Construct: sgRNA BRDN0001146482

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCCAGATAAATGTAAAATCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGK (55750)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76155

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 141601243 - AGK NNNAGATAAATGTAAAATCG NGG 0 1.0 Tier I
2 NC_000003.12 3 85902588 - CADM2 NNNAGATAAATGTAAAATCA NGG 1 0.9375 Tier II
3 NC_000004.12 4 42142335 + BEND4 NNNAGATAAAAGTAAAATCA NGG 2 0.7031 Tier II
4 NC_000013.11 13 77126823 - MYCBP2 NNNAGATAAAAGTAAAATCA NGG 2 0.7031 Tier II
5 NC_000012.12 12 104002552 - GLT8D2 NNNAGATAATTGTAAAATCT NGG 2 0.6176 Tier II
6 NC_000007.14 7 24798329 - OSBPL3 NNNGGATAAATGTAGAATCG NGG 2 0.4063 Tier II
7 NC_000014.9 14 52565542 - GPR137C NNNAAATAAATGTAAAATAG NGG 2 0.3714 Tier II
8 NC_000005.10 5 77330157 - PDE8B NNNAGATAAATGTAAAAAAG NGG 2 0.2857 Tier II
9 NC_000001.11 1 150811394 + ARNT NNNAGACAAAGGTAAAATCG NGG 2 0.275 Tier II
10 NC_000003.12 3 33184348 - SUSD5 NNNAGATAAATTTAAAATCT NGG 2 0.2692 Tier II
11 NC_000010.11 10 112662076 - VTI1A NNNAGATAAATTTAAAATCT NGG 2 0.2692 Tier II
12 NC_000016.10 16 85429821 - GSE1 NNNAGAAAAATGGAAAATCG NGG 2 0.2283 Tier II
13 NC_000002.12 2 162172270 - FAP NNNAGCTAAATGTAAAATCC NGG 2 0.1948 Tier II
14 NC_000013.11 13 21503481 + MICU2 NNNAGATAAATTTAAAATTG NGG 2 0.1775 Tier II
15 NC_000003.12 3 157361012 - VEPH1 NNNAGATAAAGGTAAAATAG NGG 2 0.1714 Tier II
16 NC_000006.12 6 6598334 - LY86 NNNAGATAAATGTAAACTCA NGG 2 0.1654 Tier II
17 NC_000011.10 11 84142414 + DLG2 NNNAGATAAATTTAAAATCC NGG 2 0.1648 Tier II
18 NC_000004.12 4 142381068 - INPP4B NNNAGATATATGGAAAATCG NGG 2 0.1565 Tier II
19 NC_000003.12 3 267422 - CHL1 NNNATATAAATGTAAAATAG NGG 2 0.1286 Tier II
20 NC_000002.12 2 204704678 - PARD3B NNNAGATAAATGTAAAATCT NTG 2 0.0273 Tier II
21 NC_000002.12 2 205818009 - LOC105373847 NNNAGATAAATGTAAAATCA NGG 1 0.9375 Tier III
22 NC_000023.11 X 132131094 + AGKP2 NNNGGATAAATGTAAAATCG NGG 1 0.625 Tier III
23 NC_000002.12 2 34312526 - LOC105374457 NNNAAATAAATGTAAAATCC NGG 2 0.3714 Tier III
24 NC_000002.12 2 80927068 - LOC105374827 NNNAGATTAATGTAAAATCT NGG 2 0.3 Tier III
25 NC_000013.11 13 111422876 + LOC107984618 NNNAGAGAAATGTAAAATTG NGG 2 0.2715 Tier III
26 NC_000002.12 2 162172270 - LOC101929532 NNNAGCTAAATGTAAAATCC NGG 2 0.1948 Tier III
27 NC_000003.12 3 157361012 - LOC101928236 NNNAGATAAAGGTAAAATAG NGG 2 0.1714 Tier III
28 NC_000006.12 6 6598334 - LY86-AS1 NNNAGATAAATGTAAACTCA NGG 2 0.1654 Tier III
29 NC_000008.11 8 127830506 - PVT1 NNNAGATAAATGTAAAATCT NCG 2 0.075 Tier III
30 NC_000011.10 11 39707839 - LOC105376637 NNNAGATAAGTGTAAAATGG NGG 2 0.0694 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 107023847 + Acss3 NNNGTATAAATGTAAAATCG NGG 2 0.1875 Tier I
2 NC_000067.6 1 110923833 - Cdh19 NNNAAATAAATATAAAATCG NGG 2 0.8089 Tier II
3 NC_000086.7 X 95930089 - Las1l NNNAGACAGATGTAAAATCG NGG 2 0.4583 Tier II
4 NC_000086.7 X 95930089 - Zc3h12b NNNAGACAGATGTAAAATCG NGG 2 0.4583 Tier II
5 NC_000072.6 6 66926840 - Gng12 NNNAGATAAATGTGAAATCA NGG 2 0.4375 Tier II
6 NC_000072.6 6 92523306 + Prickle2 NNNAACTAAATGTAAAATCG NGG 2 0.3939 Tier II
7 NC_000075.6 9 28121957 + Opcml NNNAGATAAATCTAAAATCT NGG 2 0.3706 Tier II
8 NC_000085.6 19 57812300 - Atrnl1 NNNGGATACATGTAAAATCG NGG 2 0.3571 Tier II
9 NC_000069.6 3 146516541 + Rpf1 NNNAGATAAATGAAAAATAG NGG 2 0.2967 Tier II
10 NC_000067.6 1 3212440 - Xkr4 NNNGGATAAATGTAAAATAG NGG 2 0.2679 Tier II
11 NC_000068.7 2 38906950 + Nr6a1 NNNAGATAAATGTAAAAGCT NGG 2 0.2333 Tier II
12 NC_000068.7 2 60860042 + Rbms1 NNNAGATAAAGGTAAAACCG NGG 2 0.1714 Tier II
13 NC_000084.6 18 7456226 - Mpp7 NNNAGATGAATGTATAATCG NGG 2 0.1467 Tier II
14 NC_000074.6 8 38426190 - Sgcz NNNAGATAAATGTAAAATGA NGG 2 0.1172 Tier II
15 NC_000084.6 18 75442632 + Ctif NNNAGATAAATGTCAAAGCG NGG 2 0.0714 Tier II
16 NC_000080.6 14 100097558 - Klf12 NNNAGATAAATGTAAATTCC NGG 2 0.0571 Tier II
17 NC_000078.6 12 57994757 + Gm46329 NNNAGATAGATGTAAAATCC NGG 2 0.2857 Tier III
18 NC_000068.7 2 38906950 + Nr6a1os NNNAGATAAATGTAAAAGCT NGG 2 0.2333 Tier III
19 NC_000081.6 15 40120242 - Gm33251 NNNCGATAAATGTAAAATCG NAG 2 0.0915 Tier III
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Other clones with same target sequence:

(none)