Construct: sgRNA BRDN0001146480

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAAGTGCTAGTCGAAAACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX16 (64089)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75835

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 81823920 + SNX16 NNNAGTGCTAGTCGAAAACC NGG 0 1.0 Tier I
2 NC_000017.11 17 18002192 - DRC3 NNNAGTGCTAGCAGAAAACC NGG 2 0.3644 Tier II
3 NC_000002.12 2 31124939 - GALNT14 NNNAGTGCTAGTAGAAGACC NGG 2 0.0679 Tier II
4 NC_000007.14 7 123781877 + HYAL4 NNNAGTGCTAGTCTAGAACC NGG 2 0.0513 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 10434486 + Snx16 NNNAGTGCTAATCGAAAGCC NGG 2 0.4 Tier I
2 NC_000074.6 8 118894404 - Cdh13 NNNAGTGCTAATCGAACACC NGG 2 0.1765 Tier II
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Other clones with same target sequence:

(none)