Construct: sgRNA BRDN0001146475

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATGGCATTATATTTCCCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1G1 (53944)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77441

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 64216676 + CSNK1G1 NNNGCATTATATTTCCCACA NGG 0 1.0 Tier I
2 NC_000005.10 5 123573428 - CSNK1G3 NNNGCATTGTATTTACCACA NGG 2 0.1481 Tier I
3 NC_000007.14 7 11446251 + THSD7A NNNGCATTATATTTGTCACA NGG 2 0.0154 Tier I
4 NC_000023.11 X 136213538 - MAP7D3 NNNGCATTATGTCTCCCACA NGG 2 0.5132 Tier II
5 NC_000012.12 12 23909162 + SOX5 NNNGCATTATATTTCAAACA NGG 2 0.4667 Tier II
6 NC_000001.11 1 15713581 - PLEKHM2 NNNGAATTATAATTCCCACA NGG 2 0.4571 Tier II
7 NC_000007.14 7 147269400 - CNTNAP2 NNNGCATTATATATCCAACA NGG 2 0.3231 Tier II
8 NC_000013.11 13 41849412 + VWA8 NNNTCAATATATTTCCCACA NGG 2 0.3182 Tier II
9 NC_000005.10 5 42568255 - GHR NNNTCATTAAATTTCCCACA NGG 2 0.3117 Tier II
10 NC_000004.12 4 71269714 - SLC4A4 NNNGCATTATATTTCTCACA NGG 1 0.3077 Tier II
11 NC_000015.10 15 32098054 - CHRNA7 NNNGAATTATAGTTCCCACA NGG 2 0.2857 Tier II
12 NC_000008.11 8 3490228 + CSMD1 NNNGCATTATGTTTCCCAAA NGG 2 0.2786 Tier II
13 NC_000013.11 13 36294521 + CCDC169 NNNGCATTTTATTTCCCATA NGG 2 0.2769 Tier II
14 NC_000013.11 13 36294521 + CCDC169-SOHLH2 NNNGCATTTTATTTCCCATA NGG 2 0.2769 Tier II
15 NC_000003.12 3 53130084 - RFT1 NNNGCATTCTATTTCCCATA NGG 2 0.2637 Tier II
16 NC_000006.12 6 53098263 + FBXO9 NNNGCATTTTATTTCCCGCA NGG 2 0.24 Tier II
17 NC_000011.10 11 40455178 - LRRC4C NNNGCATTATATTTCCCTTA NGG 2 0.2308 Tier II
18 NC_000002.12 2 30574482 - LCLAT1 NNNTCATTATATTTCCCATA NGG 2 0.1678 Tier II
19 NC_000008.11 8 123257341 - ZHX1 NNNGGATTATATGTCCCACA NGG 2 0.1565 Tier II
20 NC_000008.11 8 123257341 - ZHX1-C8orf76 NNNGGATTATATGTCCCACA NGG 2 0.1565 Tier II
21 NC_000012.12 12 815019 - WNK1 NNNGCATTAGATTTCTCACA NGG 2 0.1538 Tier II
22 NC_000019.10 19 53521014 + ZNF331 NNNGCATTACATTTCCCAGA NGG 2 0.0667 Tier II
23 NC_000006.12 6 142309330 - ADGRG6 NNNGCATTTTATTTGCCACA NGG 2 0.03 Tier II
24 NC_000014.9 14 71459169 + SIPA1L1 NNNGCATTATATTTCTCACA NGA 2 0.0214 Tier II
25 NC_000004.12 4 23173037 - LOC105374524 NNNGAATTATAATTCCCACA NGG 2 0.4571 Tier III
26 NC_000006.12 6 104349426 - LOC102724443 NNNGCATTATTTTTCTCACA NGG 2 0.0947 Tier III
27 NC_000008.11 8 71931492 - MSC-AS1 NNNGCATTATACTTCCCACA NGA 2 0.0658 Tier III
28 NC_000011.10 11 109757341 + LINC02715 NNNGCATAATATTTCCCACA NTG 2 0.0312 Tier III
29 NC_000002.12 2 19031742 - LOC105373456 NNNGGATTATATTTCCCACA NGT 2 0.0097 Tier III
30 NC_000002.12 2 208631069 + LOC101927960 NNNTCATTATATTTCCCACA NGT 2 0.0059 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 65999433 - Csnk1g1 NNNGCATTGTACTTCCCACA NGG 2 0.6316 Tier I
2 NC_000075.6 9 20314157 + Olfr18 NNNGCTTTATATTTCCCACC NGG 2 0.1623 Tier I
3 NC_000075.6 9 30958940 - Adamts8 NNNGCATTATATTTCTCACA NTG 2 0.012 Tier I
4 NC_000073.6 7 122529571 - Prkcb NNNGCACCATATTTCCCACA NGG 2 0.6875 Tier II
5 NC_000068.7 2 33168624 - Ralgps1 NNNGCATTCCATTTCCCACA NGG 2 0.3048 Tier II
6 NC_000084.6 18 43266742 + Stk32a NNNTCATTATAATTCCCACA NGG 2 0.2909 Tier II
7 NC_000081.6 15 3454137 + Ghr NNNCCATTACATTTCCCACA NGG 2 0.2824 Tier II
8 NC_000072.6 6 9048104 + Nxph1 NNNGCAGTATTTTTCCCACA NGG 2 0.181 Tier II
9 NC_000072.6 6 92875420 - Adamts9 NNNGCATTTTATTTCCCACA NAG 2 0.1556 Tier II
10 NC_000075.6 9 80364377 - Impg1 NNNGCATTATATTTCCCCCT NGG 2 0.1143 Tier II
11 NC_000069.6 3 55635668 + Nbea NNNGCAATATATTTTCCACA NGG 2 0.0583 Tier II
12 NC_000077.6 11 21831227 - Wdpcp NNNCCATTATATTTCCCACA NGA 2 0.0368 Tier II
13 NC_000069.6 3 68924074 + Ift80 NNNGCATTATATTTCCCAAA NGA 2 0.0298 Tier II
14 NC_000076.6 10 83167924 - Chst11 NNNGCATTATGTTTCCCACA NTG 2 0.0253 Tier II
15 NC_000070.6 4 48155858 - Stx17 NNNGCAGTATATTTCCCACA NTG 2 0.0229 Tier II
16 NC_000067.6 1 188480223 + Ush2a NNNGCATTATATTTCACACA NGC 2 0.0222 Tier II
17 NC_000070.6 4 83295482 - Ttc39b NNNGCATTATATTTCTCACA NTG 2 0.012 Tier II
18 NC_000073.6 7 61220645 + A230006K03Rik NNNGCATTATATTACACACA NGG 2 0.619 Tier III
19 NC_000081.6 15 32166187 + Gm32499 NNNGAATTATATTTCACACA NGG 2 0.5714 Tier III
20 NC_000068.7 2 3405973 - Gm39750 NNNGCAATCTATTTCCCACA NGG 2 0.5 Tier III
21 NC_000068.7 2 144887229 - Gm39952 NNNGCATTAAATTTCCCATA NGG 2 0.3956 Tier III
22 NC_000072.6 6 92875420 - Gm15737 NNNGCATTTTATTTCCCACA NAG 2 0.1556 Tier III
23 NC_000070.6 4 23819842 - Gm31145 NNNGCATTATATTTTCCACA NAG 2 0.0173 Tier III
24 NC_000075.6 9 113106238 - Gm36251 NNNGCATTATATTTTCCCCA NGG 2 0.0127 Tier III
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Other clones with same target sequence:

(none)