Construct: sgRNA BRDN0001146427

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATGCAACTTCACAATATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA5 (9252)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77373

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 90899363 + RPS6KA5 NNNGCAACTTCACAATATTG NGG 0 1.0 Tier I
2 NC_000002.12 2 185745483 - FSIP2 NNNGCAACATCACAATGTTG NGG 2 0.1639 Tier I
3 NC_000013.11 13 24499337 + PARP4 NNNGCAACTTCAAAATCTTG NGG 2 0.0679 Tier I
4 NC_000004.12 4 151237773 + SH3D19 NNNGCAAGTTCATAATATTG NGG 2 0.45 Tier II
5 NC_000002.12 2 77437082 - LRRTM4 NNNGCAAATTCACTATATTG NGG 2 0.3467 Tier II
6 NC_000003.12 3 41284148 + ULK4 NNNTCAATTTCACAATATTG NGG 2 0.3182 Tier II
7 NC_000001.11 1 8701833 - RERE NNNGCAGCTTCACAATAGTG NGG 2 0.2353 Tier II
8 NC_000003.12 3 51145927 - DOCK3 NNNTGAACTTCACAATATTG NGG 2 0.2182 Tier II
9 NC_000010.11 10 12949092 + CCDC3 NNNGCAACATCACAATTTTG NGG 2 0.1238 Tier II
10 NC_000004.12 4 150138538 + DCLK2 NNNCCAACTTCACATTATTG NGG 2 0.1059 Tier II
11 NC_000004.12 4 61474884 - ADGRL3 NNNGCAACTTCAAAATCTTG NGG 2 0.0679 Tier II
12 NC_000008.11 8 12722868 + LONRF1 NNNGCAACTTCACAATGTAG NGG 2 0.0504 Tier II
13 NC_000012.12 12 77811879 - NAV3 NNNGCAAATTCACAATATTG NGA 2 0.0451 Tier II
14 NC_000008.11 8 143354953 - TOP1MT NNNGCAACTTCACAAAATTG NGT 2 0.0147 Tier II
15 NC_000009.12 9 99963071 - STX17 NNNGTAACTTCACAATATTG NGC 2 0.0141 Tier II
16 NC_000016.10 16 60486244 + LOC101927605 NNNAAAACTTCACAATATTG NGG 2 0.5143 Tier III
17 NC_000009.12 9 1334607 - LOC102723803 NNNTCATCTTCACAATATTG NGG 2 0.1591 Tier III
18 NC_000022.11 22 47818158 - EPIC1 NNNGCAACTTCCCAATACTG NGG 2 0.1128 Tier III
19 NC_000006.12 6 133580207 + TARID NNNGAAACTTCACAATTTTG NGG 2 0.0762 Tier III
20 NC_000013.11 13 19350188 - PARP4P2 NNNGCAACTTCAAAATCTTG NGG 2 0.0679 Tier III
21 NC_000008.11 8 88567715 + LOC105375629 NNNGCAACTTCACAATATTC NGA 2 0.0298 Tier III
22 NC_000008.11 8 88567715 + LOC105375630 NNNGCAACTTCACAATATTC NGA 2 0.0298 Tier III
23 NC_000001.11 1 200940039 + MROH3P NNNACAACTTCACAATATTG NGT 2 0.0145 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 140265744 + Sel1l2 NNNGCAACTTCACAATTTTG NAG 2 0.0346 Tier I
2 NC_000072.6 6 61280247 - Ccser1 NNNACAACTTCACATTATTG NGG 2 0.18 Tier II
3 NC_000076.6 10 85473260 - Btbd11 NNNGCAAATTCACAATATGG NGG 2 0.1625 Tier II
4 NC_000081.6 15 48544229 - Csmd3 NNNGCAACTTCACAATATTT NGA 2 0.0486 Tier II
5 NC_000076.6 10 94560524 + Tmcc3 NNNGCAACTTCACAAGTTTG NGG 2 0.0462 Tier II
6 NC_000086.7 X 43333418 - Tenm1 NNNGCAAATTCACAATATTG NGC 2 0.0144 Tier II
7 NC_000069.6 3 145362929 - Col24a1 NNNGCAACTTCACAAGATTG NTG 2 0.0135 Tier II
8 NC_000071.6 5 103078631 + Mapk10 NNNCCAACTTCACAATATTG NGC 2 0.0118 Tier II
9 NC_000067.6 1 19096480 - Gm15825 NNNGCAACTTCACAAAAGTG NGG 2 0.303 Tier III
10 NC_000071.6 5 91864785 - Gm36357 NNNGGAACTGCACAATATTG NGG 2 0.3 Tier III
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Other clones with same target sequence:

(none)