Construct: sgRNA BRDN0001146478
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGACTGATGAGGGCGAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK9 (1025)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77169
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
127788653 |
- |
CDK9 |
NNNGACTGATGAGGGCGAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
41028002 |
- |
SCMH1 |
NNNGACTGAGGAGGGAGAGT |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000005.10 |
5 |
139374023 |
- |
SLC23A1 |
NNNTACTGATGAGGGAGAGT |
NGG |
2 |
0.3636 |
Tier II |
| 4 |
NC_000013.11 |
13 |
67152997 |
- |
PCDH9 |
NNNGAGTGATGAGGGCTAGT |
NGG |
2 |
0.125 |
Tier II |
| 5 |
NC_000003.12 |
3 |
10529154 |
+ |
ATP2B2 |
NNNGACTGATGAGAGGGAGT |
NGG |
2 |
0.1154 |
Tier II |
| 6 |
NC_000019.10 |
19 |
13957425 |
+ |
DCAF15 |
NNNGACTGCTGAGGGGGAGT |
NGG |
2 |
0.0879 |
Tier II |
| 7 |
NC_000001.11 |
1 |
17097063 |
+ |
PADI2 |
NNNGACGGATGAGGTCGAGT |
NGG |
2 |
0.084 |
Tier II |
| 8 |
NC_000014.9 |
14 |
64831130 |
- |
SPTB |
NNNGACTGAGGAGGGGGAGT |
NGG |
2 |
0.0769 |
Tier II |
| 9 |
NC_000016.10 |
16 |
73809668 |
- |
ZFHX3 |
NNNGACTGATGAGGGTGAGT |
NGC |
2 |
0.0068 |
Tier II |
| 10 |
NC_000001.11 |
1 |
41028002 |
- |
SLFNL1-AS1 |
NNNGACTGAGGAGGGAGAGT |
NGG |
2 |
0.5 |
Tier III |
| 11 |
NC_000016.10 |
16 |
48628952 |
- |
LOC105371240 |
NNNGTCTGATGAGGGAGAGT |
NGG |
2 |
0.3636 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
66415878 |
+ |
Map2 |
NNNAACTGATGAGGGGGAGT |
NGG |
2 |
0.1385 |
Tier I |
| 2 |
NC_000068.7 |
2 |
32709507 |
+ |
Cdk9 |
NNNGGCTGATGAGGGCGAGC |
NGG |
2 |
0.0655 |
Tier I |
| 3 |
NC_000069.6 |
3 |
87725612 |
- |
Arhgef11 |
NNNGACTGATGAAGGCGAGT |
NGC |
2 |
0.0205 |
Tier I |
| 4 |
NC_000073.6 |
7 |
98260853 |
- |
Acer3 |
NNNGAGTGATGAGGGCAAGT |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000086.7 |
X |
50862205 |
- |
Stk26 |
NNNGTCTGATGAGGGAGAGT |
NGG |
2 |
0.3636 |
Tier II |
| 6 |
NC_000074.6 |
8 |
125825087 |
+ |
Pcnx2 |
NNNGACTGATGTGGGAGAGT |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000067.6 |
1 |
19103639 |
+ |
Tfap2d |
NNNGACTGATGAGGGACAGT |
NGG |
2 |
0.2353 |
Tier II |
| 8 |
NC_000071.6 |
5 |
135445082 |
+ |
Hip1 |
NNNGACTGATGAGGGCAAGT |
NCG |
2 |
0.1 |
Tier II |
| 9 |
NC_000075.6 |
9 |
14901436 |
- |
Izumo1r |
NNNGTCTGATGAGGGCCAGT |
NGG |
2 |
0.0856 |
Tier II |
| 10 |
NC_000070.6 |
4 |
156214201 |
- |
Perm1 |
NNNGGCTGATGAGGGCGAGC |
NGG |
2 |
0.0655 |
Tier II |
| 11 |
NC_000082.6 |
16 |
65825846 |
- |
Vgll3 |
NNNGACTGATGAGGGCGTGT |
NTG |
2 |
0.0195 |
Tier II |
| 12 |
NC_000069.6 |
3 |
137988961 |
- |
Gm19708 |
NNNGACAGATGAGAGCGAGT |
NGG |
2 |
0.6563 |
Tier III |
| 13 |
NC_000067.6 |
1 |
19103639 |
+ |
Gm15825 |
NNNGACTGATGAGGGACAGT |
NGG |
2 |
0.2353 |
Tier III |
| 14 |
NC_000077.6 |
11 |
77644993 |
- |
Gm12733 |
NNNGACTGATGAGGGCCATT |
NGG |
2 |
0.1569 |
Tier III |
| 15 |
NC_000070.6 |
4 |
156214201 |
- |
Gm46877 |
NNNGGCTGATGAGGGCGAGC |
NGG |
2 |
0.0655 |
Tier III |
Other clones with same target sequence:
(none)
