Construct: sgRNA BRDN0001146463

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTGGAGAAAAACTGGATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADK (132)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75742

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 74398545 + ADK NNNGGAGAAAAACTGGATGT NGG 0 1.0 Tier I
2 NC_000005.10 5 79734449 + CMYA5 NNNGGATGAAAACTGGATGT NGG 2 0.419 Tier I
3 NC_000023.11 X 77684320 + ATRX NNNGGAGAAAATCTGGATGT NTG 2 0.013 Tier I
4 NC_000023.11 X 17641824 + NHS NNNAAAGAAAAACTGGATGT NGG 2 0.78 Tier II
5 NC_000010.11 10 112227050 - GPAM NNNGGAGAAAAGCTGAATGT NGG 2 0.7222 Tier II
6 NC_000006.12 6 57099306 - ZNF451 NNNGGAAAAAAACTGGAAGT NGG 2 0.6667 Tier II
7 NC_000003.12 3 126785057 - CHCHD6 NNNGGAGAAAAACTGGATGA NGG 1 0.5625 Tier II
8 NC_000008.11 8 41834320 + ANK1 NNNGGAGAATAACAGGATGT NGG 2 0.5462 Tier II
9 NC_000001.11 1 171715108 + VAMP4 NNNGGAGATTAACTGGATGT NGG 2 0.5294 Tier II
10 NC_000004.12 4 118754634 + SEC24D NNNGGTGAAAAACTGGATTT NGG 2 0.4762 Tier II
11 NC_000016.10 16 1675218 - CRAMP1 NNNGGGGAAAGACTGGATGT NGG 2 0.4643 Tier II
12 NC_000015.10 15 58488887 + LIPC NNNGGAGAAAAACAGGATTT NGG 2 0.4127 Tier II
13 NC_000017.11 17 50140722 - PPP1R9B NNNGGAGAGAAACAGGATGT NGG 2 0.4127 Tier II
14 NC_000020.11 20 43169312 - PTPRT NNNGGAGAAAAAATAGATGT NGG 2 0.362 Tier II
15 NC_000005.10 5 97016690 - LNPEP NNNGGAGAACAACTAGATGT NGG 2 0.3137 Tier II
16 NC_000004.12 4 76370574 + CCDC158 NNNGGAGAAAAACTGGAGTT NGG 2 0.2222 Tier II
17 NC_000006.12 6 46430340 + RCAN2 NNNGTGGAAAAACTGGATGT NGG 2 0.2143 Tier II
18 NC_000001.11 1 40096203 - PPT1 NNNGGATAAAAACTGGAGGT NGG 2 0.1905 Tier II
19 NC_000012.12 12 7860168 + SLC2A14 NNNGGAGAAAAACTGGAAGT NAG 2 0.1728 Tier II
20 NC_000007.14 7 31736297 + PDE1C NNNAGAGAAAAACTGGCTGT NGG 2 0.1588 Tier II
21 NC_000016.10 16 5303535 + RBFOX1 NNNGGAGAAAAGCTGGCTGT NGG 2 0.1275 Tier II
22 NC_000004.12 4 182240118 + TENM3 NNNGGAGAAAAAGTGGATAT NGG 2 0.0974 Tier II
23 NC_000013.11 13 110315700 - COL4A2 NNNGGAGAAAAAGTGGATGA NGG 2 0.0767 Tier II
24 NC_000014.9 14 61069837 - SLC38A6 NNNGGAGGAAAACTGGATGC NGG 2 0.0667 Tier II
25 NC_000008.11 8 123890369 + FER1L6 NNNGGAGAAAAACTGGGGGT NGG 2 0.0588 Tier II
26 NC_000005.10 5 169923212 + DOCK2 NNNGTAGAAAAACTGGATGG NGG 2 0.0529 Tier II
27 NC_000005.10 5 169923212 + INSYN2B NNNGTAGAAAAACTGGATGG NGG 2 0.0529 Tier II
28 NC_000003.12 3 179516823 + GNB4 NNNGGAGAAAAACTGGCTGT NAG 2 0.0458 Tier II
29 NC_000007.14 7 141579922 + AGK NNNGGAGAAAAACTGGCTGT NAG 2 0.0458 Tier II
30 NC_000009.12 9 100142014 + INVS NNNGGAGAAAAACTGGCTGT NAG 2 0.0458 Tier II
31 NC_000015.10 15 74358261 - CYP11A1 NNNGGAGAAAAACTGGCTGT NAG 2 0.0458 Tier II
32 NC_000019.10 19 20091551 + ZNF90 NNNGGAGAAAAACTGGCTGT NAG 2 0.0458 Tier II
33 NC_000001.11 1 101854493 + OLFM3 NNNGGAGAAAAAATGGATGT NTG 2 0.015 Tier II
34 NC_000008.11 8 17279988 + VPS37A NNNGGAGAAAAACTCGATGT NTG 2 0.0106 Tier II
35 NC_000012.12 12 108848412 - SSH1 NNNGGAGAAAAACTGGAGGT NGC 2 0.0074 Tier II
36 NC_000003.12 3 124147683 - KALRN NNNGGAGAAAAACTGGATGG NTG 2 0.0069 Tier II
37 NC_000009.12 9 77925486 - GNAQ NNNGGAGAAAAACTGGGTGT NGT 2 0.0028 Tier II
38 NC_000015.10 15 65727039 + DENND4A NNNGGAGAAAAACTGGGTGT NGT 2 0.0028 Tier II
39 NC_000002.12 2 167346410 - B3GALT1 NNNGGAGAAAAACTTGATGT NGT 2 0.0023 Tier II
40 NC_000002.12 2 50930191 - NRXN1 NNNGGAGAAAAACTGGTTGT NGT 2 0.0022 Tier II
41 NC_000012.12 12 132037427 + EP400 NNNGGAGAAAAACGGGATGG NGG 2 0.0 Tier II
42 NC_000012.12 12 100784589 - ANO4 NNNGGAGAAAAACTGCATGT NGG 1 0.0 Tier II
43 NC_000010.11 10 25299364 + GPR158 NNNGGAGAAAAACTGTATGT NTG 2 0.0 Tier II
44 NC_000023.11 X 108731219 + IRS4 NNNGGAGAAAAAGGGGATGT NGG 2 0.0 Tier II
45 NC_000012.12 12 111841310 - MAPKAPK5-AS1 NNNAGAGAATAACTGGATGT NGG 2 0.7941 Tier III
46 NC_000023.11 X 17641824 + LOC105373142 NNNAAAGAAAAACTGGATGT NGG 2 0.78 Tier III
47 NC_000003.12 3 55320201 + LOC124906243 NNNAGAGAAAAACTGGAAGT NGG 2 0.6 Tier III
48 NC_000005.10 5 102582266 + LINC00492 NNNGAAGAAAGACTGGATGT NGG 2 0.5633 Tier III
49 NC_000007.14 7 39756920 + LINC00265 NNNGGAGGAAAACTGGAAGT NGG 2 0.4889 Tier III
50 NC_000015.10 15 58488887 + LIPC-AS1 NNNGGAGAAAAACAGGATTT NGG 2 0.4127 Tier III
51 NC_000018.10 18 39526740 + MIR924HG NNNGGAAAAAAACTGGAGGT NGG 2 0.3333 Tier III
52 NC_000009.12 9 38958087 + LOC124902157 NNNGGAGAAAAAATGGATTT NGG 2 0.2564 Tier III
53 NC_000009.12 9 67405854 - LOC101927602 NNNGGAGAAAAAATGGATTT NGG 2 0.2564 Tier III
54 NC_000013.11 13 111986141 - SOX1-OT NNNTGAGAAAAACAGGATGT NGG 2 0.2251 Tier III
55 NC_000011.10 11 45019731 - LOC105376650 NNNGGGGAAATACTGGATGT NGG 2 0.2198 Tier III
56 NC_000002.12 2 84329844 + LOC107985905 NNNGGAGTAAAACTGGAGGT NGG 2 0.1429 Tier III
57 NC_000005.10 5 17829096 + LINC02223 NNNAGAGAAAAACTTGATGT NGG 2 0.1286 Tier III
58 NC_000020.11 20 52692736 - LOC105372666 NNNGGAGAACAAATGGATGT NGG 2 0.1282 Tier III
59 NC_000003.12 3 44017148 - LOC124909489 NNNGGAGAAAGAGTGGATGT NGG 2 0.0886 Tier III
60 NC_000004.12 4 12476270 - LOC105374492 NNNGGAGAAAATCTGGATGT NAG 2 0.0864 Tier III
61 NC_000005.10 5 32897902 - LOC124900955 NNNGGAGCAAAACTGGATGG NGG 2 0.0756 Tier III
62 NC_000005.10 5 5046755 - LINC01020 NNNGGAAAAAAACTGGATGT NGA 2 0.0694 Tier III
63 NC_000005.10 5 128733753 - LOC105379168 NNNGGAGAAAAACTGGCTGT NAG 2 0.0458 Tier III
64 NC_000006.12 6 114013424 + HDAC2-AS2 NNNGGAGAAAAACTGGCTGT NAG 2 0.0458 Tier III
65 NC_000010.11 10 118898644 - LINC03036 NNNGGAGAAAAACTGGCTGT NAG 2 0.0458 Tier III
66 NC_000014.9 14 45496688 + LOC105370476 NNNGGAGAAAAACTGGCTGT NAG 2 0.0458 Tier III
67 NC_000005.10 5 151984866 + LINC01933 NNNGGAGAAAAACTGGTGGT NGG 2 0.0444 Tier III
68 NC_000012.12 12 68017536 + IFNG-AS1 NNNGGAGATAAACTGGATGT NGA 2 0.0417 Tier III
69 NC_000002.12 2 60735062 - ATP1B3P1 NNNGGGGAAAAACTGCATGT NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 11156251 + Zc3h7a NNNTGAGAAAAACTGGATGT NGG 1 0.3636 Tier I
2 NC_000070.6 4 140739176 - Padi6 NNNGCAGAAAAAATGGATGT NGG 2 0.3022 Tier I
3 NC_000078.6 12 51441456 + Scfd1 NNNAGAGAAAAACTGGAAGT NGG 2 0.6 Tier II
4 NC_000074.6 8 78618948 - Slc10a7 NNNGCTGAAAAACTGGATGT NGG 2 0.5612 Tier II
5 NC_000081.6 15 18861736 - Cdh10 NNNGGAAAAGAACTGGATGT NGG 2 0.5556 Tier II
6 NC_000069.6 3 26853404 + Spata16 NNNGGAGAATAACAGGATGT NGG 2 0.5462 Tier II
7 NC_000072.6 6 126858198 - Akap3 NNNGGAGAAAAACTGAATCT NGG 2 0.4483 Tier II
8 NC_000078.6 12 66664504 - Mdga2 NNNGGAGGAGAACTGGATGT NGG 2 0.4074 Tier II
9 NC_000085.6 19 53842813 + Rbm20 NNNGGAGCAAAATTGGATGT NGG 2 0.3 Tier II
10 NC_000079.6 13 88985673 - Edil3 NNNGGAGAAAAAATGGATAT NGG 2 0.2747 Tier II
11 NC_000078.6 12 117181053 + Ptprn2 NNNGGAGAAACACTGGAAGT NGG 2 0.2667 Tier II
12 NC_000077.6 11 3209329 - Eif4enif1 NNNGGATTAAAACTGGATGT NGG 2 0.2449 Tier II
13 NC_000069.6 3 50394899 + Slc7a11 NNNGCAGAAATACTGGATGT NGG 2 0.2418 Tier II
14 NC_000071.6 5 115525847 + Pxn NNNGGAGACAAACTGGAGGT NGG 2 0.1905 Tier II
15 NC_000070.6 4 43630478 + Npr2 NNNGGAGAGAAACTGGCTGT NGG 2 0.1176 Tier II
16 NC_000070.6 4 126809379 + AU040320 NNNGGAGAAAACATGGATGT NGG 2 0.1012 Tier II
17 NC_000069.6 3 88697642 - Khdc4 NNNGGGGAAAAAGTGGATGT NGG 2 0.0974 Tier II
18 NC_000082.6 16 70436375 + Gbe1 NNNGGAGAAAAAATGGCTGT NGG 2 0.0679 Tier II
19 NC_000076.6 10 57731253 - Pkib NNNTGAGAAAAACTGGATGG NGG 2 0.0642 Tier II
20 NC_000078.6 12 58989774 - Sec23a NNNGGAGAAAATCTGGCTGT NGG 2 0.0588 Tier II
21 NC_000075.6 9 104302791 + Acpp NNNGGAGAGAAACTGGATGT NGA 2 0.0463 Tier II
22 NC_000075.6 9 58587973 + Nptn NNNGGAGAAAAGCTGGATGT NTG 2 0.0281 Tier II
23 NC_000072.6 6 100256362 + Rybp NNNTGAGAAAAACTGGATGT NGA 2 0.0253 Tier II
24 NC_000072.6 6 121455006 - Iqsec3 NNNGGAAAAAAACTGGATGT NGC 2 0.0222 Tier II
25 NC_000081.6 15 54601312 + Mal2 NNNGGAGCAAAACTGGATGT NGT 2 0.0069 Tier II
26 NC_000076.6 10 10778069 - Grm1 NNNGGAGAAAAACTGGGTGT NGT 2 0.0028 Tier II
27 NC_000082.6 16 64815155 + Zfp654 NNNGGAGAAAAACTGTATGT NGA 2 0.0 Tier II
28 NC_000078.6 12 98211685 + Galc NNNGGAGAAAAACTGTATTT NGG 2 0.0 Tier II
29 NC_000072.6 6 46674104 + Cntnap2 NNNGGAGAAAAACTGTCTGT NGG 2 0.0 Tier II
30 NC_000069.6 3 66264470 - Veph1 NNNGGAGAAAAATGGGATGT NGG 2 0.0 Tier II
31 NC_000079.6 13 51098125 + Spin1 NNNGGAGAAAAATTGTATGT NGG 2 0.0 Tier II
32 NC_000080.6 14 69470373 + Gm21451 NNNGGAGAAAGACTGCATGT NGG 2 0.0 Tier II
33 NC_000080.6 14 69470373 + Gm16867 NNNGGAGAAAGACTGCATGT NGG 2 0.0 Tier II
34 NC_000080.6 14 69688648 + Loxl2 NNNGGAGAAAGACTGCATGT NGG 2 0.0 Tier II
35 NC_000069.6 3 18628701 + Gm30789 NNNGGAGAAAAACAGAATGT NGG 2 0.619 Tier III
36 NC_000069.6 3 26853404 + Gm32458 NNNGGAGAATAACAGGATGT NGG 2 0.5462 Tier III
37 NC_000067.6 1 132288206 - Gm38446 NNNGGAGAAAAACTGGAAGG NGG 2 0.1176 Tier III
38 NC_000074.6 8 115862395 + Gm31037 NNNGGAGAGAAACTGGCTGT NGG 2 0.1176 Tier III
39 NC_000079.6 13 51098125 + 9430083A17Rik NNNGGAGAAAAATTGTATGT NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)