Construct: sgRNA BRDN0001146493
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAAGATCGGAACCGGTCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCLK1 (9201)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76748
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
36125903 |
+ |
DCLK1 |
NNNAGATCGGAACCGGTCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
139299464 |
- |
UBN2 |
NNNAGATCTGAACCGATCTG |
NGG |
2 |
0.5333 |
Tier II |
| 3 |
NC_000015.10 |
15 |
58468722 |
- |
LIPC |
NNNAGATCTGAACTGGTCTG |
NGG |
2 |
0.3911 |
Tier II |
| 4 |
NC_000004.12 |
4 |
48780203 |
+ |
FRYL |
NNNCGATCGGAACCGATCTG |
NGG |
2 |
0.3529 |
Tier II |
| 5 |
NC_000015.10 |
15 |
61195456 |
- |
RORA |
NNNAGATCGGAACCCCTCTG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000015.10 |
15 |
58468722 |
- |
LIPC-AS1 |
NNNAGATCTGAACTGGTCTG |
NGG |
2 |
0.3911 |
Tier III |
| 7 |
NC_000012.12 |
12 |
15829528 |
- |
LOC102724146 |
NNNAGATTGGTACCGGTCTG |
NGG |
2 |
0.2692 |
Tier III |
| 8 |
NC_000001.11 |
1 |
199337974 |
+ |
LINC02789 |
NNNAGATCGGAACTGGGCTG |
NGG |
2 |
0.0863 |
Tier III |
| 9 |
NC_000015.10 |
15 |
61195456 |
- |
LOC105370841 |
NNNAGATCGGAACCCCTCTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
55247386 |
- |
Dclk1 |
NNNAGATCTGAAGCGGTCTG |
NGG |
2 |
0.0727 |
Tier I |
| 2 |
NC_000083.6 |
17 |
62650028 |
- |
Efna5 |
NNNAGATGTGAACCGGTCTG |
NGG |
2 |
0.3429 |
Tier II |
| 3 |
NC_000068.7 |
2 |
36055817 |
+ |
Morn5 |
NNNAGATGGCAACCGGTCTG |
NGG |
2 |
0.2571 |
Tier II |
Other clones with same target sequence:
(none)
