Construct: sgRNA BRDN0001146490
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGAATACATACTACACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROR2 (4920)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77940
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
91730914 |
+ |
ROR2 |
NNNGAATACATACTACACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
99112247 |
- |
TSGA10 |
NNNGAATATATACTACATGA |
NGG |
2 |
0.5625 |
Tier II |
| 3 |
NC_000023.11 |
X |
84063056 |
+ |
RPS6KA6 |
NNNGAATACATACTACACAG |
NGG |
2 |
0.5462 |
Tier II |
| 4 |
NC_000003.12 |
3 |
52978811 |
+ |
SFMBT1 |
NNNGAATACATACTACAACA |
NGG |
2 |
0.2414 |
Tier II |
| 5 |
NC_000004.12 |
4 |
84833141 |
+ |
WDFY3 |
NNNGAATACATACTACACAA |
NAG |
2 |
0.1852 |
Tier II |
| 6 |
NC_000007.14 |
7 |
75615104 |
+ |
HIP1 |
NNNGAATACCTACTACACGC |
NGG |
2 |
0.0758 |
Tier II |
| 7 |
NC_000018.10 |
18 |
44402130 |
- |
LINC01478 |
NNNGAATACATGCTACATGA |
NGG |
2 |
0.4643 |
Tier III |
| 8 |
NC_000001.11 |
1 |
221896353 |
+ |
LOC124904517 |
NNNGAAGACATACTACACCA |
NGG |
2 |
0.2637 |
Tier III |
| 9 |
NC_000019.10 |
19 |
27994437 |
+ |
LINC02987 |
NNNGAATACATACTAGACGA |
NGG |
1 |
0.1538 |
Tier III |
| 10 |
NC_000019.10 |
19 |
27994437 |
+ |
LOC105372346 |
NNNGAATACATACTAGACGA |
NGG |
1 |
0.1538 |
Tier III |
| 11 |
NC_000005.10 |
5 |
172488084 |
+ |
LOC105377727 |
NNNGAATACATTCTCCACGA |
NGG |
2 |
0.0909 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
53117139 |
+ |
Ror2 |
NNNGAATACATACTACACGG |
NGG |
1 |
0.7647 |
Tier I |
| 2 |
NC_000070.6 |
4 |
148150770 |
+ |
Fbxo6 |
NNNGAATATTTACTACACGA |
NGG |
2 |
0.7721 |
Tier II |
| 3 |
NC_000086.7 |
X |
129821116 |
+ |
Diaph2 |
NNNGAATACATACTAAATGA |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000083.6 |
17 |
65503288 |
+ |
Tmem232 |
NNNGAATACACACTACACAA |
NGG |
2 |
0.4762 |
Tier II |
| 5 |
NC_000073.6 |
7 |
12838746 |
- |
Zfp110 |
NNNGAATACATACTACACCA |
NGG |
1 |
0.4483 |
Tier II |
| 6 |
NC_000086.7 |
X |
130334351 |
+ |
Diaph2 |
NNNGAATACATACCACAAGA |
NGG |
2 |
0.1538 |
Tier II |
| 7 |
NC_000082.6 |
16 |
37159189 |
+ |
Stxbp5l |
NNNGAATAAATACTACAGGA |
NGG |
2 |
0.1143 |
Tier II |
| 8 |
NC_000069.6 |
3 |
126773872 |
+ |
Camk2d |
NNNGAATACATTCTACACGA |
NAG |
2 |
0.0864 |
Tier II |
| 9 |
NC_000068.7 |
2 |
52297465 |
+ |
Neb |
NNNGAATACATACGACAAGA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000068.7 |
2 |
52297465 |
+ |
4930573O16Rik |
NNNGAATACATACGACAAGA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
