Construct: sgRNA BRDN0001146419

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGTTGACTACATGCATGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK11 (79858)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75562

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 131109917 + NEK11 NNNTTGACTACATGCATGAG NGG 0 1.0 Tier I
2 NC_000017.11 17 35557763 - SLFN14 NNNTTGACTACATGCAGCAG NGG 2 0.056 Tier I
3 NC_000009.12 9 130613394 + FUBP3 NNNTTGACTACATGCATCAG NGG 1 0.4762 Tier II
4 NC_000014.9 14 81403540 + STON2 NNNTTGAATACATGCAAGAG NGG 2 0.3467 Tier II
5 NC_000020.11 20 8434946 - PLCB1 NNNTTGTCTACGTGCATGAG NGG 2 0.316 Tier II
6 NC_000002.12 2 73995292 + TET3 NNNATGACTACATGCAAGAG NGG 2 0.254 Tier II
7 NC_000003.12 3 48522690 - PFKFB4 NNNTTGCCTACCTGCATGAG NGG 2 0.1151 Tier II
8 NC_000005.10 5 96392470 + CAST NNNTTGACTACAGGAATGAG NGG 2 0.058 Tier II
9 NC_000005.10 5 96392470 + PCSK1 NNNTTGACTACAGGAATGAG NGG 2 0.058 Tier II
10 NC_000016.10 16 83039709 + CDH13 NNNTTGAATACATGGATGAG NGG 2 0.0325 Tier II
11 NC_000008.11 8 119993034 + DEPTOR NNNTGGACTACATGCATGAG NTG 2 0.0249 Tier II
12 NC_000016.10 16 79797358 - LINC01229 NNNTTGAATTCATGCATGAG NGG 2 0.5735 Tier III
13 NC_000020.11 20 8434946 - LOC124900459 NNNTTGTCTACGTGCATGAG NGG 2 0.316 Tier III
14 NC_000005.10 5 96392470 + LOC101929710 NNNTTGACTACAGGAATGAG NGG 2 0.058 Tier III
15 NC_000014.9 14 49088786 - LOC105378178 NNNTTGAATACATGGATGAG NGG 2 0.0325 Tier III
16 NC_000003.12 3 54120511 + LOC124909381 NNNTTGACTACATGAATGAG NGA 2 0.0154 Tier III
17 NC_000005.10 5 152651005 - LINC01470 NNNTTGACTACATGCTTTAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 105323123 - Nek11 NNNTTGACTACATGCATGAG NGG 0 1.0 Tier I
2 NC_000074.6 8 107455073 + Wwp2 NNNTTTACTACATGCATGAA NGG 2 0.625 Tier II
3 NC_000082.6 16 37781216 - Fstl1 NNNTTAACTACATGCATGTG NGG 2 0.5385 Tier II
4 NC_000085.6 19 46964727 - Nt5c2 NNNTTGCCTACATGCATGAG NGG 1 0.4375 Tier II
5 NC_000068.7 2 5599150 + Camk1d NNNATGAGTACATGCATGAG NGG 2 0.3061 Tier II
6 NC_000077.6 11 72790999 + Cyb5d2 NNNATGAGTACATGCATGAG NGG 2 0.3061 Tier II
7 NC_000078.6 12 85968855 + Ttll5 NNNATGACTACATGCATGTG NGG 2 0.2564 Tier II
8 NC_000086.7 X 12130986 - Bcor NNNTTGAATCCATGCATGAG NGG 2 0.2167 Tier II
9 NC_000076.6 10 59566974 + Mcu NNNTTGACTGCATGAATGAG NGG 2 0.1235 Tier II
10 NC_000073.6 7 115922499 - Sox6 NNNTTGACTACATGAATCAG NGG 2 0.1058 Tier II
11 NC_000086.7 X 102798642 + Dmrtc1c1 NNNTTGACTACATGCGTGAG NAG 2 0.0499 Tier II
12 NC_000068.7 2 144406309 - Kat14 NNNTTTACTACATGCATGAG NGA 2 0.0463 Tier II
13 NC_000071.6 5 131716096 - Auts2 NNNTTGACTACAAGTATGAG NGG 2 0.0462 Tier II
14 NC_000067.6 1 86588416 - Cops7b NNNTTGACTGCATGTATGAG NGG 2 0.037 Tier II
15 NC_000067.6 1 157274596 + Rasal2 NNNTTAACTACATGCATGAG NGT 2 0.0161 Tier II
16 NC_000079.6 13 116949818 + Parp8 NNNTTGGCTACATGCATGAG NGC 2 0.0157 Tier II
17 NC_000072.6 6 142758572 - Cmas NNNGTGACTACATGCATGAG NGC 2 0.0139 Tier II
18 NC_000073.6 7 56277681 - Oca2 NNNTTGTCTACATGCATGAG NGC 2 0.0097 Tier II
19 NC_000078.6 12 114694301 - Igh NNNTTGAGTACATGCATGGG NGG 2 0.2411 Tier III
20 NC_000067.6 1 158974080 - 1600012P17Rik NNNTTGACTCCAGGCATGAG NGG 2 0.087 Tier III
21 NC_000070.6 4 50864987 + Gm42287 NNNTTGCCTACATGTATGAG NGG 2 0.0292 Tier III
22 NC_000072.6 6 142758572 - LOC115490443 NNNGTGACTACATGCATGAG NGC 2 0.0139 Tier III
23 NC_000069.6 3 78407965 - Gm36288 NNNTTGACTACATGCATGAT NGT 2 0.0113 Tier III
24 NC_000078.6 12 115959777 - Igh NNNTTGACTACATGCTTGGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)