Construct: sgRNA BRDN0001146503

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGCTTGGTGTGCGATCCAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAGK (55577)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76324

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 71073488 - NAGK NNNTTGGTGTGCGATCCAGT NGG 0 1.0 Tier I
2 NC_000007.14 7 100631241 + TFR2 NNNTAGGTGTGCAATCCAGT NGG 2 0.4615 Tier II
3 NC_000016.10 16 2895336 + FLYWCH2 NNNTTGGTGTGAGAACCAGT NGG 2 0.4135 Tier II
4 NC_000023.11 X 106863233 + TBC1D8B NNNTTGGGCTGCGATCCAGT NGG 2 0.3949 Tier II
5 NC_000001.11 1 171928147 + DNM3 NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier II
6 NC_000002.12 2 205519886 + PARD3B NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier II
7 NC_000003.12 3 180634776 - CCDC39 NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier II
8 NC_000004.12 4 90880031 + CCSER1 NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier II
9 NC_000010.11 10 125977395 + FANK1 NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier II
10 NC_000001.11 1 69880463 + LRRC7 NNNTTGGTGTGCTATGCAGT NGG 2 0.0462 Tier II
11 NC_000023.11 X 113345775 + LOC101928437 NNNTTGAGGTGCGATCCAGT NGG 2 0.7333 Tier III
12 NC_000007.14 7 100631241 + LOC124901709 NNNTAGGTGTGCAATCCAGT NGG 2 0.4615 Tier III
13 NC_000003.12 3 14034996 + TPRXL NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier III
14 NC_000004.12 4 19294848 + LINC02438 NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier III
15 NC_000006.12 6 133864747 + TARID NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier III
16 NC_000010.11 10 79748839 - NUTM2B-AS1 NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier III
17 NC_000010.11 10 87271051 - NUTM2A-AS1 NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier III
18 NC_000010.11 10 87466923 + LOC112268063 NNNTTGGGGTGTGATCCAGT NGG 2 0.3949 Tier III
19 NC_000005.10 5 21061375 + LOC105374678 NNNCTGGTGTGTGATCCAGT NGG 2 0.3484 Tier III
20 NC_000019.10 19 35953053 - LOC105372383 NNNTGGGTGTGGGATCCAGT NGG 2 0.2844 Tier III
21 NC_000023.11 X 115901009 - DANT2 NNNTTGGTGTGGGATCCTGT NGG 2 0.2222 Tier III
22 NC_000023.11 X 115901009 - DANT1 NNNTTGGTGTGGGATCCTGT NGG 2 0.2222 Tier III
23 NC_000019.10 19 19827251 + ZNF56P NNNTTGGTGTGCGAGCCAGG NGG 2 0.0088 Tier III
24 NC_000019.10 19 19827251 + ISCA1P5 NNNTTGGTGTGCGAGCCAGG NGG 2 0.0088 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 83799277 - Nagk NNNTTGGTGTGCAATCCAGT NGG 1 0.9231 Tier I
2 NC_000080.6 14 14011501 + Atxn7 NNNTTGGTGTGCAATCCAGT NGG 1 0.9231 Tier II
3 NC_000071.6 5 89875314 - Adamts3 NNNTTGGTATGCGATCTAGT NGG 2 0.3 Tier II
4 NC_000073.6 7 118950405 - Iqck NNNTTGGTGTGCCATCCTGT NGG 2 0.2105 Tier II
5 NC_000082.6 16 95407069 - Erg NNNTTGGTGTGGGAGCCAGT NGG 2 0.0222 Tier II
6 NC_000079.6 13 10564569 + Gm26861 NNNTTGGTGTGCGGTTCAGT NGG 2 0.1436 Tier III
7 NC_000083.6 17 36138908 + Gm9574 NNNTTGGTGTGCGGGCCAGT NGG 2 0.0233 Tier III
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Other clones with same target sequence:

(none)