Construct: sgRNA BRDN0001146489
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTCCGAATTCGCCCTTGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRMS (6725)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76648
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
63542531 |
- |
SRMS |
NNNCCGAATTCGCCCTTGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
43481944 |
- |
ADAMTS20 |
NNNCCCAATTCACCCTTGGG |
NGG |
2 |
0.6364 |
Tier II |
| 3 |
NC_000004.12 |
4 |
87498725 |
- |
SPARCL1 |
NNNCCTAATTCACCCTTGGG |
NGG |
2 |
0.6222 |
Tier II |
| 4 |
NC_000008.11 |
8 |
142732384 |
- |
THEM6 |
NNNCCTAATTCTCCCTTGGG |
NGG |
2 |
0.2564 |
Tier II |
| 5 |
NC_000019.10 |
19 |
12440825 |
+ |
ZNF443 |
NNNCCGAAGTCGCCCTTGGG |
NAG |
2 |
0.1605 |
Tier II |
| 6 |
NC_000003.12 |
3 |
172647258 |
+ |
NCEH1 |
NNNCCGAATTCCGCCTTGGG |
NGG |
2 |
0.0722 |
Tier II |
| 7 |
NC_000015.10 |
15 |
75032736 |
- |
PPCDC |
NNNCCGAATTTGGCCTTGGG |
NGG |
2 |
0.042 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
151268248 |
- |
Stard13 |
NNNCAGAATTCACCCTTGGG |
NGG |
2 |
0.5333 |
Tier II |
Other clones with same target sequence:
(none)
