Construct: sgRNA BRDN0001146434

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGATGACATGAAACACCTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACTR2 (10097)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76955

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 65246627 + ACTR2 NNNTGACATGAAACACCTGT NGG 0 1.0 Tier I
2 NC_000004.12 4 141231438 - ZNF330 NNNTTGCATGAAACACCTGT NGG 2 0.2143 Tier I
3 NC_000002.12 2 60921810 - REL NNNTGACTTGAAACCCCTGT NGG 2 0.1169 Tier I
4 NC_000013.11 13 100512172 - PCCA NNNTGACAAGAAACACCAGT NGG 2 0.619 Tier II
5 NC_000008.11 8 120573336 + SNTB1 NNNTGACATTAAACACCTTT NGG 2 0.5417 Tier II
6 NC_000012.12 12 2672299 + CACNA1C NNNTGACATGAAACAATTGT NGG 2 0.4667 Tier II
7 NC_000008.11 8 69557854 - SULF1 NNNTGACCTGGAACACCTGT NGG 2 0.2786 Tier II
8 NC_000002.12 2 95890360 - ANKRD36C NNNTTACATGAAACACATGT NGG 2 0.14 Tier II
9 NC_000005.10 5 96297526 + CAST NNNTGACATGAAACAGCTCT NGG 2 0.069 Tier II
10 NC_000004.12 4 8114943 + ABLIM2 NNNTGACATGAGACACCTGC NGG 2 0.0657 Tier II
11 NC_000010.11 10 21236140 - NEBL NNNTGATATGAAACACCTGT NGA 2 0.0564 Tier II
12 NC_000019.10 19 20258496 + ACTR2P2 NNNTGACACGAAACACCTGT NGG 1 0.9231 Tier III
13 NC_000019.10 19 20258496 + LOC105372310 NNNTGACACGAAACACCTGT NGG 1 0.9231 Tier III
14 NC_000015.10 15 21574092 - LINC02203 NNNTGAAATTAAACACCTGT NGG 2 0.6094 Tier III
15 NC_000015.10 15 22009414 - OR4M2-OT1 NNNTGAAATTAAACACCTGT NGG 2 0.6094 Tier III
16 NC_000022.11 22 24687185 + ARL5AP4 NNNTGACGTGAAAAACCTGT NGG 2 0.2567 Tier III
17 NC_000022.11 22 24687185 + ACTR2P1 NNNTGACGTGAAAAACCTGT NGG 2 0.2567 Tier III
18 NC_000024.10 Y 21152704 - LOC105377225 NNNTGAAATGAAACAGCTGT NGG 2 0.1154 Tier III
19 NC_000003.12 3 142976281 - PAQR9-AS1 NNNTTACATGAAACATCTGT NGG 2 0.0923 Tier III
20 NC_000005.10 5 96297526 + LOC101929710 NNNTGACATGAAACAGCTCT NGG 2 0.069 Tier III
21 NC_000006.12 6 114403080 + LOC107986638 NNNTGACATGAAAAAGCTGT NGG 2 0.0538 Tier III
22 NC_000008.11 8 75134809 - LOC105375905 NNNTGACATGAAACTCCTGT NGC 2 0.0044 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 20094343 - Actr2 NNNTGACATGAAGCACCTGT NGG 1 0.6522 Tier I
2 NC_000083.6 17 43602937 + Pla2g7 NNNTGACATGAAACAGCTGA NGG 2 0.0865 Tier I
3 NC_000072.6 6 86888676 - Aak1 NNNTGACAAGAAGCACCTGT NGG 2 0.6056 Tier II
4 NC_000067.6 1 124158917 + Dpp10 NNNTGAGATAAAACACCTGT NGG 2 0.4392 Tier II
5 NC_000081.6 15 93516538 + Prickle1 NNNTGACATGAGACCCCTGT NGG 2 0.197 Tier II
6 NC_000067.6 1 93729782 + Thap4 NNNTGACATGAAATACCTGT NAG 2 0.1901 Tier II
7 NC_000068.7 2 155817787 + Mmp24 NNNTCACATGAAACTCCTGT NGG 2 0.1571 Tier II
8 NC_000068.7 2 155817787 + BC029722 NNNTCACATGAAACTCCTGT NGG 2 0.1571 Tier II
9 NC_000077.6 11 20711610 - Aftph NNNTGACATGTAACACTTGT NGG 2 0.1436 Tier II
10 NC_000071.6 5 31305160 - Gckr NNNTGACATGAAACACCTTG NGG 2 0.1176 Tier II
11 NC_000075.6 9 42938607 - Grik4 NNNTGACATGCAACCCCTGT NGG 2 0.1091 Tier II
12 NC_000085.6 19 59298091 + Pdzd8 NNNTGACCTGAAACTCCTGT NGG 2 0.0857 Tier II
13 NC_000080.6 14 59708579 - Atp8a2 NNNTGACATGAAACACTTGG NGG 2 0.0824 Tier II
14 NC_000073.6 7 27514929 + Prx NNNTTACATGAAACCCCTGT NGG 2 0.0818 Tier II
15 NC_000077.6 11 115654205 + Grb2 NNNTGACATGAAACACTTGT NGA 2 0.0324 Tier II
16 NC_000068.7 2 49487134 + Epc2 NNNTGACATGAAACACCTAT NGC 2 0.0159 Tier II
17 NC_000082.6 16 37852059 + Gm36028 NNNTGACATGCAACACCTGT NTG 2 0.0156 Tier II
18 NC_000071.6 5 131275615 + Galnt17 NNNTGACATGAAACACCTGA NGC 2 0.0125 Tier II
19 NC_000082.6 16 97815161 - Prdm15 NNNTGACATGAACCACCTGT NTG 2 0.0082 Tier II
20 NC_000086.7 X 79642923 + Mageb16 NNNTGACATGACACACCTGT NGC 2 0.0058 Tier II
21 NC_000085.6 19 11990107 - Osbp NNNTGACATGAACCACCTGT NGT 2 0.0034 Tier II
22 NC_000075.6 9 110272345 + Gm39429 NNNTAACATGAAGCACCTGT NGG 2 0.5652 Tier III
23 NC_000080.6 14 102179478 - Gm41232 NNNTGACAGCAAACACCTGT NGG 2 0.2476 Tier III
24 NC_000073.6 7 7097767 - Gm6828 NNNTGACATGAAGCCCCTGT NGG 2 0.1779 Tier III
25 NC_000077.6 11 67140017 - Gm31285 NNNTGATATGAAACACCTGG NGG 2 0.1434 Tier III
26 NC_000073.6 7 138545678 - Gm30751 NNNTGACATGAAACATCTGT NTG 2 0.012 Tier III
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Other clones with same target sequence:

(none)