Construct: sgRNA BRDN0001146411
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTGAGTCTCTACCTGAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RYK (6259)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76451
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
134250450 |
- |
RYK |
NNNGAGTCTCTACCTGAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
125366607 |
- |
NSMCE2 |
NNNGAGTCCCTACCTGAGCC |
NGG |
2 |
0.3956 |
Tier II |
| 3 |
NC_000017.11 |
17 |
4222284 |
+ |
ANKFY1 |
NNNGAGCCTCTACCTGAGTG |
NGG |
2 |
0.3173 |
Tier II |
| 4 |
NC_000014.9 |
14 |
95359948 |
+ |
LOC107984710 |
NNNGAGTCCCTACCTGAGCC |
NGG |
2 |
0.3956 |
Tier III |
| 5 |
NC_000002.12 |
2 |
125755371 |
- |
LINC01889 |
NNNCAGTCTCTGCCTGAGCG |
NGG |
2 |
0.3824 |
Tier III |
| 6 |
NC_000002.12 |
2 |
125755371 |
- |
LOC124900611 |
NNNCAGTCTCTGCCTGAGCG |
NGG |
2 |
0.3824 |
Tier III |
| 7 |
NC_000017.11 |
17 |
4222284 |
+ |
RYKP1 |
NNNGAGCCTCTACCTGAGTG |
NGG |
2 |
0.3173 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
102835213 |
+ |
Ryk |
NNNGAGCCTCTACCTGAGCG |
NGG |
1 |
0.6875 |
Tier I |
| 2 |
NC_000074.6 |
8 |
10382781 |
- |
Myo16 |
NNNGAGTTTCTACCTGAGCA |
NGG |
2 |
0.8203 |
Tier II |
| 3 |
NC_000068.7 |
2 |
32819813 |
+ |
Stxbp1 |
NNNGAGTCTCAGCCTGAGCG |
NGG |
2 |
0.5417 |
Tier II |
| 4 |
NC_000070.6 |
4 |
149758922 |
+ |
Slc25a33 |
NNNCAGTGTCTACCTGAGCG |
NGG |
2 |
0.3403 |
Tier II |
| 5 |
NC_000077.6 |
11 |
117853352 |
- |
Birc5 |
NNNGAGCCTCTACCTGAGCC |
NGG |
2 |
0.2946 |
Tier II |
| 6 |
NC_000080.6 |
14 |
12122387 |
- |
Ptprg |
NNNGGGTCTCTCCCTGAGCG |
NGG |
2 |
0.1895 |
Tier II |
| 7 |
NC_000075.6 |
9 |
86819494 |
- |
Snap91 |
NNNGAGTCTCTCCCTGAGCC |
NGG |
2 |
0.1128 |
Tier II |
| 8 |
NC_000082.6 |
16 |
72320522 |
- |
Robo1 |
NNNGAGTCTCTCCCTGAGCC |
NGG |
2 |
0.1128 |
Tier II |
| 9 |
NC_000075.6 |
9 |
113788827 |
+ |
Clasp2 |
NNNGAGTCTCTGGCTGAGCG |
NGG |
2 |
0.0985 |
Tier II |
| 10 |
NC_000075.6 |
9 |
114617155 |
- |
Cnot10 |
NNNGAGTCTCTGGCTGAGCG |
NGG |
2 |
0.0985 |
Tier II |
| 11 |
NC_000074.6 |
8 |
23993570 |
+ |
Zmat4 |
NNNGAGTCTCTTCCTGAGGG |
NGG |
2 |
0.0417 |
Tier II |
| 12 |
NC_000067.6 |
1 |
90154507 |
- |
4930434B07Rik |
NNNGAGTCTCTTCCTGAGCC |
NGG |
2 |
0.1429 |
Tier III |
| 13 |
NC_000068.7 |
2 |
173356609 |
- |
Gm36911 |
NNNGAGTCTCTCACTGAGCG |
NGG |
2 |
0.1012 |
Tier III |
Other clones with same target sequence:
(none)
