Construct: sgRNA BRDN0001146463
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGGAGAAAAACTGGATGT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ADK (132)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000010.11 | 10 | 74398545 | + | ADK | NNNGGAGAAAAACTGGATGT | NGG | 0 | 1.0 | Tier I |
2 | NC_000005.10 | 5 | 79734449 | + | CMYA5 | NNNGGATGAAAACTGGATGT | NGG | 2 | 0.419 | Tier I |
3 | NC_000023.11 | X | 77684320 | + | ATRX | NNNGGAGAAAATCTGGATGT | NTG | 2 | 0.013 | Tier I |
4 | NC_000023.11 | X | 17641824 | + | NHS | NNNAAAGAAAAACTGGATGT | NGG | 2 | 0.78 | Tier II |
5 | NC_000010.11 | 10 | 112227050 | - | GPAM | NNNGGAGAAAAGCTGAATGT | NGG | 2 | 0.7222 | Tier II |
6 | NC_000006.12 | 6 | 57099306 | - | ZNF451 | NNNGGAAAAAAACTGGAAGT | NGG | 2 | 0.6667 | Tier II |
7 | NC_000003.12 | 3 | 126785057 | - | CHCHD6 | NNNGGAGAAAAACTGGATGA | NGG | 1 | 0.5625 | Tier II |
8 | NC_000008.11 | 8 | 41834320 | + | ANK1 | NNNGGAGAATAACAGGATGT | NGG | 2 | 0.5462 | Tier II |
9 | NC_000001.11 | 1 | 171715108 | + | VAMP4 | NNNGGAGATTAACTGGATGT | NGG | 2 | 0.5294 | Tier II |
10 | NC_000004.12 | 4 | 118754634 | + | SEC24D | NNNGGTGAAAAACTGGATTT | NGG | 2 | 0.4762 | Tier II |
11 | NC_000016.10 | 16 | 1675218 | - | CRAMP1 | NNNGGGGAAAGACTGGATGT | NGG | 2 | 0.4643 | Tier II |
12 | NC_000015.10 | 15 | 58488887 | + | LIPC | NNNGGAGAAAAACAGGATTT | NGG | 2 | 0.4127 | Tier II |
13 | NC_000017.11 | 17 | 50140722 | - | PPP1R9B | NNNGGAGAGAAACAGGATGT | NGG | 2 | 0.4127 | Tier II |
14 | NC_000020.11 | 20 | 43169312 | - | PTPRT | NNNGGAGAAAAAATAGATGT | NGG | 2 | 0.362 | Tier II |
15 | NC_000005.10 | 5 | 97016690 | - | LNPEP | NNNGGAGAACAACTAGATGT | NGG | 2 | 0.3137 | Tier II |
16 | NC_000004.12 | 4 | 76370574 | + | CCDC158 | NNNGGAGAAAAACTGGAGTT | NGG | 2 | 0.2222 | Tier II |
17 | NC_000006.12 | 6 | 46430340 | + | RCAN2 | NNNGTGGAAAAACTGGATGT | NGG | 2 | 0.2143 | Tier II |
18 | NC_000001.11 | 1 | 40096203 | - | PPT1 | NNNGGATAAAAACTGGAGGT | NGG | 2 | 0.1905 | Tier II |
19 | NC_000012.12 | 12 | 7860168 | + | SLC2A14 | NNNGGAGAAAAACTGGAAGT | NAG | 2 | 0.1728 | Tier II |
20 | NC_000007.14 | 7 | 31736297 | + | PDE1C | NNNAGAGAAAAACTGGCTGT | NGG | 2 | 0.1588 | Tier II |
21 | NC_000016.10 | 16 | 5303535 | + | RBFOX1 | NNNGGAGAAAAGCTGGCTGT | NGG | 2 | 0.1275 | Tier II |
22 | NC_000004.12 | 4 | 182240118 | + | TENM3 | NNNGGAGAAAAAGTGGATAT | NGG | 2 | 0.0974 | Tier II |
23 | NC_000013.11 | 13 | 110315700 | - | COL4A2 | NNNGGAGAAAAAGTGGATGA | NGG | 2 | 0.0767 | Tier II |
24 | NC_000014.9 | 14 | 61069837 | - | SLC38A6 | NNNGGAGGAAAACTGGATGC | NGG | 2 | 0.0667 | Tier II |
25 | NC_000008.11 | 8 | 123890369 | + | FER1L6 | NNNGGAGAAAAACTGGGGGT | NGG | 2 | 0.0588 | Tier II |
26 | NC_000005.10 | 5 | 169923212 | + | DOCK2 | NNNGTAGAAAAACTGGATGG | NGG | 2 | 0.0529 | Tier II |
27 | NC_000005.10 | 5 | 169923212 | + | INSYN2B | NNNGTAGAAAAACTGGATGG | NGG | 2 | 0.0529 | Tier II |
28 | NC_000003.12 | 3 | 179516823 | + | GNB4 | NNNGGAGAAAAACTGGCTGT | NAG | 2 | 0.0458 | Tier II |
29 | NC_000007.14 | 7 | 141579922 | + | AGK | NNNGGAGAAAAACTGGCTGT | NAG | 2 | 0.0458 | Tier II |
30 | NC_000009.12 | 9 | 100142014 | + | INVS | NNNGGAGAAAAACTGGCTGT | NAG | 2 | 0.0458 | Tier II |
31 | NC_000015.10 | 15 | 74358261 | - | CYP11A1 | NNNGGAGAAAAACTGGCTGT | NAG | 2 | 0.0458 | Tier II |
32 | NC_000019.10 | 19 | 20091551 | + | ZNF90 | NNNGGAGAAAAACTGGCTGT | NAG | 2 | 0.0458 | Tier II |
33 | NC_000001.11 | 1 | 101854493 | + | OLFM3 | NNNGGAGAAAAAATGGATGT | NTG | 2 | 0.015 | Tier II |
34 | NC_000008.11 | 8 | 17279988 | + | VPS37A | NNNGGAGAAAAACTCGATGT | NTG | 2 | 0.0106 | Tier II |
35 | NC_000012.12 | 12 | 108848412 | - | SSH1 | NNNGGAGAAAAACTGGAGGT | NGC | 2 | 0.0074 | Tier II |
36 | NC_000003.12 | 3 | 124147683 | - | KALRN | NNNGGAGAAAAACTGGATGG | NTG | 2 | 0.0069 | Tier II |
37 | NC_000009.12 | 9 | 77925486 | - | GNAQ | NNNGGAGAAAAACTGGGTGT | NGT | 2 | 0.0028 | Tier II |
38 | NC_000015.10 | 15 | 65727039 | + | DENND4A | NNNGGAGAAAAACTGGGTGT | NGT | 2 | 0.0028 | Tier II |
39 | NC_000002.12 | 2 | 167346410 | - | B3GALT1 | NNNGGAGAAAAACTTGATGT | NGT | 2 | 0.0023 | Tier II |
40 | NC_000002.12 | 2 | 50930191 | - | NRXN1 | NNNGGAGAAAAACTGGTTGT | NGT | 2 | 0.0022 | Tier II |
41 | NC_000012.12 | 12 | 132037427 | + | EP400 | NNNGGAGAAAAACGGGATGG | NGG | 2 | 0.0 | Tier II |
42 | NC_000012.12 | 12 | 100784589 | - | ANO4 | NNNGGAGAAAAACTGCATGT | NGG | 1 | 0.0 | Tier II |
43 | NC_000010.11 | 10 | 25299364 | + | GPR158 | NNNGGAGAAAAACTGTATGT | NTG | 2 | 0.0 | Tier II |
44 | NC_000023.11 | X | 108731219 | + | IRS4 | NNNGGAGAAAAAGGGGATGT | NGG | 2 | 0.0 | Tier II |
45 | NC_000012.12 | 12 | 111841310 | - | MAPKAPK5-AS1 | NNNAGAGAATAACTGGATGT | NGG | 2 | 0.7941 | Tier III |
46 | NC_000023.11 | X | 17641824 | + | LOC105373142 | NNNAAAGAAAAACTGGATGT | NGG | 2 | 0.78 | Tier III |
47 | NC_000003.12 | 3 | 55320201 | + | LOC124906243 | NNNAGAGAAAAACTGGAAGT | NGG | 2 | 0.6 | Tier III |
48 | NC_000005.10 | 5 | 102582266 | + | LINC00492 | NNNGAAGAAAGACTGGATGT | NGG | 2 | 0.5633 | Tier III |
49 | NC_000007.14 | 7 | 39756920 | + | LINC00265 | NNNGGAGGAAAACTGGAAGT | NGG | 2 | 0.4889 | Tier III |
50 | NC_000015.10 | 15 | 58488887 | + | LIPC-AS1 | NNNGGAGAAAAACAGGATTT | NGG | 2 | 0.4127 | Tier III |
51 | NC_000018.10 | 18 | 39526740 | + | MIR924HG | NNNGGAAAAAAACTGGAGGT | NGG | 2 | 0.3333 | Tier III |
52 | NC_000009.12 | 9 | 38958087 | + | LOC124902157 | NNNGGAGAAAAAATGGATTT | NGG | 2 | 0.2564 | Tier III |
53 | NC_000009.12 | 9 | 67405854 | - | LOC101927602 | NNNGGAGAAAAAATGGATTT | NGG | 2 | 0.2564 | Tier III |
54 | NC_000013.11 | 13 | 111986141 | - | SOX1-OT | NNNTGAGAAAAACAGGATGT | NGG | 2 | 0.2251 | Tier III |
55 | NC_000011.10 | 11 | 45019731 | - | LOC105376650 | NNNGGGGAAATACTGGATGT | NGG | 2 | 0.2198 | Tier III |
56 | NC_000002.12 | 2 | 84329844 | + | LOC107985905 | NNNGGAGTAAAACTGGAGGT | NGG | 2 | 0.1429 | Tier III |
57 | NC_000005.10 | 5 | 17829096 | + | LINC02223 | NNNAGAGAAAAACTTGATGT | NGG | 2 | 0.1286 | Tier III |
58 | NC_000020.11 | 20 | 52692736 | - | LOC105372666 | NNNGGAGAACAAATGGATGT | NGG | 2 | 0.1282 | Tier III |
59 | NC_000003.12 | 3 | 44017148 | - | LOC124909489 | NNNGGAGAAAGAGTGGATGT | NGG | 2 | 0.0886 | Tier III |
60 | NC_000004.12 | 4 | 12476270 | - | LOC105374492 | NNNGGAGAAAATCTGGATGT | NAG | 2 | 0.0864 | Tier III |
61 | NC_000005.10 | 5 | 32897902 | - | LOC124900955 | NNNGGAGCAAAACTGGATGG | NGG | 2 | 0.0756 | Tier III |
62 | NC_000005.10 | 5 | 5046755 | - | LINC01020 | NNNGGAAAAAAACTGGATGT | NGA | 2 | 0.0694 | Tier III |
63 | NC_000005.10 | 5 | 128733753 | - | LOC105379168 | NNNGGAGAAAAACTGGCTGT | NAG | 2 | 0.0458 | Tier III |
64 | NC_000006.12 | 6 | 114013424 | + | HDAC2-AS2 | NNNGGAGAAAAACTGGCTGT | NAG | 2 | 0.0458 | Tier III |
65 | NC_000010.11 | 10 | 118898644 | - | LINC03036 | NNNGGAGAAAAACTGGCTGT | NAG | 2 | 0.0458 | Tier III |
66 | NC_000014.9 | 14 | 45496688 | + | LOC105370476 | NNNGGAGAAAAACTGGCTGT | NAG | 2 | 0.0458 | Tier III |
67 | NC_000005.10 | 5 | 151984866 | + | LINC01933 | NNNGGAGAAAAACTGGTGGT | NGG | 2 | 0.0444 | Tier III |
68 | NC_000012.12 | 12 | 68017536 | + | IFNG-AS1 | NNNGGAGATAAACTGGATGT | NGA | 2 | 0.0417 | Tier III |
69 | NC_000002.12 | 2 | 60735062 | - | ATP1B3P1 | NNNGGGGAAAAACTGCATGT | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000082.6 | 16 | 11156251 | + | Zc3h7a | NNNTGAGAAAAACTGGATGT | NGG | 1 | 0.3636 | Tier I |
2 | NC_000070.6 | 4 | 140739176 | - | Padi6 | NNNGCAGAAAAAATGGATGT | NGG | 2 | 0.3022 | Tier I |
3 | NC_000078.6 | 12 | 51441456 | + | Scfd1 | NNNAGAGAAAAACTGGAAGT | NGG | 2 | 0.6 | Tier II |
4 | NC_000074.6 | 8 | 78618948 | - | Slc10a7 | NNNGCTGAAAAACTGGATGT | NGG | 2 | 0.5612 | Tier II |
5 | NC_000081.6 | 15 | 18861736 | - | Cdh10 | NNNGGAAAAGAACTGGATGT | NGG | 2 | 0.5556 | Tier II |
6 | NC_000069.6 | 3 | 26853404 | + | Spata16 | NNNGGAGAATAACAGGATGT | NGG | 2 | 0.5462 | Tier II |
7 | NC_000072.6 | 6 | 126858198 | - | Akap3 | NNNGGAGAAAAACTGAATCT | NGG | 2 | 0.4483 | Tier II |
8 | NC_000078.6 | 12 | 66664504 | - | Mdga2 | NNNGGAGGAGAACTGGATGT | NGG | 2 | 0.4074 | Tier II |
9 | NC_000085.6 | 19 | 53842813 | + | Rbm20 | NNNGGAGCAAAATTGGATGT | NGG | 2 | 0.3 | Tier II |
10 | NC_000079.6 | 13 | 88985673 | - | Edil3 | NNNGGAGAAAAAATGGATAT | NGG | 2 | 0.2747 | Tier II |
11 | NC_000078.6 | 12 | 117181053 | + | Ptprn2 | NNNGGAGAAACACTGGAAGT | NGG | 2 | 0.2667 | Tier II |
12 | NC_000077.6 | 11 | 3209329 | - | Eif4enif1 | NNNGGATTAAAACTGGATGT | NGG | 2 | 0.2449 | Tier II |
13 | NC_000069.6 | 3 | 50394899 | + | Slc7a11 | NNNGCAGAAATACTGGATGT | NGG | 2 | 0.2418 | Tier II |
14 | NC_000071.6 | 5 | 115525847 | + | Pxn | NNNGGAGACAAACTGGAGGT | NGG | 2 | 0.1905 | Tier II |
15 | NC_000070.6 | 4 | 43630478 | + | Npr2 | NNNGGAGAGAAACTGGCTGT | NGG | 2 | 0.1176 | Tier II |
16 | NC_000070.6 | 4 | 126809379 | + | AU040320 | NNNGGAGAAAACATGGATGT | NGG | 2 | 0.1012 | Tier II |
17 | NC_000069.6 | 3 | 88697642 | - | Khdc4 | NNNGGGGAAAAAGTGGATGT | NGG | 2 | 0.0974 | Tier II |
18 | NC_000082.6 | 16 | 70436375 | + | Gbe1 | NNNGGAGAAAAAATGGCTGT | NGG | 2 | 0.0679 | Tier II |
19 | NC_000076.6 | 10 | 57731253 | - | Pkib | NNNTGAGAAAAACTGGATGG | NGG | 2 | 0.0642 | Tier II |
20 | NC_000078.6 | 12 | 58989774 | - | Sec23a | NNNGGAGAAAATCTGGCTGT | NGG | 2 | 0.0588 | Tier II |
21 | NC_000075.6 | 9 | 104302791 | + | Acpp | NNNGGAGAGAAACTGGATGT | NGA | 2 | 0.0463 | Tier II |
22 | NC_000075.6 | 9 | 58587973 | + | Nptn | NNNGGAGAAAAGCTGGATGT | NTG | 2 | 0.0281 | Tier II |
23 | NC_000072.6 | 6 | 100256362 | + | Rybp | NNNTGAGAAAAACTGGATGT | NGA | 2 | 0.0253 | Tier II |
24 | NC_000072.6 | 6 | 121455006 | - | Iqsec3 | NNNGGAAAAAAACTGGATGT | NGC | 2 | 0.0222 | Tier II |
25 | NC_000081.6 | 15 | 54601312 | + | Mal2 | NNNGGAGCAAAACTGGATGT | NGT | 2 | 0.0069 | Tier II |
26 | NC_000076.6 | 10 | 10778069 | - | Grm1 | NNNGGAGAAAAACTGGGTGT | NGT | 2 | 0.0028 | Tier II |
27 | NC_000082.6 | 16 | 64815155 | + | Zfp654 | NNNGGAGAAAAACTGTATGT | NGA | 2 | 0.0 | Tier II |
28 | NC_000078.6 | 12 | 98211685 | + | Galc | NNNGGAGAAAAACTGTATTT | NGG | 2 | 0.0 | Tier II |
29 | NC_000072.6 | 6 | 46674104 | + | Cntnap2 | NNNGGAGAAAAACTGTCTGT | NGG | 2 | 0.0 | Tier II |
30 | NC_000069.6 | 3 | 66264470 | - | Veph1 | NNNGGAGAAAAATGGGATGT | NGG | 2 | 0.0 | Tier II |
31 | NC_000079.6 | 13 | 51098125 | + | Spin1 | NNNGGAGAAAAATTGTATGT | NGG | 2 | 0.0 | Tier II |
32 | NC_000080.6 | 14 | 69470373 | + | Gm21451 | NNNGGAGAAAGACTGCATGT | NGG | 2 | 0.0 | Tier II |
33 | NC_000080.6 | 14 | 69470373 | + | Gm16867 | NNNGGAGAAAGACTGCATGT | NGG | 2 | 0.0 | Tier II |
34 | NC_000080.6 | 14 | 69688648 | + | Loxl2 | NNNGGAGAAAGACTGCATGT | NGG | 2 | 0.0 | Tier II |
35 | NC_000069.6 | 3 | 18628701 | + | Gm30789 | NNNGGAGAAAAACAGAATGT | NGG | 2 | 0.619 | Tier III |
36 | NC_000069.6 | 3 | 26853404 | + | Gm32458 | NNNGGAGAATAACAGGATGT | NGG | 2 | 0.5462 | Tier III |
37 | NC_000067.6 | 1 | 132288206 | - | Gm38446 | NNNGGAGAAAAACTGGAAGG | NGG | 2 | 0.1176 | Tier III |
38 | NC_000074.6 | 8 | 115862395 | + | Gm31037 | NNNGGAGAGAAACTGGCTGT | NGG | 2 | 0.1176 | Tier III |
39 | NC_000079.6 | 13 | 51098125 | + | 9430083A17Rik | NNNGGAGAAAAATTGTATGT | NGG | 2 | 0.0 | Tier III |