Construct: sgRNA BRDN0001146451
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAACTAATGGTTAAACGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- COL4A3BP (10087)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77849
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
75416908 |
- |
CERT1 |
NNNACTAATGGTTAAACGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
99764472 |
+ |
CYP3A4 |
NNNACTAATGGTTAAATGTA |
NGG |
2 |
0.4375 |
Tier II |
| 3 |
NC_000005.10 |
5 |
122329904 |
+ |
SNCAIP |
NNNACTAACGGTTAAAAGTG |
NGG |
2 |
0.4308 |
Tier II |
| 4 |
NC_000015.10 |
15 |
54156542 |
- |
UNC13C |
NNNACTTATTGTTAAACGTG |
NGG |
2 |
0.3555 |
Tier II |
| 5 |
NC_000003.12 |
3 |
30741377 |
- |
GADL1 |
NNNACTACTGGTTAAATGTG |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000004.12 |
4 |
84779307 |
+ |
WDFY3 |
NNNACTAATGGTTAAACTTG |
NAG |
2 |
0.1728 |
Tier II |
| 7 |
NC_000013.11 |
13 |
31146668 |
+ |
HSPH1 |
NNNACTAATGGTTAAAAGTG |
NAG |
2 |
0.121 |
Tier II |
| 8 |
NC_000018.10 |
18 |
5571218 |
- |
EPB41L3 |
NNNACTAATGGTTCAAAGTG |
NGG |
2 |
0.1 |
Tier II |
| 9 |
NC_000006.12 |
6 |
7429821 |
- |
LOC102724234 |
NNNACTACAGGTTAAACGTG |
NGG |
2 |
0.398 |
Tier III |
| 10 |
NC_000013.11 |
13 |
48160003 |
- |
LOC105370198 |
NNNACTAATGGTTAAACTTC |
NGG |
2 |
0.2857 |
Tier III |
| 11 |
NC_000014.9 |
14 |
106584586 |
+ |
IGH |
NNNTCTAATGGTGAAACGTG |
NGG |
2 |
0.166 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
142469249 |
+ |
Lsp1 |
NNNACTGATGGATAAACGTG |
NGG |
2 |
0.5647 |
Tier II |
| 2 |
NC_000077.6 |
11 |
86745824 |
- |
Cltc |
NNNACTAATTGTGAAACGTG |
NGG |
2 |
0.212 |
Tier II |
| 3 |
NC_000078.6 |
12 |
57711815 |
- |
Ttc6 |
NNNACTAATGGTTAAAAGTG |
NGA |
2 |
0.0324 |
Tier II |
| 4 |
NC_000079.6 |
13 |
76644543 |
+ |
Mctp1 |
NNNACAAATGGTTAAACGTG |
NGT |
2 |
0.014 |
Tier II |
| 5 |
NC_000077.6 |
11 |
114776774 |
- |
Kif19a |
NNNACTAATGGTCAATCGTG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
