Construct: sgRNA BRDN0001146459

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGGGCCTGAGCATCGAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TYK2 (7297)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77246

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 10368383 + TYK2 NNNGGCCTGAGCATCGAAGA NGG 0 1.0 Tier I
2 NC_000019.10 19 35544952 - GAPDHS NNNGGCCTTAGCATCGAAGA NGG 1 0.5333 Tier I
3 NC_000011.10 11 17611363 + OTOG NNNGCCCTGAGCATCGTAGA NGG 2 0.1048 Tier I
4 NC_000012.12 12 30991332 + TSPAN11 NNNAGCCAGAGCATCGAAGA NGG 2 0.72 Tier II
5 NC_000013.11 13 30733962 - ALOX5AP NNNGGCCTGAGCATCAAGGA NGG 2 0.4 Tier II
6 NC_000005.10 5 148988653 - SH3TC2 NNNGGGCTGGGCATCGAAGA NGG 2 0.2778 Tier II
7 NC_000020.11 20 36309193 - DLGAP4 NNNGGCCTGGGCATCGAGGA NGG 2 0.2222 Tier II
8 NC_000016.10 16 10633442 + TEKT5 NNNAGCCTGAGCATCGAAGA NGC 2 0.02 Tier II
9 NC_000018.10 18 7677650 - PTPRM NNNGGCCTGATCATGGAAGA NGG 2 0.0192 Tier II
10 NC_000019.10 19 10631831 + SLC44A2 NNNGGCCTGATCATGGAAGA NGG 2 0.0192 Tier II
11 NC_000001.11 1 201695138 - NAV1 NNNGGCCTGAGCATGGAAGA NGC 2 0.0011 Tier II
12 NC_000001.11 1 15682807 + PLEKHM2 NNNGGCCTGAGCAGAGAAGA NGG 2 0.0 Tier II
13 NC_000012.12 12 30991332 + TSPAN11-AS1 NNNAGCCAGAGCATCGAAGA NGG 2 0.72 Tier III
14 NC_000019.10 19 35544952 - TMEM147-AS1 NNNGGCCTTAGCATCGAAGA NGG 1 0.5333 Tier III
15 NC_000013.11 13 30733962 - LOC124903146 NNNGGCCTGAGCATCAAGGA NGG 2 0.4 Tier III
16 NC_000020.11 20 58541152 + APCDD1L-DT NNNGGCCTGAGCACCGATGA NGG 2 0.1429 Tier III
17 NC_000021.9 21 8243037 - CDC27P9 NNNGGCCTGAGCCTCGAGGA NGG 2 0.0842 Tier III
18 NC_000021.9 21 8426061 - CDC27P10 NNNGGCCTGAGCCTCGAGGA NGG 2 0.0842 Tier III
19 NC_000021.9 21 8470622 - CDC27P11 NNNGGCCTGAGCCTCGAGGA NGG 2 0.0842 Tier III
20 NT_167214.1 142081 - LOC100286895 NNNGGCCTGAGCCTCGAGGA NGG 2 0.0842 Tier III
21 NT_187388.1 22 160065 - LOC102724621 NNNGGCCTGAGCCTCGAGGA NGG 2 0.0842 Tier III
22 NC_000015.10 15 25237221 + SNHG14 NNNGGCCTGAGCATAGCAGA NGG 2 0.0392 Tier III
23 NC_000001.11 1 201695138 - IPO9-AS1 NNNGGCCTGAGCATGGAAGA NGC 2 0.0011 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 16408124 - Top2b NNNGGCCAGAGCATCAAAGA NGG 2 0.8 Tier II
2 NC_000082.6 16 34092534 - Kalrn NNNAGGCTGAGCATCGAAGA NGG 2 0.45 Tier II
3 NC_000071.6 5 129148212 - Adgrd1 NNNAGCCTGAGCATCGAGGA NGG 2 0.36 Tier II
4 NC_000072.6 6 50130021 - Mpp6 NNNGGCCTGAGCATAGAAGA NAG 2 0.0576 Tier II
5 NC_000078.6 12 82349889 - Sipa1l1 NNNGGCCTGAGCTTGGAAGA NGG 2 0.015 Tier II
6 NC_000074.6 8 35535064 - Gm39162 NNNGGCCTGAGCATCGAAGG NTG 2 0.0298 Tier III
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Other clones with same target sequence:

(none)