Construct: sgRNA BRDN0001146491
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTGGGTCATGCCCACAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- COQ8A (56997)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77323
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
226965157 |
+ |
COQ8A |
NNNGGGTCATGCCCACAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
73704747 |
+ |
STX1A |
NNNGGACCATGCCCACAGCG |
NGG |
2 |
0.6875 |
Tier II |
| 3 |
NC_000017.11 |
17 |
51713333 |
+ |
CA10 |
NNNGGGTCATGACCACAGCA |
NGG |
2 |
0.6696 |
Tier II |
| 4 |
NC_000012.12 |
12 |
123059031 |
+ |
PITPNM2 |
NNNGGGCCAAGCCCACAGCG |
NGG |
2 |
0.5893 |
Tier II |
| 5 |
NC_000023.11 |
X |
119151038 |
- |
KIAA1210 |
NNNAGGGCATGCCCACAGCG |
NGG |
2 |
0.5294 |
Tier II |
| 6 |
NC_000007.14 |
7 |
73704747 |
+ |
LOC105375350 |
NNNGGACCATGCCCACAGCG |
NGG |
2 |
0.6875 |
Tier III |
| 7 |
NC_000020.11 |
20 |
62565207 |
+ |
MIR1-1HG |
NNNGGGTCATCCCCACAGCT |
NGG |
2 |
0.3 |
Tier III |
| 8 |
NC_000015.10 |
15 |
88308736 |
+ |
LOC105370958 |
NNNGGGTCTTTCCCACAGCG |
NGG |
2 |
0.2308 |
Tier III |
| 9 |
NC_000002.12 |
2 |
113694957 |
- |
LINC02936 |
NNNGGGGCATTCCCACAGCG |
NGG |
2 |
0.2262 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
152096894 |
+ |
Plekhg5 |
NNNGGGTCATGCCCACAGCA |
NCG |
2 |
0.1004 |
Tier I |
| 2 |
NC_000085.6 |
19 |
23901949 |
- |
Apba1 |
NNNGGATCCTGCCCACAGCG |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000068.7 |
2 |
5402928 |
+ |
Camk1d |
NNNGGGTCATGCCCATAGCA |
NGG |
2 |
0.2885 |
Tier II |
| 4 |
NC_000084.6 |
18 |
33972194 |
- |
Epb41l4a |
NNNGGCTCATGCCCTCAGCG |
NGG |
2 |
0.1364 |
Tier II |
| 5 |
NC_000084.6 |
18 |
35680741 |
+ |
Dnajc18 |
NNNGGGTCATGGCCACAGGG |
NGG |
2 |
0.0556 |
Tier II |
Other clones with same target sequence:
(none)
