Construct: sgRNA BRDN0001146460
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCGTCTACTCACAATATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK1G (57172)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75986
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
209607930 |
- |
CAMK1G |
NNNGTCTACTCACAATATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
12669388 |
- |
WDR83OS |
NNNGTCCACTAACAATATGT |
NGG |
2 |
0.5156 |
Tier I |
| 3 |
NC_000019.10 |
19 |
12669388 |
- |
WDR83 |
NNNGTCCACTAACAATATGT |
NGG |
2 |
0.5156 |
Tier II |
| 4 |
NC_000008.11 |
8 |
3043639 |
- |
CSMD1 |
NNNTTCTAATCACAATATGT |
NGG |
2 |
0.3117 |
Tier II |
| 5 |
NC_000004.12 |
4 |
163973804 |
- |
MARCHF1 |
NNNGGCTACTCAGAATATGT |
NGG |
2 |
0.0873 |
Tier II |
| 6 |
NC_000010.11 |
10 |
24235147 |
+ |
KIAA1217 |
NNNGTCTACTCACATTATGG |
NGG |
2 |
0.0353 |
Tier II |
| 7 |
NC_000003.12 |
3 |
30809105 |
+ |
GADL1 |
NNNGTCTACTCACAATATTT |
NTG |
2 |
0.026 |
Tier II |
| 8 |
NC_000009.12 |
9 |
89054293 |
- |
SHC3 |
NNNGTCTACTCACAATCTGC |
NGG |
2 |
0.016 |
Tier II |
| 9 |
NC_000002.12 |
2 |
146356823 |
+ |
LOC105373667 |
NNNTTCTGCTCACAATATGT |
NGG |
2 |
0.2667 |
Tier III |
| 10 |
NC_000003.12 |
3 |
30809105 |
+ |
FNBP1P2 |
NNNGTCTACTCACAATATTT |
NTG |
2 |
0.026 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
63138931 |
+ |
Chrna7 |
NNNGTCTATTCACAATATCT |
NGG |
2 |
0.3922 |
Tier II |
| 2 |
NC_000069.6 |
3 |
122002947 |
- |
Arhgap29 |
NNNGTATACTCAAAATATGT |
NGG |
2 |
0.3571 |
Tier II |
| 3 |
NC_000073.6 |
7 |
60121765 |
+ |
Snrpn |
NNNGTCTACTCTCAATATGA |
NGG |
2 |
0.1875 |
Tier II |
| 4 |
NC_000078.6 |
12 |
34361560 |
+ |
Hdac9 |
NNNGTCTACTCACAGTATGT |
NAG |
2 |
0.1685 |
Tier II |
| 5 |
NC_000073.6 |
7 |
47123490 |
- |
Ptpn5 |
NNNGTCAACTCACAATATGT |
NTG |
2 |
0.0341 |
Tier II |
| 6 |
NC_000068.7 |
2 |
49026430 |
- |
Mbd5 |
NNNCTCTACTCACAATATGT |
NTG |
2 |
0.0206 |
Tier II |
| 7 |
NC_000079.6 |
13 |
30821694 |
- |
Exoc2 |
NNNGGCTACTCACAATATGT |
NGC |
2 |
0.0142 |
Tier II |
| 8 |
NC_000073.6 |
7 |
60121765 |
+ |
Snhg14 |
NNNGTCTACTCTCAATATGA |
NGG |
2 |
0.1875 |
Tier III |
Other clones with same target sequence:
(none)
