Construct: sgRNA BRDN0001146434
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGATGACATGAAACACCTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACTR2 (10097)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76955
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000077.6 |
11 |
20094343 |
- |
Actr2 |
NNNTGACATGAAGCACCTGT |
NGG |
1 |
0.6522 |
Tier I |
2 |
NC_000083.6 |
17 |
43602937 |
+ |
Pla2g7 |
NNNTGACATGAAACAGCTGA |
NGG |
2 |
0.0865 |
Tier I |
3 |
NC_000072.6 |
6 |
86888676 |
- |
Aak1 |
NNNTGACAAGAAGCACCTGT |
NGG |
2 |
0.6056 |
Tier II |
4 |
NC_000067.6 |
1 |
124158917 |
+ |
Dpp10 |
NNNTGAGATAAAACACCTGT |
NGG |
2 |
0.4392 |
Tier II |
5 |
NC_000081.6 |
15 |
93516538 |
+ |
Prickle1 |
NNNTGACATGAGACCCCTGT |
NGG |
2 |
0.197 |
Tier II |
6 |
NC_000067.6 |
1 |
93729782 |
+ |
Thap4 |
NNNTGACATGAAATACCTGT |
NAG |
2 |
0.1901 |
Tier II |
7 |
NC_000068.7 |
2 |
155817787 |
+ |
Mmp24 |
NNNTCACATGAAACTCCTGT |
NGG |
2 |
0.1571 |
Tier II |
8 |
NC_000068.7 |
2 |
155817787 |
+ |
BC029722 |
NNNTCACATGAAACTCCTGT |
NGG |
2 |
0.1571 |
Tier II |
9 |
NC_000077.6 |
11 |
20711610 |
- |
Aftph |
NNNTGACATGTAACACTTGT |
NGG |
2 |
0.1436 |
Tier II |
10 |
NC_000071.6 |
5 |
31305160 |
- |
Gckr |
NNNTGACATGAAACACCTTG |
NGG |
2 |
0.1176 |
Tier II |
11 |
NC_000075.6 |
9 |
42938607 |
- |
Grik4 |
NNNTGACATGCAACCCCTGT |
NGG |
2 |
0.1091 |
Tier II |
12 |
NC_000085.6 |
19 |
59298091 |
+ |
Pdzd8 |
NNNTGACCTGAAACTCCTGT |
NGG |
2 |
0.0857 |
Tier II |
13 |
NC_000080.6 |
14 |
59708579 |
- |
Atp8a2 |
NNNTGACATGAAACACTTGG |
NGG |
2 |
0.0824 |
Tier II |
14 |
NC_000073.6 |
7 |
27514929 |
+ |
Prx |
NNNTTACATGAAACCCCTGT |
NGG |
2 |
0.0818 |
Tier II |
15 |
NC_000077.6 |
11 |
115654205 |
+ |
Grb2 |
NNNTGACATGAAACACTTGT |
NGA |
2 |
0.0324 |
Tier II |
16 |
NC_000068.7 |
2 |
49487134 |
+ |
Epc2 |
NNNTGACATGAAACACCTAT |
NGC |
2 |
0.0159 |
Tier II |
17 |
NC_000082.6 |
16 |
37852059 |
+ |
Gm36028 |
NNNTGACATGCAACACCTGT |
NTG |
2 |
0.0156 |
Tier II |
18 |
NC_000071.6 |
5 |
131275615 |
+ |
Galnt17 |
NNNTGACATGAAACACCTGA |
NGC |
2 |
0.0125 |
Tier II |
19 |
NC_000082.6 |
16 |
97815161 |
- |
Prdm15 |
NNNTGACATGAACCACCTGT |
NTG |
2 |
0.0082 |
Tier II |
20 |
NC_000086.7 |
X |
79642923 |
+ |
Mageb16 |
NNNTGACATGACACACCTGT |
NGC |
2 |
0.0058 |
Tier II |
21 |
NC_000085.6 |
19 |
11990107 |
- |
Osbp |
NNNTGACATGAACCACCTGT |
NGT |
2 |
0.0034 |
Tier II |
22 |
NC_000075.6 |
9 |
110272345 |
+ |
Gm39429 |
NNNTAACATGAAGCACCTGT |
NGG |
2 |
0.5652 |
Tier III |
23 |
NC_000080.6 |
14 |
102179478 |
- |
Gm41232 |
NNNTGACAGCAAACACCTGT |
NGG |
2 |
0.2476 |
Tier III |
24 |
NC_000073.6 |
7 |
7097767 |
- |
Gm6828 |
NNNTGACATGAAGCCCCTGT |
NGG |
2 |
0.1779 |
Tier III |
25 |
NC_000077.6 |
11 |
67140017 |
- |
Gm31285 |
NNNTGATATGAAACACCTGG |
NGG |
2 |
0.1434 |
Tier III |
26 |
NC_000073.6 |
7 |
138545678 |
- |
Gm30751 |
NNNTGACATGAAACATCTGT |
NTG |
2 |
0.012 |
Tier III |
Other clones with same target sequence:
(none)