Construct: sgRNA BRDN0001146450
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGCTCATAGTGAGAAACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STRADB (55437)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75494
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
201470023 |
- |
STRADB |
NNNCTCATAGTGAGAAACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
48436130 |
- |
SPATA6 |
NNNCTCGTAGTGAGAAACGT |
NGG |
1 |
0.7059 |
Tier II |
| 3 |
NC_000002.12 |
2 |
54499802 |
- |
SPTBN1 |
NNNTTCATAGTGAGAAACAT |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000012.12 |
12 |
64526035 |
- |
RASSF3 |
NNNCTCATAGTGAGAAATGA |
NGG |
2 |
0.3616 |
Tier II |
| 5 |
NC_000012.12 |
12 |
79664110 |
+ |
PAWR |
NNNGTCATAGTGAGAAATGT |
NGG |
2 |
0.3214 |
Tier II |
| 6 |
NC_000004.12 |
4 |
48654208 |
- |
FRYL |
NNNCTCTTAGTGAGAAAAGT |
NGG |
2 |
0.2356 |
Tier II |
| 7 |
NC_000018.10 |
18 |
63539080 |
- |
SERPINB12 |
NNNCTCATAGAGAGAAAGGT |
NGG |
2 |
0.1 |
Tier II |
| 8 |
NC_000017.11 |
17 |
79979718 |
- |
TBC1D16 |
NNNCTCCTAGTGAGAACCGT |
NGG |
2 |
0.0772 |
Tier II |
| 9 |
NC_000017.11 |
17 |
76622103 |
- |
ST6GALNAC1 |
NNNCTCATAGTGAGAAATGC |
NGG |
2 |
0.0584 |
Tier II |
| 10 |
NC_000004.12 |
4 |
113729323 |
- |
CAMK2D |
NNNCTCATGGTGAGAAACGT |
NGA |
2 |
0.0463 |
Tier II |
| 11 |
NC_000001.11 |
1 |
48436130 |
- |
LOC644462 |
NNNCTCGTAGTGAGAAACGT |
NGG |
1 |
0.7059 |
Tier III |
| 12 |
NC_000007.14 |
7 |
150513654 |
+ |
STRADBP1 |
NNNCTCGTAGTGAGAAACGT |
NGG |
1 |
0.7059 |
Tier III |
| 13 |
NC_000002.12 |
2 |
54499802 |
- |
C9orf85P1 |
NNNTTCATAGTGAGAAACAT |
NGG |
2 |
0.5714 |
Tier III |
| 14 |
NC_000003.12 |
3 |
144358936 |
- |
LOC105374140 |
NNNCTCGTAGTGAAAAACGT |
NGG |
2 |
0.5294 |
Tier III |
| 15 |
NC_000012.12 |
12 |
64526035 |
- |
LOC105369803 |
NNNCTCATAGTGAGAAATGA |
NGG |
2 |
0.3616 |
Tier III |
| 16 |
NC_000017.11 |
17 |
79979718 |
- |
LOC124904073 |
NNNCTCCTAGTGAGAACCGT |
NGG |
2 |
0.0772 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
118909157 |
+ |
Gli2 |
NNNCTCAGAGTGAGAAATGT |
NGG |
2 |
0.4714 |
Tier II |
| 2 |
NC_000077.6 |
11 |
30232327 |
+ |
Sptbn1 |
NNNCTCCTAGTGAGAAATGT |
NGG |
2 |
0.2812 |
Tier II |
| 3 |
NC_000073.6 |
7 |
58663852 |
- |
Atp10a |
NNNCTCATAGTTAGAAATGT |
NGG |
2 |
0.2473 |
Tier II |
| 4 |
NC_000069.6 |
3 |
28031708 |
+ |
Pld1 |
NNNCTGATAGTGAGAAGCGT |
NGG |
2 |
0.0882 |
Tier II |
| 5 |
NC_000077.6 |
11 |
47453412 |
+ |
Sgcd |
NNNCTCATAGTGAGAAAAGT |
NGT |
2 |
0.0087 |
Tier II |
| 6 |
NC_000077.6 |
11 |
30232327 |
+ |
Gm51887 |
NNNCTCCTAGTGAGAAATGT |
NGG |
2 |
0.2812 |
Tier III |
| 7 |
NC_000085.6 |
19 |
36534328 |
- |
Gm32143 |
NNNCTCATAATGAGAAACGC |
NGG |
2 |
0.0848 |
Tier III |
Other clones with same target sequence:
(none)
