Construct: sgRNA BRDN0001146496
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGGATGATGCTACTAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DSTYK (25778)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76298
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
205169178 |
- |
DSTYK |
NNNGGATGATGCTACTAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
108728722 |
+ |
FNDC7 |
NNNTGATGATGCTACTTACA |
NGG |
2 |
0.0485 |
Tier I |
| 3 |
NC_000003.12 |
3 |
46441041 |
- |
LTF |
NNNGGATGATGCTACAAACT |
NGG |
2 |
0.5455 |
Tier II |
| 4 |
NC_000002.12 |
2 |
227525373 |
- |
AGFG1 |
NNNGAATGATGCTACTAATA |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000009.12 |
9 |
98533961 |
+ |
GABBR2 |
NNNGGATGATGATTCTAACA |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000002.12 |
2 |
206613728 |
- |
ADAM23 |
NNNGGAAGATGCTACTAAAA |
NGG |
2 |
0.375 |
Tier II |
| 7 |
NC_000003.12 |
3 |
119916765 |
- |
GSK3B |
NNNTGGTGATGCTACTAACA |
NGG |
2 |
0.2597 |
Tier II |
| 8 |
NC_000016.10 |
16 |
81338918 |
+ |
GAN |
NNNGCATGATGCTACTAAGA |
NGG |
2 |
0.0982 |
Tier II |
| 9 |
NC_000014.9 |
14 |
74875893 |
+ |
PROX2 |
NNNGGATGATGTTACTCACA |
NGG |
2 |
0.095 |
Tier II |
| 10 |
NC_000016.10 |
16 |
17148195 |
+ |
XYLT1 |
NNNGGATGATGCTTCTCACA |
NGG |
2 |
0.0941 |
Tier II |
| 11 |
NC_000001.11 |
1 |
230162364 |
+ |
GALNT2 |
NNNGGGTGATGCTACTAAGA |
NGG |
2 |
0.0893 |
Tier II |
| 12 |
NC_000001.11 |
1 |
40271638 |
- |
ZMPSTE24 |
NNNGGATAATGCTACTAACA |
NTG |
2 |
0.039 |
Tier II |
| 13 |
NC_000003.12 |
3 |
54578689 |
+ |
CACNA2D3 |
NNNGGATGATGCCACTAACA |
NGC |
2 |
0.0175 |
Tier II |
| 14 |
NC_000007.14 |
7 |
64864152 |
+ |
EEF1DP4 |
NNNGGATGATGAGACTAACA |
NGG |
2 |
0.1863 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
132449961 |
+ |
Dstyk |
NNNGGATGATGCTGCCAACA |
NGG |
2 |
0.3111 |
Tier I |
| 2 |
NC_000070.6 |
4 |
110772793 |
+ |
Agbl4 |
NNNGAAAGATGCTACTAACA |
NGG |
2 |
0.7583 |
Tier II |
| 3 |
NC_000085.6 |
19 |
41513663 |
+ |
Lcor |
NNNGAATGATGCTTCTAACA |
NGG |
2 |
0.4622 |
Tier II |
| 4 |
NC_000075.6 |
9 |
29691561 |
- |
Ntm |
NNNGGATGATTCTACCAACA |
NGG |
2 |
0.2564 |
Tier II |
| 5 |
NC_000083.6 |
17 |
12156329 |
- |
Agpat4 |
NNNGGATGATGCTCCAAACA |
NGG |
2 |
0.1948 |
Tier II |
| 6 |
NC_000078.6 |
12 |
72089704 |
+ |
Jkamp |
NNNGTATGATGTTACTAACA |
NGG |
2 |
0.1615 |
Tier II |
| 7 |
NC_000077.6 |
11 |
110202625 |
- |
Abca6 |
NNNTTATGATGCTACTAACA |
NGG |
2 |
0.1091 |
Tier II |
| 8 |
NC_000071.6 |
5 |
89545206 |
- |
Npffr2 |
NNNGGATGATGCTAAGAACA |
NGG |
2 |
0.0769 |
Tier II |
| 9 |
NC_000079.6 |
13 |
58587034 |
+ |
Slc28a3 |
NNNGGATGATGCTACTGGCA |
NGG |
2 |
0.0706 |
Tier II |
| 10 |
NC_000078.6 |
12 |
82178703 |
- |
Sipa1l1 |
NNNGGATGATACTACTAACA |
NGA |
2 |
0.0694 |
Tier II |
| 11 |
NC_000083.6 |
17 |
10715806 |
+ |
Pacrg |
NNNGGATGATTCTACTCACA |
NGG |
2 |
0.0679 |
Tier II |
| 12 |
NC_000079.6 |
13 |
112771744 |
- |
Gm41067 |
NNNGGATGATGCTACCAACG |
NGG |
2 |
0.5098 |
Tier III |
| 13 |
NC_000070.6 |
4 |
127914301 |
+ |
Gm40256 |
NNNGGATGATGCTACTAACG |
NGA |
2 |
0.0531 |
Tier III |
| 14 |
NC_000085.6 |
19 |
32754831 |
+ |
Gm36566 |
NNNGGGTGATGCTAGTAACA |
NGG |
2 |
0.0357 |
Tier III |
Other clones with same target sequence:
(none)
