Construct: sgRNA BRDN0001146436
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCTTACGTGGGGGGCAAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80221
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
150746678 |
+ |
SIAH2 |
NNNTCACGTAGGGGGCAAAA |
NGG |
2 |
0.9333 |
Tier II |
| 2 |
NC_000002.12 |
2 |
29554247 |
+ |
ALK |
NNNTTAAGTGGGGGGAAAAA |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000013.11 |
13 |
26330567 |
- |
CDK8 |
NNNTTAAGTGGGAGGCAAAA |
NGG |
2 |
0.6923 |
Tier II |
| 4 |
NC_000019.10 |
19 |
31191497 |
- |
TSHZ3 |
NNNTTACTTTGGGGGCAAAA |
NGG |
2 |
0.5078 |
Tier II |
| 5 |
NC_000006.12 |
6 |
143726437 |
- |
PHACTR2 |
NNNTTACTGGGGGGGCAAAA |
NGG |
2 |
0.3869 |
Tier II |
| 6 |
NC_000007.14 |
7 |
1839173 |
+ |
MAD1L1 |
NNNTTAGGTTGGGGGCAAAA |
NGG |
2 |
0.3824 |
Tier II |
| 7 |
NC_000002.12 |
2 |
23871150 |
- |
ATAD2B |
NNNTTATGTGGTGGGCAAAA |
NGG |
2 |
0.3125 |
Tier II |
| 8 |
NC_000010.11 |
10 |
12908221 |
+ |
CCDC3 |
NNNTTACGTGGGGACCAAAA |
NGG |
2 |
0.2045 |
Tier II |
| 9 |
NC_000003.12 |
3 |
1296251 |
+ |
CNTN6 |
NNNTTACGTGGCTGGCAAAA |
NGG |
2 |
0.1588 |
Tier II |
| 10 |
NC_000019.10 |
19 |
31191497 |
- |
LINC01791 |
NNNTTACTTTGGGGGCAAAA |
NGG |
2 |
0.5078 |
Tier III |
| 11 |
NC_000007.14 |
7 |
1839173 |
+ |
LOC100127955 |
NNNTTAGGTTGGGGGCAAAA |
NGG |
2 |
0.3824 |
Tier III |
| 12 |
NC_000010.11 |
10 |
12908221 |
+ |
LOC105376418 |
NNNTTACGTGGGGACCAAAA |
NGG |
2 |
0.2045 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
112387284 |
+ |
Ssu2 |
NNNTTACGTGGGGGAAAAAA |
NGG |
2 |
0.9412 |
Tier II |
| 2 |
NC_000072.6 |
6 |
56411051 |
- |
Pde1c |
NNNTTGGGTGGGGGGCAAAA |
NGG |
2 |
0.3361 |
Tier II |
Other clones with same target sequence:
(none)
