Construct: sgRNA BRDN0001146414
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAACCATATTAGGCTTGGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA5 (2044)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76676
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
65377011 |
+ |
EPHA5 |
NNNCCATATTAGGCTTGGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
63317027 |
+ |
TANC2 |
NNNTCATATTAAGCTTGGAT |
NGG |
2 |
0.7467 |
Tier II |
| 3 |
NC_000017.11 |
17 |
44177526 |
+ |
ASB16 |
NNNCCAGATTAGGCTAGGAT |
NGG |
2 |
0.5348 |
Tier II |
| 4 |
NC_000009.12 |
9 |
70970986 |
- |
TRPM3 |
NNNCCAGTTTAGGCTTGGAT |
NGG |
2 |
0.2521 |
Tier II |
| 5 |
NC_000019.10 |
19 |
9037964 |
- |
MUC16 |
NNNCCATATTAGGCTTGGAG |
NCG |
2 |
0.0189 |
Tier II |
| 6 |
NC_000017.11 |
17 |
44177526 |
+ |
ASB16-AS1 |
NNNCCAGATTAGGCTAGGAT |
NGG |
2 |
0.5348 |
Tier III |
| 7 |
NC_000011.10 |
11 |
95477981 |
- |
LOC105369439 |
NNNCAATATTAAGCTTGGAT |
NGG |
2 |
0.5333 |
Tier III |
| 8 |
NC_000023.11 |
X |
22367102 |
- |
PTCHD1-AS |
NNNCCATATTAGGCTTGGAT |
NCA |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
84117574 |
+ |
Epha5 |
NNNCCATATTAGGCTTGGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000074.6 |
8 |
83319863 |
+ |
Elmod2 |
NNNCCATATTAGGCTTGAAT |
NGG |
1 |
0.6923 |
Tier II |
| 3 |
NC_000076.6 |
10 |
86721004 |
+ |
Ttc41 |
NNNACATGTTAGGCTTGGAT |
NGG |
2 |
0.6175 |
Tier II |
| 4 |
NC_000067.6 |
1 |
40548309 |
- |
Il18rap |
NNNCCATTTGAGGCTTGGAT |
NGG |
2 |
0.2143 |
Tier II |
| 5 |
NC_000069.6 |
3 |
28196949 |
+ |
Gm42196 |
NNNTCATATTAGACTTGGAT |
NGG |
2 |
0.7385 |
Tier III |
| 6 |
NC_000078.6 |
12 |
114225462 |
+ |
Igh |
NNNCCATATTAGCCTTGTAT |
NGG |
2 |
0.2807 |
Tier III |
| 7 |
NC_000081.6 |
15 |
102997610 |
+ |
Gm29905 |
NNNCCATCTTAGGCTTGGTT |
NGG |
2 |
0.2308 |
Tier III |
| 8 |
NC_000068.7 |
2 |
105077293 |
+ |
Wt1os |
NNNCCATATGTGGCTTGGAT |
NGG |
2 |
0.1538 |
Tier III |
Other clones with same target sequence:
(none)
