Construct: sgRNA BRDN0001146412
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACACGGTGTATGCTGTGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IRAK1 (3654)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76421
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
154018614 |
- |
IRAK1 |
NNNCGGTGTATGCTGTGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
129246062 |
- |
TMEM132D |
NNNCAGTGGATGCTGTGAAG |
NGG |
2 |
0.5365 |
Tier II |
| 3 |
NC_000003.12 |
3 |
67598703 |
+ |
SUCLG2 |
NNNCAGTGTATGCTGTGATG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000011.10 |
11 |
126605442 |
+ |
KIRREL3 |
NNNCGGTGACTGCTGTGAAG |
NGG |
2 |
0.3095 |
Tier II |
| 5 |
NC_000001.11 |
1 |
182387080 |
- |
GLUL |
NNNCTGTGAATGCTGTGAAG |
NGG |
2 |
0.2786 |
Tier II |
| 6 |
NC_000010.11 |
10 |
72013253 |
- |
CHST3 |
NNNCAGTGTATGCCGTGAAG |
NGG |
2 |
0.2476 |
Tier II |
| 7 |
NC_000001.11 |
1 |
26353764 |
+ |
CRYBG2 |
NNNTGGTGTATGCTGTGAAG |
NAG |
2 |
0.2074 |
Tier II |
| 8 |
NC_000004.12 |
4 |
80411932 |
+ |
CFAP299 |
NNNCGGTGTATTCTGTGCAG |
NGG |
2 |
0.0733 |
Tier II |
| 9 |
NC_000016.10 |
16 |
33691777 |
- |
BMS1P8 |
NNNCGGTGTATGCTGTGATG |
NGG |
1 |
0.5385 |
Tier III |
| 10 |
NC_000011.10 |
11 |
126605442 |
+ |
KIRREL3-AS1 |
NNNCGGTGACTGCTGTGAAG |
NGG |
2 |
0.3095 |
Tier III |
| 11 |
NC_000002.12 |
2 |
94192885 |
- |
BMS1P23 |
NNNGGGTGTATGCTGTGATG |
NGG |
2 |
0.2692 |
Tier III |
| 12 |
NC_000009.12 |
9 |
40568690 |
- |
LOC102724580 |
NNNGGGTGTATGCTGTGATG |
NGG |
2 |
0.2692 |
Tier III |
| 13 |
NC_000009.12 |
9 |
63369601 |
- |
LOC100996643 |
NNNGGGTGTATGCTGTGATG |
NGG |
2 |
0.2692 |
Tier III |
| 14 |
NC_000022.11 |
22 |
22313565 |
+ |
IGL |
NNNGGGTGTATGCTGTGATG |
NGG |
2 |
0.2692 |
Tier III |
| 15 |
NC_000022.11 |
22 |
22313565 |
+ |
BMS1P20 |
NNNGGGTGTATGCTGTGATG |
NGG |
2 |
0.2692 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
33935377 |
+ |
Rgl2 |
NNNCCGTGTATCCTGTGAAG |
NGG |
2 |
0.416 |
Tier I |
| 2 |
NC_000069.6 |
3 |
107507713 |
- |
Slc6a17 |
NNNCGGAGTATGCTGAGAAG |
NGG |
2 |
0.7955 |
Tier II |
| 3 |
NC_000071.6 |
5 |
9456727 |
- |
Elapor2 |
NNNAGGTGTATACTGTGAAG |
NGG |
2 |
0.786 |
Tier II |
| 4 |
NC_000077.6 |
11 |
37137916 |
+ |
Tenm2 |
NNNTGGTGAATGCTGTGAAG |
NGG |
2 |
0.7429 |
Tier II |
| 5 |
NC_000075.6 |
9 |
56881297 |
- |
Cspg4 |
NNNTGGTGTAGGCTGTGAAG |
NGG |
2 |
0.32 |
Tier II |
| 6 |
NC_000074.6 |
8 |
85945535 |
+ |
Phkb |
NNNCAGTGTATGCTGTGCAG |
NGG |
2 |
0.1651 |
Tier II |
| 7 |
NC_000076.6 |
10 |
40664559 |
+ |
Ddo |
NNNCGGTGTGTGCTGTGAAG |
NAG |
2 |
0.144 |
Tier II |
| 8 |
NC_000075.6 |
9 |
70140406 |
- |
Fam81a |
NNNCTGTGTATGCTGTTAAG |
NGG |
2 |
0.075 |
Tier II |
Other clones with same target sequence:
(none)
