC105370213 | NNNGGAGAGATGTCAAGAAG | NGG | 2 | 0.3231 | Tier III |
41 | NC_000018.10 | 18 | 47280328 | + | LOC124904295 | NNNGCAGAGATTCCAAGCAG | NGG | 2 | 0.3036 | Tier III |
42 | NC_000012.12 | 12 | 42073792 | + | LOC101929308 | NNNGCAGAGATGTCAAGCAG | NAG | 1 | 0.2593 | Tier III |
43 | NC_000002.12 | 2 | 36775083 | - | LOC124905990 | NNNGCAGAGATGGTAAGCAG | NGG | 2 | 0.1913 | Tier III |
44 | NC_000019.10 | 19 | 29325911 | - | VSTM2B-DT | NNNGCAGAGCTGTCAATCAG | NGG | 2 | 0.0833 | Tier III |
45 | NC_000001.11 | 1 | 18051673 | - | LOC105376812 | NNNGCAGAGATTTCAGGCAG | NGG | 2 | 0.074 | Tier III |
46 | NC_000001.11 | 1 | 34503492 | - | LOC105378641 | NNNGCAGAGATGTCCATCAG | NGG | 2 | 0.0682 | Tier III |
47 | NC_000024.10 | Y | 6453636 | - | TTTY7 | NNNGCAGAAATGTCAAGCAG | NGC | 2 | 0.0143 | Tier III |
48 | NC_000024.10 | Y | 9711095 | + | TTTY7B | NNNGCAGAAATGTCAAGCAG | NGC | 2 | 0.0143 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000080.6 | 14 | 21092401 | - | Adk | NNNGCAGAGATGTCAAGAAG | NGG | 1 | 0.5385 | Tier I |
2 | NC_000068.7 | 2 | 121308863 | - | Map1a | NNNGCAGAGAAGCCAAGCAG | NGG | 2 | 0.5921 | Tier II |
3 | NC_000075.6 | 9 | 54323759 | + | Gldn | NNNGGAGAGATGTCAAACAG | NGG | 2 | 0.56 | Tier II |
4 | NC_000080.6 | 14 | 33333306 | - | Arhgap22 | NNNGCAGAAATGCCAAGCAG | NGG | 2 | 0.5075 | Tier II |
5 | NC_000079.6 | 13 | 92030467 | + | Rasgrf2 | NNNGCGCAGATGTCAAGCAG | NGG | 2 | 0.4911 | Tier II |
6 | NC_000071.6 | 5 | 4071330 | - | Akap9 | NNNGCAGAGGTGACAAGCAG | NGG | 2 | 0.3846 | Tier II |
7 | NC_000077.6 | 11 | 75214910 | + | Rtn4rl1 | NNNGCAGAGATTTCAAGCAA | NGG | 2 | 0.3606 | Tier II |
8 | NC_000081.6 | 15 | 73825064 | + | Mroh5 | NNNGCAGAGTTTTCAAGCAG | NGG | 2 | 0.3394 | Tier II |
9 | NC_000073.6 | 7 | 65129042 | + | Fam189a1 | NNNGGAGAGATGTCAAGAAG | NGG | 2 | 0.3231 | Tier II |
10 | NC_000080.6 | 14 | 67924994 | + | Dock5 | NNNGGAGAGATGTCAAGAAG | NGG | 2 | 0.3231 | Tier II |
11 | NC_000071.6 | 5 | 15942589 | + | Cacna2d1 | NNNGCAGAGAGGCCAAGCAG | NGG | 2 | 0.3158 | Tier II |
12 | NC_000068.7 | 2 | 62398148 | - | Dpp4 | NNNGCAGAGATGGCAAACAG | NGG | 2 | 0.2435 | Tier II |
13 | NC_000080.6 | 14 | 10427182 | - | Fhit | NNNGCAGAGATGAAAAGCAG | NGG | 2 | 0.2423 | Tier II |
14 | NC_000083.6 | 17 | 51004448 | - | Tbc1d5 | NNNGCAGAGTTGTCAAGCAG | NAG | 2 | 0.2288 | Tier II |
15 | NC_000076.6 | 10 | 50782034 | - | Ascc3 | NNNGCAGAGATGCCCAGCAG | NGG | 2 | 0.2153 | Tier II |
16 | NC_000080.6 | 14 | 93623105 | - | Pcdh9 | NNNGCAGAGATCTAAAGCAG | NGG | 2 | 0.1853 | Tier II |
17 | NC_000075.6 | 9 | 29381293 | - | Ntm | NNNGCAGAGTTGTCAGGCAG | NGG | 2 | 0.1697 | Tier II |
18 | NC_000084.6 | 18 | 75993585 | - | Zbtb7c | NNNGCAGAGATGCCAGGCAG | NGG | 2 | 0.1518 | Tier II |
19 | NC_000073.6 | 7 | 6383778 | + | Zfp28 | NNNGCAGAGAAGTCAGGCAG | NGG | 2 | 0.1442 | Tier II |
20 | NC_000075.6 | 9 | 108839140 | - | Celsr3 | NNNGCAGAGATCTCAAGCAG | NAG | 2 | 0.1373 | Tier II |
21 | NC_000078.6 | 12 | 18962262 | - | Gm40847 | NNNGCAGTGATGTCAAGCAG | NAG | 2 | 0.1111 | Tier II |
22 | NC_000070.6 | 4 | 141202688 | - | Fbxo42 | NNNGCAGAGATCTCAGGCAG | NGG | 2 | 0.1018 | Tier II |
23 | NC_000079.6 | 13 | 95739381 | - | Iqgap2 | NNNTCAGAGATGTCAACCAG | NGG | 2 | 0.0856 | Tier II |
24 | NC_000067.6 | 1 | 105298788 | + | Rnf152 | NNNGCAGTGATGTCAGGCAG | NGG | 2 | 0.0824 | Tier II |
25 | NC_000069.6 | 3 | 22154237 | - | Tbl1xr1 | NNNGCAGAGATGTCAATCAG | NAG | 2 | 0.0648 | Tier II |
26 | NC_000078.6 | 12 | 81976537 | - | Pcnx | NNNGTAGAGATGTCAAGCAG | NGA | 2 | 0.0442 | Tier II |
27 | NC_000079.6 | 13 | 37797481 | - | Rreb1 | NNNGCAGAGATGTCAAGCTG | NTG | 2 | 0.021 | Tier II |
28 | NC_000079.6 | 13 | 97129231 | + | Fam169a | NNNGCAGAGATTTCAAGCAG | NTG | 2 | 0.015 | Tier II |
29 | NC_000074.6 | 8 | 111490246 | + | Wdr59 | NNNGCAGAGATGTCCAGCAG | NGC | 2 | 0.0061 | Tier II |
30 | NC_000068.7 | 2 | 20853639 | - | Arhgap21 | NNNGCAGAGATGTGAAGCTG | NGG | 2 | 0.0 | Tier II |
31 | NC_000069.6 | 3 | 139008224 | - | Rap1gds1 | NNNGCAGAGATGTGAGGCAG | NGG | 2 | 0.0 | Tier II |
32 | NC_000081.6 | 15 | 13154228 | + | Cdh6 | NNNGCAGAGATTTGAAGCAG | NGG | 2 | 0.0 | Tier II |
33 | NC_000070.6 | 4 | 136335164 | + | Hnrnpr | NNNTCAGAGATGTGAAGCAG | NGG | 2 | 0.0 | Tier II |
34 | NC_000084.6 | 18 | 68610087 | - | Gm30821 | NNNGCAGGGATGTCAAACAG | NGG | 2 | 0.6844 | Tier III |
35 | NC_000075.6 | 9 | 90476596 | - | Gm39398 | NNNGCAGACATGACAAGCAG | NGG | 2 | 0.3728 | Tier III |
36 | NC_000069.6 | 3 | 64723792 | + | Vmn2r-ps11 | NNNGCAGAGCTGCCAAGCAG | NGG | 2 | 0.2632 | Tier III |
37 | NC_000067.6 | 1 | 120717335 | + | 2610027F03Rik | NNNGCAGGGATGTCAAGCAG | NAG | 2 | 0.1901 | Tier III |
38 | NC_000079.6 | 13 | 53477862 | + | Gm33763 | NNNGTAGAGATGTCAACCAG | NGG | 2 | 0.1497 | Tier III |
39 | NC_000079.6 | 13 | 37797481 | - | Gm46411 | NNNGCAGAGATGTCAAGCTG | NTG | 2 | 0.021 | Tier III |
40 | NC_000072.6 | 6 | 69979037 | + | Igk | NNNGCAGACATGTGAAGCAG | NGG | 2 | 0.0 | Tier III |