Construct: sgRNA BRDN0001146492
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCTACGGGCTTGTGCACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCG (5582)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76899
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
53889730 |
+ |
PRKCG |
NNNTACGGGCTTGTGCACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
12806063 |
- |
CAND2 |
NNNTACAGGCATGTGCACCA |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000014.9 |
14 |
90000363 |
+ |
TDP1 |
NNNTACAGGCATGTGCACCA |
NGG |
2 |
0.75 |
Tier II |
| 4 |
NC_000014.9 |
14 |
93646883 |
+ |
UNC79 |
NNNTACCAGCTTGTGCACCA |
NGG |
2 |
0.6875 |
Tier II |
| 5 |
NC_000011.10 |
11 |
103950982 |
- |
PDGFD |
NNNTAGGGGCTTGTGCACCA |
NAG |
2 |
0.1296 |
Tier II |
| 6 |
NC_000009.12 |
9 |
125756013 |
+ |
PBX3 |
NNNTATGGGCTTGTGCACCA |
NTG |
2 |
0.0362 |
Tier II |
| 7 |
NC_000002.12 |
2 |
24948087 |
+ |
DNAJC27 |
NNNTAAGGGCTTGGGCACCA |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
3310656 |
+ |
Prkcg |
NNNTACGGGCTGGTGCACCA |
NGG |
1 |
0.5 |
Tier I |
| 2 |
NC_000081.6 |
15 |
27829404 |
+ |
Trio |
NNNTATGGCCTTGTGCACCA |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000076.6 |
10 |
76105080 |
- |
Slc5a4b |
NNNTACTGGCTTGTTCACCA |
NGG |
2 |
0.0816 |
Tier II |
| 4 |
NC_000086.7 |
X |
51660076 |
+ |
Hs6st2 |
NNNTACTGGCTTGTTCACCA |
NGG |
2 |
0.0816 |
Tier II |
| 5 |
NC_000072.6 |
6 |
53665424 |
- |
Creb5 |
NNNTTCGGGCTTGTGCCCCA |
NGG |
2 |
0.0642 |
Tier II |
| 6 |
NC_000078.6 |
12 |
50637620 |
+ |
Prkd1 |
NNNTACAGGCTTGTGCACCA |
NTG |
2 |
0.039 |
Tier II |
| 7 |
NC_000074.6 |
8 |
114067505 |
+ |
Gm30107 |
NNNTACAGGCTTGAGCACCA |
NGG |
2 |
0.619 |
Tier III |
| 8 |
NC_000071.6 |
5 |
125945579 |
- |
1700048F04Rik |
NNNTACTGGCTTGTACACCA |
NGG |
2 |
0.5378 |
Tier III |
Other clones with same target sequence:
(none)
