Construct: sgRNA BRDN0001146443
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAAGGGTCAGGCATTAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKRA (8575)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76299
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
178444480 |
+ |
PRKRA |
NNNAAGGGTCAGGCATTAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
46375712 |
+ |
EPAS1 |
NNNAAGGGTCAGGTAGTAAG |
NGG |
2 |
0.2538 |
Tier I |
| 3 |
NC_000001.11 |
1 |
66224118 |
- |
PDE4B |
NNNAAGAGTCAGGCATCAAG |
NGG |
2 |
0.7059 |
Tier II |
| 4 |
NC_000012.12 |
12 |
100366419 |
- |
SLC17A8 |
NNNAAGGGTCAGGTAATAAG |
NGG |
2 |
0.6667 |
Tier II |
| 5 |
NC_000005.10 |
5 |
178728255 |
- |
ZNF354A |
NNNAAGGGGCAGGCATTAAA |
NGG |
2 |
0.5804 |
Tier II |
| 6 |
NC_000010.11 |
10 |
60339142 |
+ |
ANK3 |
NNNAAGGTTCAGACATTAAG |
NGG |
2 |
0.5769 |
Tier II |
| 7 |
NC_000001.11 |
1 |
27753309 |
+ |
FAM76A |
NNNAAGGGTAGGGCATTAAG |
NGG |
2 |
0.5633 |
Tier II |
| 8 |
NC_000017.11 |
17 |
19847947 |
- |
ULK2 |
NNNAAGGGTCACACATTAAG |
NGG |
2 |
0.4887 |
Tier II |
| 9 |
NC_000001.11 |
1 |
158003558 |
- |
KIRREL1 |
NNNGAGGGTCAGGCATCAAG |
NGG |
2 |
0.4412 |
Tier II |
| 10 |
NC_000011.10 |
11 |
16965473 |
- |
PLEKHA7 |
NNNAAGTGTCAGGCACTAAG |
NGG |
2 |
0.381 |
Tier II |
| 11 |
NC_000012.12 |
12 |
67274315 |
+ |
CAND1 |
NNNAATTGTCAGGCATTAAG |
NGG |
2 |
0.381 |
Tier II |
| 12 |
NC_000007.14 |
7 |
103382063 |
+ |
SLC26A5 |
NNNAAGGTTCAGGCATTTAG |
NGG |
2 |
0.3125 |
Tier II |
| 13 |
NC_000002.12 |
2 |
88109866 |
- |
SMYD1 |
NNNAAGGGCCAGGCATTCAG |
NGG |
2 |
0.1758 |
Tier II |
| 14 |
NC_000001.11 |
1 |
234206261 |
+ |
SLC35F3 |
NNNCTGGGTCAGGCATTAAG |
NGG |
2 |
0.1283 |
Tier II |
| 15 |
NC_000008.11 |
8 |
26591351 |
+ |
DPYSL2 |
NNNGAGGGTCAGGCATTCAG |
NGG |
2 |
0.119 |
Tier II |
| 16 |
NC_000002.12 |
2 |
130920396 |
+ |
ARHGEF4 |
NNNAAGGGCCAGGCATGAAG |
NGG |
2 |
0.1086 |
Tier II |
| 17 |
NC_000012.12 |
12 |
18521514 |
+ |
PIK3C2G |
NNNCAGGGTCAGGCATGAAG |
NGG |
2 |
0.0415 |
Tier II |
| 18 |
NC_000011.10 |
11 |
126951693 |
+ |
KIRREL3 |
NNNAAGTGTCAGGCATTAAG |
NGA |
2 |
0.0397 |
Tier II |
| 19 |
NC_000002.12 |
2 |
153360622 |
- |
GALNT13 |
NNNAAGGGTCAGGCCTGAAG |
NGG |
2 |
0.0321 |
Tier II |
| 20 |
NC_000015.10 |
15 |
78633913 |
+ |
CHRNB4 |
NNNAAGGGTCAGGGAATAAG |
NGG |
2 |
0.0 |
Tier II |
| 21 |
NC_000008.11 |
8 |
29767997 |
+ |
LINC02099 |
NNNAAGGGCCAAGCATTAAG |
NGG |
2 |
0.8615 |
Tier III |
| 22 |
NC_000006.12 |
6 |
43869078 |
+ |
LOC105375068 |
NNNAAGGGTCAGGAAATAAG |
NGG |
2 |
0.3182 |
Tier III |
| 23 |
NC_000006.12 |
6 |
43869078 |
+ |
LOC105375070 |
NNNAAGGGTCAGGAAATAAG |
NGG |
2 |
0.3182 |
Tier III |
| 24 |
NC_000024.10 |
Y |
21388826 |
+ |
PRORY |
NNNATGTGTCAGGCATTAAG |
NGG |
2 |
0.2078 |
Tier III |
| 25 |
NC_000008.11 |
8 |
26591351 |
+ |
LOC124901916 |
NNNGAGGGTCAGGCATTCAG |
NGG |
2 |
0.119 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
76640299 |
+ |
Prkra |
NNNATGGATCAGGCATTAAG |
NGG |
2 |
0.3636 |
Tier I |
| 2 |
NC_000081.6 |
15 |
76865030 |
- |
Zfp251 |
NNNAAGGATCAGGCATTAAA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000080.6 |
14 |
99892275 |
- |
Klf12 |
NNNAGGGGTCAAGCATTAAG |
NGG |
2 |
0.672 |
Tier II |
| 4 |
NC_000068.7 |
2 |
130503834 |
+ |
Ptpra |
NNNAAGGGTCCAGCATTAAG |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000071.6 |
5 |
71645114 |
+ |
Gabra4 |
NNNAAGAGTCAGGCATTAAG |
NAG |
2 |
0.2593 |
Tier II |
| 6 |
NC_000086.7 |
X |
105105789 |
- |
Pbdc1 |
NNNAGGGGTCAGGCAGTAAG |
NGG |
2 |
0.2492 |
Tier II |
| 7 |
NC_000076.6 |
10 |
78346338 |
- |
Agpat3 |
NNNAAGGGTCAGGCAGCAAG |
NGG |
2 |
0.2443 |
Tier II |
| 8 |
NC_000080.6 |
14 |
55599427 |
+ |
Rnf31 |
NNNGAGGGTCAGGAATTAAG |
NGG |
2 |
0.2188 |
Tier II |
| 9 |
NC_000080.6 |
14 |
115549179 |
+ |
Gpc5 |
NNNAAGGGTCAGACTTTAAG |
NGG |
2 |
0.1846 |
Tier II |
| 10 |
NC_000070.6 |
4 |
102393468 |
+ |
Pde4b |
NNNAAGGGTCAGGAATTGAG |
NGG |
2 |
0.14 |
Tier II |
| 11 |
NC_000083.6 |
17 |
83505094 |
+ |
Cox7a2l |
NNNAAGGGTCAGCCATTAAG |
NAG |
2 |
0.1092 |
Tier II |
| 12 |
NC_000083.6 |
17 |
56423877 |
+ |
Ptprs |
NNNAAGGGTCAGGCTTTATG |
NGG |
2 |
0.1077 |
Tier II |
| 13 |
NC_000067.6 |
1 |
20893577 |
- |
Paqr8 |
NNNAAGGGTCAGGCATTAGG |
NAG |
2 |
0.0972 |
Tier II |
| 14 |
NC_000080.6 |
14 |
31408657 |
+ |
Sh3bp5 |
NNNAAGGGTCAGCCATTAAG |
NGA |
2 |
0.0292 |
Tier II |
| 15 |
NC_000078.6 |
12 |
8325404 |
+ |
Hs1bp3 |
NNNAAGGGTCAGGCAGTAAG |
NGT |
2 |
0.0056 |
Tier II |
| 16 |
NC_000067.6 |
1 |
150105131 |
+ |
Ptgs2 |
NNNAAGGATCAGGGATTAAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000075.6 |
9 |
95445343 |
+ |
Gm32133 |
NNNAAGGTTCAGGCATTAAG |
NGC |
2 |
0.0139 |
Tier III |
Other clones with same target sequence:
(none)
