Construct: sgRNA BRDN0001146440

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGCATACCGCAGTACCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK4 (814)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77224

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 111449195 - CAMK4 NNNCATACCGCAGTACCCTG NGG 0 1.0 Tier I
2 NC_000011.10 11 66844159 - RCE1 NNNCATACCGGAGGACCCTG NGG 2 0.0 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 33158893 - Camk4 NNNCGTACCGCAGTACCCCG NGG 2 0.1986 Tier I
2 NC_000067.6 1 84893193 - Fbxo36 NNNCATACAACAGTACCCTG NGG 2 0.8 Tier II
3 NC_000072.6 6 53294360 - Creb5 NNNCACACCTCAGTACCCTG NGG 2 0.7386 Tier II
4 NC_000076.6 10 39513766 + Fyn NNNTACACCGCAGTACCCTG NGG 2 0.7273 Tier II
5 NC_000073.6 7 80276763 - Vps33b NNNCAGACCGCAGTACCCTC NGG 2 0.2449 Tier II
6 NC_000083.6 17 65265614 + Tmem232 NNNAATACCGCAGTACCCAG NGG 2 0.2406 Tier II
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Other clones with same target sequence:

(none)