Construct: sgRNA BRDN0001146461
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCCTGTGAAGCGAAGTCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R5 (23533)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75760
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
8887608 |
- |
PIK3R5 |
NNNCTGTGAAGCGAAGTCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
103190263 |
- |
CENPE |
NNNCTGTGAAGAAAAGTCAT |
NGG |
2 |
0.6593 |
Tier II |
| 3 |
NC_000003.12 |
3 |
68263577 |
- |
TAFA1 |
NNNCTGTAAAGCGAGGTCAT |
NGG |
2 |
0.65 |
Tier II |
| 4 |
NC_000001.11 |
1 |
233287023 |
+ |
PCNX2 |
NNNCTGTGATGTGAAGTCAT |
NGG |
2 |
0.4751 |
Tier II |
| 5 |
NC_000006.12 |
6 |
150994575 |
- |
MTHFD1L |
NNNCTGTGTAGAGAAGTCAT |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000001.11 |
1 |
41565300 |
+ |
HIVEP3 |
NNNCTGAGAAGCCAAGTCAT |
NGG |
2 |
0.3684 |
Tier II |
| 7 |
NC_000018.10 |
18 |
76436485 |
- |
ZNF516 |
NNNCTCTGAAGCCAAGTCAT |
NGG |
2 |
0.2871 |
Tier II |
| 8 |
NC_000006.12 |
6 |
162983716 |
- |
PACRG |
NNNCTGTGAAGCGAAAGCAT |
NGG |
2 |
0.1176 |
Tier II |
| 9 |
NC_000003.12 |
3 |
142621812 |
+ |
PLS1 |
NNNCTGTGAAGTGAAGTCCT |
NGG |
2 |
0.1114 |
Tier II |
| 10 |
NC_000022.11 |
22 |
45540836 |
+ |
FBLN1 |
NNNCTGTGAAGCCCAGTCAT |
NGG |
2 |
0.0902 |
Tier II |
| 11 |
NC_000007.14 |
7 |
158827891 |
+ |
ESYT2 |
NNNCTGTGAAGCCAAGTCAT |
NGC |
2 |
0.0094 |
Tier II |
| 12 |
NC_000002.12 |
2 |
81725259 |
- |
LOC102724542 |
NNNCTGTGAAGAAAAGTCAT |
NGG |
2 |
0.6593 |
Tier III |
| 13 |
NC_000005.10 |
5 |
79337276 |
+ |
LOC102724530 |
NNNCTGTGAAGAAAAGTCAT |
NGG |
2 |
0.6593 |
Tier III |
| 14 |
NC_000014.9 |
14 |
106244448 |
+ |
IGH |
NNNCTGTGAAGAAAAGTCAT |
NGG |
2 |
0.6593 |
Tier III |
| 15 |
NC_000003.12 |
3 |
68263577 |
- |
LOC107986019 |
NNNCTGTAAAGCGAGGTCAT |
NGG |
2 |
0.65 |
Tier III |
| 16 |
NC_000014.9 |
14 |
94460184 |
+ |
LOC105370635 |
NNNCTGTGAAGTGAAATCAT |
NGG |
2 |
0.5385 |
Tier III |
| 17 |
NC_000003.12 |
3 |
142621812 |
+ |
PLS1-AS1 |
NNNCTGTGAAGTGAAGTCCT |
NGG |
2 |
0.1114 |
Tier III |
| 18 |
NC_000012.12 |
12 |
75767073 |
+ |
LOC105369844 |
NNNCTGTGAAGCCAAGTCAG |
NGG |
2 |
0.0743 |
Tier III |
| 19 |
NC_000008.11 |
8 |
126960914 |
+ |
LOC105375751 |
NNNCTGTGAAGAGAAGTCAC |
NGG |
2 |
0.0649 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
34647856 |
+ |
Rars2 |
NNNCTGTGATGCGAAGTGAT |
NGG |
2 |
0.1176 |
Tier I |
| 2 |
NC_000067.6 |
1 |
140490770 |
- |
Kcnt2 |
NNNATGTGAAGCAAAGTCAT |
NGG |
2 |
0.7773 |
Tier II |
| 3 |
NC_000076.6 |
10 |
53374748 |
- |
Cep85l |
NNNCTGTGAAACGAAGTTAT |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000084.6 |
18 |
46297477 |
- |
Ccdc112 |
NNNCTGTGCAGCGAAGCCAT |
NGG |
2 |
0.4034 |
Tier II |
| 5 |
NC_000077.6 |
11 |
9271433 |
- |
Abca13 |
NNNCTGTGAAGAGAAGACAT |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000071.6 |
5 |
70789086 |
+ |
Gabrg1 |
NNNCTGAGAAGCCAAGTCAT |
NGG |
2 |
0.3684 |
Tier II |
| 7 |
NC_000069.6 |
3 |
72966711 |
- |
Sis |
NNNCAGTGAAGTGAAGTCAT |
NGG |
2 |
0.2692 |
Tier II |
| 8 |
NC_000078.6 |
12 |
57288067 |
- |
Mipol1 |
NNNCTGTTAAGCCAAGTCAT |
NGG |
2 |
0.2632 |
Tier II |
| 9 |
NC_000067.6 |
1 |
24062749 |
+ |
Fam135a |
NNNCTGGGAAGGGAAGTCAT |
NGG |
2 |
0.2614 |
Tier II |
| 10 |
NC_000072.6 |
6 |
104702505 |
+ |
Cntn6 |
NNNCTGTGAAGCTAAGTCAA |
NGG |
2 |
0.1687 |
Tier II |
| 11 |
NC_000068.7 |
2 |
141302960 |
+ |
Macrod2 |
NNNCTGTGAAGGGATGTCAT |
NGG |
2 |
0.0889 |
Tier II |
| 12 |
NC_000082.6 |
16 |
35070542 |
- |
Hacd2 |
NNNCTGTGAAGCTAAGGCAT |
NGG |
2 |
0.0353 |
Tier II |
| 13 |
NC_000067.6 |
1 |
174598439 |
+ |
Fmn2 |
NNNCTGTGAAGCGAAGTCAG |
NTG |
2 |
0.0069 |
Tier II |
| 14 |
NC_000075.6 |
9 |
113069558 |
+ |
Gm36251 |
NNNCTGTGAAGGGAAGTCAT |
NAG |
2 |
0.1152 |
Tier III |
Other clones with same target sequence:
(none)
