Construct: sgRNA BRDN0001146485
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGCGGAAGCTACTTCGTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PI4K2A (55361)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76820
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
97641161 |
+ |
PI4K2A |
NNNCGGAAGCTACTTCGTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
236350953 |
+ |
IQCA1 |
NNNCGGAAGCTACTTCGTCC |
NGG |
1 |
0.2273 |
Tier II |
| 3 |
NC_000002.12 |
2 |
236350953 |
+ |
LOC105373945 |
NNNCGGAAGCTACTTCGTCC |
NGG |
1 |
0.2273 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
42090920 |
+ |
Pi4k2a |
NNNTGGAAGCTACTTCGTCA |
NGG |
1 |
0.8 |
Tier I |
| 2 |
NC_000078.6 |
12 |
101711312 |
+ |
Tc2n |
NNNAGGAAGCTACTTCGCCA |
NGG |
2 |
0.3609 |
Tier II |
| 3 |
NC_000071.6 |
5 |
102912415 |
+ |
Mapk10 |
NNNCGGAAGCTACATCTTCA |
NGG |
2 |
0.1548 |
Tier II |
| 4 |
NC_000075.6 |
9 |
62999134 |
+ |
Gm35574 |
NNNCGGAAGCTGCTTCCTCA |
NGG |
2 |
0.1699 |
Tier III |
| 5 |
NC_000080.6 |
14 |
20892923 |
- |
Gm30212 |
NNNCGGAAGCTACTTCCTAA |
NGG |
2 |
0.1008 |
Tier III |
Other clones with same target sequence:
(none)
