Construct: sgRNA BRDN0001146469
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTGGCCAAGAAACACTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK4 (440275)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75876
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
39943478 |
+ |
EIF2AK4 |
NNNGGCCAAGAAACACTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
97804316 |
+ |
TMEM131 |
NNNGCCAAAGAAACACTGTG |
NGG |
2 |
0.5893 |
Tier II |
| 3 |
NC_000011.10 |
11 |
46476745 |
+ |
AMBRA1 |
NNNGACCAAGAAACACTTTG |
NGG |
2 |
0.5778 |
Tier II |
| 4 |
NC_000012.12 |
12 |
45802720 |
+ |
ARID2 |
NNNCACCAAGAAACACTGTG |
NGG |
2 |
0.4588 |
Tier II |
| 5 |
NC_000017.11 |
17 |
75144994 |
+ |
JPT1 |
NNNCACCAAGAAACACTGTG |
NGG |
2 |
0.4588 |
Tier II |
| 6 |
NC_000003.12 |
3 |
126741787 |
- |
CHCHD6 |
NNNGGCCAAAAAACACTCTG |
NGG |
2 |
0.4444 |
Tier II |
| 7 |
NC_000001.11 |
1 |
21517570 |
- |
ALPL |
NNNGGCTAAGAAACACTGTC |
NGG |
2 |
0.3482 |
Tier II |
| 8 |
NC_000001.11 |
1 |
36980962 |
+ |
GRIK3 |
NNNGGCCAAGAAACAATGAG |
NGG |
2 |
0.2857 |
Tier II |
| 9 |
NC_000009.12 |
9 |
544523 |
+ |
KANK1 |
NNNGGCCAACAAACACTATG |
NGG |
2 |
0.2769 |
Tier II |
| 10 |
NC_000017.11 |
17 |
48175858 |
+ |
SKAP1 |
NNNGGCCAAGCAGCACTGTG |
NGG |
2 |
0.2609 |
Tier II |
| 11 |
NC_000016.10 |
16 |
3980333 |
+ |
ADCY9 |
NNNGGCCAAGAACCACTGTG |
NGG |
1 |
0.2105 |
Tier II |
| 12 |
NC_000010.11 |
10 |
76909548 |
- |
KCNMA1 |
NNNGGCAAAGAAACCCTGTG |
NGG |
2 |
0.2045 |
Tier II |
| 13 |
NC_000011.10 |
11 |
9820430 |
+ |
SBF2 |
NNNGGCCAAGGCACACTGTG |
NGG |
2 |
0.1711 |
Tier II |
| 14 |
NC_000001.11 |
1 |
236550222 |
- |
LGALS8 |
NNNGGCCCAGAAACACTGGG |
NGG |
2 |
0.1071 |
Tier II |
| 15 |
NC_000001.11 |
1 |
236550222 |
- |
HEATR1 |
NNNGGCCCAGAAACACTGGG |
NGG |
2 |
0.1071 |
Tier II |
| 16 |
NC_000017.11 |
17 |
31144601 |
- |
NF1 |
NNNGGCCAAGAAACACTGAG |
NAG |
2 |
0.0741 |
Tier II |
| 17 |
NC_000017.11 |
17 |
54995855 |
- |
STXBP4 |
NNNGGCCAAGAAACAGTCTG |
NGG |
2 |
0.0733 |
Tier II |
| 18 |
NC_000015.10 |
15 |
27743611 |
+ |
OCA2 |
NNNGGCCCAGAAACACGGTG |
NGG |
2 |
0.0504 |
Tier II |
| 19 |
NC_000001.11 |
1 |
67046789 |
+ |
SLC35D1 |
NNNGGCCAGGAAACACTGTG |
NTG |
2 |
0.026 |
Tier II |
| 20 |
NC_000017.11 |
17 |
41504990 |
- |
KRT13 |
NNNGGCCAAGAAACACAGTG |
NTG |
2 |
0.0208 |
Tier II |
| 21 |
NC_000010.11 |
10 |
48832388 |
- |
WDFY4 |
NNNGGCTAAGAAACACTGTG |
NGC |
2 |
0.0181 |
Tier II |
| 22 |
NC_000005.10 |
5 |
102993379 |
- |
PAM |
NNNGGCCAAGAAACACTTTG |
NGT |
2 |
0.0108 |
Tier II |
| 23 |
NC_000012.12 |
12 |
80930708 |
- |
LIN7A |
NNNGGCCAAGAAAGAATGTG |
NGG |
2 |
0.0 |
Tier II |
| 24 |
NC_000011.10 |
11 |
88507281 |
+ |
GRM5 |
NNNGGCTAAGAAAGACTGTG |
NGG |
2 |
0.0 |
Tier II |
| 25 |
NC_000019.10 |
19 |
16052484 |
+ |
OR1AB1P |
NNNGGCCAATGAACACTGTG |
NGG |
2 |
0.5281 |
Tier III |
| 26 |
NC_000014.9 |
14 |
95334188 |
+ |
LINC02292 |
NNNGACCAAGAAACACAGTG |
NGG |
2 |
0.4622 |
Tier III |
| 27 |
NC_000017.11 |
17 |
75144994 |
+ |
LOC107985034 |
NNNCACCAAGAAACACTGTG |
NGG |
2 |
0.4588 |
Tier III |
| 28 |
NC_000014.9 |
14 |
22518443 |
+ |
TRA |
NNNAGCCCAGAAACACTGTG |
NGG |
2 |
0.3857 |
Tier III |
| 29 |
NC_000014.9 |
14 |
22518443 |
+ |
TRAJ26 |
NNNAGCCCAGAAACACTGTG |
NGG |
2 |
0.3857 |
Tier III |
| 30 |
NC_000010.11 |
10 |
76909548 |
- |
KCNMA1-AS1 |
NNNGGCAAAGAAACCCTGTG |
NGG |
2 |
0.2045 |
Tier III |
| 31 |
NC_000010.11 |
10 |
48832388 |
- |
LOC105378299 |
NNNGGCTAAGAAACACTGTG |
NGC |
2 |
0.0181 |
Tier III |
| 32 |
NC_000011.10 |
11 |
88507281 |
+ |
GRM5-AS1 |
NNNGGCTAAGAAAGACTGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
21674523 |
+ |
Smarca4 |
NNNGGTCAAGGAACACTGTG |
NGG |
2 |
0.6036 |
Tier II |
| 2 |
NC_000085.6 |
19 |
3966152 |
- |
Aldh3b3 |
NNNGACCAAGAAACACAGTG |
NGG |
2 |
0.4622 |
Tier II |
| 3 |
NC_000078.6 |
12 |
75944865 |
- |
Syne2 |
NNNGGCCAAGAAACAATGTC |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000077.6 |
11 |
51444937 |
- |
Col23a1 |
NNNGGCCATGAAGCACTGTG |
NGG |
2 |
0.3913 |
Tier II |
| 5 |
NC_000077.6 |
11 |
47950002 |
+ |
Sgcd |
NNNGACCTAGAAACACTGTG |
NGG |
2 |
0.3714 |
Tier II |
| 6 |
NC_000067.6 |
1 |
134552963 |
- |
Mgat4e |
NNNGACCAACAAACACTGTG |
NGG |
2 |
0.3467 |
Tier II |
| 7 |
NC_000080.6 |
14 |
11954375 |
- |
Ptprg |
NNNGGCCAAGAATCACTGTA |
NGG |
2 |
0.2813 |
Tier II |
| 8 |
NC_000070.6 |
4 |
127024393 |
- |
Sfpq |
NNNGGCAAAGAAACCCTGTG |
NGG |
2 |
0.2045 |
Tier II |
| 9 |
NC_000069.6 |
3 |
87936545 |
+ |
Isg20l2 |
NNNGGCAAAGACACACTGTG |
NGG |
2 |
0.1974 |
Tier II |
| 10 |
NC_000084.6 |
18 |
20315465 |
- |
Dsg1a |
NNNGGCCAAGAGACACTGTG |
NAG |
2 |
0.1872 |
Tier II |
| 11 |
NC_000067.6 |
1 |
159547871 |
+ |
Tnr |
NNNGGCCAAGAAACACGTTG |
NGG |
2 |
0.0784 |
Tier II |
| 12 |
NC_000078.6 |
12 |
57584254 |
- |
Ttc6 |
NNNGGCCAAGAAACACTATG |
NGT |
2 |
0.0112 |
Tier II |
| 13 |
NC_000080.6 |
14 |
54206550 |
+ |
Tcra |
NNNGACTAAGAAACACTGTG |
NGG |
2 |
0.7042 |
Tier III |
| 14 |
NC_000080.6 |
14 |
54206550 |
+ |
Traj12 |
NNNGACTAAGAAACACTGTG |
NGG |
2 |
0.7042 |
Tier III |
| 15 |
NC_000076.6 |
10 |
17541165 |
+ |
Gm32543 |
NNNGGCCAGGAAATACTGTG |
NGG |
2 |
0.4889 |
Tier III |
| 16 |
NC_000074.6 |
8 |
15041496 |
- |
BB014433 |
NNNGGCATAGAAACACTGTG |
NGG |
2 |
0.3214 |
Tier III |
| 17 |
NC_000072.6 |
6 |
86487908 |
- |
2310040G24Rik |
NNNGGCCAAGAATCACTATG |
NGG |
2 |
0.2077 |
Tier III |
| 18 |
NC_000074.6 |
8 |
102174048 |
- |
Gm51539 |
NNNGGCCATGTAACACTGTG |
NGG |
2 |
0.1846 |
Tier III |
| 19 |
NC_000070.6 |
4 |
89484818 |
- |
Gm12610 |
NNNGGCCAAAAAACACTGTG |
NGA |
2 |
0.0648 |
Tier III |
| 20 |
NC_000069.6 |
3 |
52953181 |
+ |
Gm30351 |
NNNAGCCAAGAAAGACTGTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
