Construct: sgRNA BRDN0001146443
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAAGGGTCAGGCATTAAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PRKRA (8575)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000002.12 | 2 | 178444480 | + | PRKRA | NNNAAGGGTCAGGCATTAAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000002.12 | 2 | 46375712 | + | EPAS1 | NNNAAGGGTCAGGTAGTAAG | NGG | 2 | 0.2538 | Tier I |
3 | NC_000001.11 | 1 | 66224118 | - | PDE4B | NNNAAGAGTCAGGCATCAAG | NGG | 2 | 0.7059 | Tier II |
4 | NC_000012.12 | 12 | 100366419 | - | SLC17A8 | NNNAAGGGTCAGGTAATAAG | NGG | 2 | 0.6667 | Tier II |
5 | NC_000005.10 | 5 | 178728255 | - | ZNF354A | NNNAAGGGGCAGGCATTAAA | NGG | 2 | 0.5804 | Tier II |
6 | NC_000010.11 | 10 | 60339142 | + | ANK3 | NNNAAGGTTCAGACATTAAG | NGG | 2 | 0.5769 | Tier II |
7 | NC_000001.11 | 1 | 27753309 | + | FAM76A | NNNAAGGGTAGGGCATTAAG | NGG | 2 | 0.5633 | Tier II |
8 | NC_000017.11 | 17 | 19847947 | - | ULK2 | NNNAAGGGTCACACATTAAG | NGG | 2 | 0.4887 | Tier II |
9 | NC_000001.11 | 1 | 158003558 | - | KIRREL1 | NNNGAGGGTCAGGCATCAAG | NGG | 2 | 0.4412 | Tier II |
10 | NC_000011.10 | 11 | 16965473 | - | PLEKHA7 | NNNAAGTGTCAGGCACTAAG | NGG | 2 | 0.381 | Tier II |
11 | NC_000012.12 | 12 | 67274315 | + | CAND1 | NNNAATTGTCAGGCATTAAG | NGG | 2 | 0.381 | Tier II |
12 | NC_000006.12 | 6 | 43869078 | + | POLR1C | NNNAAGGGTCAGGAAATAAG | NGG | 2 | 0.3182 | Tier II |
13 | NC_000007.14 | 7 | 103382063 | + | SLC26A5 | NNNAAGGTTCAGGCATTTAG | NGG | 2 | 0.3125 | Tier II |
14 | NC_000002.12 | 2 | 88109866 | - | SMYD1 | NNNAAGGGCCAGGCATTCAG | NGG | 2 | 0.1758 | Tier II |
15 | NC_000001.11 | 1 | 234206261 | + | SLC35F3 | NNNCTGGGTCAGGCATTAAG | NGG | 2 | 0.1283 | Tier II |
16 | NC_000008.11 | 8 | 26591351 | + | DPYSL2 | NNNGAGGGTCAGGCATTCAG | NGG | 2 | 0.119 | Tier II |
17 | NC_000002.12 | 2 | 130920396 | + | ARHGEF4 | NNNAAGGGCCAGGCATGAAG | NGG | 2 | 0.1086 | Tier II |
18 | NC_000012.12 | 12 | 18521514 | + | PIK3C2G | NNNCAGGGTCAGGCATGAAG | NGG | 2 | 0.0415 | Tier II |
19 | NC_000011.10 | 11 | 126951693 | + | KIRREL3 | NNNAAGTGTCAGGCATTAAG | NGA | 2 | 0.0397 | Tier II |
20 | NC_000002.12 | 2 | 153360622 | - | GALNT13 | NNNAAGGGTCAGGCCTGAAG | NGG | 2 | 0.0321 | Tier II |
21 | NC_000015.10 | 15 | 78633913 | + | CHRNB4 | NNNAAGGGTCAGGGAATAAG | NGG | 2 | 0.0 | Tier II |
22 | NC_000008.11 | 8 | 29767997 | + | LINC02099 | NNNAAGGGCCAAGCATTAAG | NGG | 2 | 0.8615 | Tier III |
23 | NC_000006.12 | 6 | 43869078 | + | LOC105375068 | NNNAAGGGTCAGGAAATAAG | NGG | 2 | 0.3182 | Tier III |
24 | NC_000006.12 | 6 | 43869078 | + | LOC105375070 | NNNAAGGGTCAGGAAATAAG | NGG | 2 | 0.3182 | Tier III |
25 | NC_000024.10 | Y | 21388826 | + | PRORY | NNNATGTGTCAGGCATTAAG | NGG | 2 | 0.2078 | Tier III |
26 | NC_000008.11 | 8 | 26591351 | + | LOC124901916 | NNNGAGGGTCAGGCATTCAG | NGG | 2 | 0.119 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000068.7 | 2 | 76640299 | + | Prkra | NNNATGGATCAGGCATTAAG | NGG | 2 | 0.3636 | Tier I |
2 | NC_000081.6 | 15 | 76865030 | - | Zfp251 | NNNAAGGATCAGGCATTAAA | NGG | 2 | 0.9375 | Tier II |
3 | NC_000080.6 | 14 | 99892275 | - | Klf12 | NNNAGGGGTCAAGCATTAAG | NGG | 2 | 0.672 | Tier II |
4 | NC_000068.7 | 2 | 130503834 | + | Ptpra | NNNAAGGGTCCAGCATTAAG | NGG | 2 | 0.3733 | Tier II |
5 | NC_000071.6 | 5 | 71645114 | + | Gabra4 | NNNAAGAGTCAGGCATTAAG | NAG | 2 | 0.2593 | Tier II |
6 | NC_000086.7 | X | 105105789 | - | Pbdc1 | NNNAGGGGTCAGGCAGTAAG | NGG | 2 | 0.2492 | Tier II |
7 | NC_000076.6 | 10 | 78346338 | - | Agpat3 | NNNAAGGGTCAGGCAGCAAG | NGG | 2 | 0.2443 | Tier II |
8 | NC_000080.6 | 14 | 55599427 | + | Rnf31 | NNNGAGGGTCAGGAATTAAG | NGG | 2 | 0.2188 | Tier II |
9 | NC_000080.6 | 14 | 115549179 | + | Gpc5 | NNNAAGGGTCAGACTTTAAG | NGG | 2 | 0.1846 | Tier II |
10 | NC_000070.6 | 4 | 102393468 | + | Pde4b | NNNAAGGGTCAGGAATTGAG | NGG | 2 | 0.14 | Tier II |
11 | NC_000083.6 | 17 | 83505094 | + | Cox7a2l | NNNAAGGGTCAGCCATTAAG | NAG | 2 | 0.1092 | Tier II |
12 | NC_000083.6 | 17 | 56423877 | + | Ptprs | NNNAAGGGTCAGGCTTTATG | NGG | 2 | 0.1077 | Tier II |
13 | NC_000067.6 | 1 | 20893577 | - | Paqr8 | NNNAAGGGTCAGGCATTAGG | NAG | 2 | 0.0972 | Tier II |
14 | NC_000080.6 | 14 | 31408657 | + | Sh3bp5 | NNNAAGGGTCAGCCATTAAG | NGA | 2 | 0.0292 | Tier II |
15 | NC_000078.6 | 12 | 8325404 | + | Hs1bp3 | NNNAAGGGTCAGGCAGTAAG | NGT | 2 | 0.0056 | Tier II |
16 | NC_000067.6 | 1 | 150105131 | + | Ptgs2 | NNNAAGGATCAGGGATTAAG | NGG | 2 | 0.0 | Tier II |
17 | NC_000075.6 | 9 | 95445343 | + | Gm32133 | NNNAAGGTTCAGGCATTAAG | NGC | 2 | 0.0139 | Tier III |