Construct: sgRNA BRDN0001146458

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGTACATGCCATAGAAGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA6 (285220)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76247

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 97226403 - EPHA6 NNNTACATGCCATAGAAGGT NGG 0 1.0 Tier I
2 NC_000012.12 12 120573145 + RNF10 NNNTACATGCCAAAGAAAGT NGG 2 0.4793 Tier II
3 NC_000016.10 16 83375228 + CDH13 NNNTACATTACATAGAAGGT NGG 2 0.4622 Tier II
4 NC_000002.12 2 7046066 + RNF144A NNNTACAGGGCATAGAAGGT NGG 2 0.2852 Tier II
5 NC_000010.11 10 118706890 + CACUL1 NNNTACATGCTACAGAAGGT NGG 2 0.2429 Tier II
6 NC_000017.11 17 43514819 + DHX8 NNNTTCATGCCATAGAATGT NGG 2 0.2424 Tier II
7 NC_000015.10 15 36748921 - CDIN1 NNNTACATGCCGGAGAAGGT NGG 2 0.1884 Tier II
8 NC_000011.10 11 19410699 + NAV2 NNNGACATGCCATAGGAGGT NGG 2 0.1202 Tier II
9 NC_000017.11 17 76634111 - ST6GALNAC1 NNNTCCATGCCATAGAAGGC NGG 2 0.0455 Tier II
10 NC_000005.10 5 176256450 - SIMC1 NNNAACATGCCATAGAAGGT NTG 2 0.0186 Tier II
11 NC_000023.11 X 76876555 + MIR325HG NNNTACATGCCAGAGAAGAT NGG 2 0.1863 Tier III
12 NC_000006.12 6 96219354 + UFL1-AS1 NNNAACATGGCATAGAAGGT NGG 2 0.1852 Tier III
13 NC_000003.12 3 67247427 - LOC112267879 NNNTACATGCCATAGATGTT NGG 2 0.0889 Tier III
14 NC_000014.9 14 38385988 + LOC124903304 NNNTACATGCCATAGAATGT NGC 2 0.0148 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 60220998 + Epha6 NNNTGCATGCCATAGAAGGT NGG 1 0.72 Tier I
2 NC_000078.6 12 112159526 + Kif26a NNNTACATGCCATAAAACGT NGG 2 0.4482 Tier II
3 NC_000067.6 1 140474164 - Kcnt2 NNNGACATGCCAAAGAAGGT NGG 2 0.4327 Tier II
4 NC_000069.6 3 68318787 + Schip1 NNNTACATGCCAAAGAAGGA NGG 2 0.3894 Tier II
5 NC_000069.6 3 68318787 + Iqschfp NNNTACATGCCAAAGAAGGA NGG 2 0.3894 Tier II
6 NC_000068.7 2 162111195 - Ptprt NNNTGCATTCCATAGAAGGT NGG 2 0.384 Tier II
7 NC_000069.6 3 109454177 - Vav3 NNNTTCATGCCAAAGAAGGT NGG 2 0.2517 Tier II
8 NC_000078.6 12 78732871 + Fam71d NNNTACATGCCTTTGAAGGT NGG 2 0.1778 Tier II
9 NC_000068.7 2 96952335 - Lrrc4c NNNTACATGCCATATAAAGT NGG 2 0.0989 Tier II
10 NC_000067.6 1 68327828 - Erbb4 NNNTAGATGCCATAGATGGT NGG 2 0.0667 Tier II
11 NC_000083.6 17 6553190 - Gm29721 NNNTACATGCCCTAGGAGGT NGG 2 0.0506 Tier II
12 NC_000083.6 17 6698228 + Sytl3 NNNTACATGCCCTAGGAGGT NGG 2 0.0506 Tier II
13 NC_000072.6 6 110871267 + Grm7 NNNTACATGCCATACATGGT NGG 2 0.0364 Tier II
14 NC_000077.6 11 9500584 + Abca13 NNNTACATGCCAAAGAAGGT NTG 2 0.027 Tier II
15 NC_000068.7 2 120861717 + Ubr1 NNNTACAAGCCATAGAAGGT NGC 2 0.0178 Tier II
16 NC_000074.6 8 118352978 + Cdh13 NNNTACCTGCCATAGAAGGT NGC 2 0.0097 Tier II
17 NC_000068.7 2 17648636 + Nebl NNNTACATGCCATAGCAGGT NTG 2 0.0 Tier II
18 NC_000075.6 9 57067930 + Gm10658 NNNTACATGCCATTGAGGGT NGG 2 0.0941 Tier III
19 NC_000083.6 17 6386310 - Gm29719 NNNTACATGCCCTAGGAGGT NGG 2 0.0506 Tier III
20 NC_000070.6 4 37284921 + Gm12374 NNNTACATGCCACAGAAGGT NTG 2 0.0308 Tier III
21 NC_000068.7 2 120861717 + AV039307 NNNTACAAGCCATAGAAGGT NGC 2 0.0178 Tier III
22 NC_000080.6 14 15442197 - B230110C06Rik NNNTACATGCTATAGAAGGT NTG 2 0.012 Tier III
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Other clones with same target sequence:

(none)