Construct: sgRNA BRDN0001146504

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATGATGGCAAACAACCCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTK (7272)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75580

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 80007854 + TTK NNNGATGGCAAACAACCCAG NGG 0 1.0 Tier I
2 NC_000010.11 10 59840367 - CCDC6 NNNGAAAGCAAACAACCCAG NGG 2 0.8667 Tier II
3 NC_000016.10 16 3023128 + HCFC1R1 NNNGATGGGAAACAACCCAG NGG 1 0.619 Tier II
4 NC_000020.11 20 38868100 - PPP1R16B NNNGATGGAAAACAGCCCAG NGG 2 0.5571 Tier II
5 NC_000017.11 17 12056784 - MAP2K4 NNNGATGGCAAACAACTCAG NGG 1 0.4667 Tier II
6 NC_000007.14 7 108052791 - LAMB4 NNNGATGGAAAAAAACCCAG NGG 2 0.3297 Tier II
7 NC_000011.10 11 71100364 + SHANK2 NNNAATGGCAAACAATCCAG NGG 2 0.2769 Tier II
8 NC_000010.11 10 70410504 - EIF4EBP2 NNNTATGGCAAATAACCCAG NGG 2 0.2545 Tier II
9 NC_000022.11 22 41125570 - EP300 NNNGATGGCAAAAAACCAAG NGG 2 0.2071 Tier II
10 NC_000002.12 2 9384749 - ASAP2 NNNGATGGCAAACCACACAG NGG 2 0.1 Tier II
11 NC_000001.11 1 40071548 - PPT1 NNNGAGGGCAAAGAACCCAG NGG 2 0.0779 Tier II
12 NC_000001.11 1 40071548 - CAP1 NNNGAGGGCAAAGAACCCAG NGG 2 0.0779 Tier II
13 NC_000004.12 4 98044399 - STPG2 NNNGAGGGCAAAGAACCCAG NGG 2 0.0779 Tier II
14 NC_000012.12 12 98974633 + ANKS1B NNNGATGGCAAACAACCCAT NGA 2 0.0486 Tier II
15 NC_000003.12 3 8673595 - SSUH2 NNNGATGGCAATCAACCCAG NTG 2 0.013 Tier II
16 NC_000005.10 5 173656574 + LINC01863 NNNAATGGCAAACAACTCAG NGG 2 0.42 Tier III
17 NC_000010.11 10 95125121 - LOC107984257 NNNGATGGGAAACTACCCAG NGG 2 0.3302 Tier III
18 NC_000002.12 2 9384749 - LOC124907730 NNNGATGGCAAACCACACAG NGG 2 0.1 Tier III
19 NC_000010.11 10 43604829 + CAP1P2 NNNGAGGGCAAAGAACCCAG NGG 2 0.0779 Tier III
20 NC_000002.12 2 237134143 - LOC124906127 NNNGATGGCAAACAACCCAT NCG 2 0.075 Tier III
21 NC_000016.10 16 13802673 + LOC124903646 NNNAATGGCAAACAACCCAG NTG 2 0.0351 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 141474867 - Spen NNNGCTGGCAAACAACCCAG NCG 2 0.0536 Tier I
2 NC_000067.6 1 51779399 - Myo1b NNNGATGGAAAACAAACCAG NGG 2 0.8571 Tier II
3 NC_000068.7 2 7171793 + Celf2 NNNGCTGGCAAACAAACCAG NGG 2 0.5 Tier II
4 NC_000068.7 2 35316882 + Stom NNNGATGGCAAACAAAACAG NGG 2 0.4667 Tier II
5 NC_000068.7 2 124595222 + Sema6d NNNTATGGCAAACAAACCAG NGG 2 0.3636 Tier II
6 NC_000074.6 8 124315746 - Galnt2 NNNGATGGCTCACAACCCAG NGG 2 0.3529 Tier II
7 NC_000071.6 5 66939306 - Limch1 NNNTATGGAAAACAACCCAG NGG 2 0.3117 Tier II
8 NC_000079.6 13 108335817 + Depdc1b NNNGATGGCACACAGCCCAG NGG 2 0.26 Tier II
9 NC_000078.6 12 84685076 + Syndig1l NNNGATGGCATACAACCCAT NGG 2 0.2154 Tier II
10 NC_000077.6 11 34462193 + Dock2 NNNCATGGCCAACAACCCAG NGG 2 0.1765 Tier II
11 NC_000080.6 14 121962208 - Ubac2 NNNGATGGCCAACGACCCAG NGG 2 0.1556 Tier II
12 NC_000080.6 14 121962208 - Gpr183 NNNGATGGCCAACGACCCAG NGG 2 0.1556 Tier II
13 NC_000071.6 5 92463705 + Scarb2 NNNGATGACAAACAAGCCAG NGG 2 0.1538 Tier II
14 NC_000067.6 1 170431795 - Nos1ap NNNGACGGCAAACAAGCCAG NGG 2 0.1399 Tier II
15 NC_000077.6 11 93922815 - Mbtd1 NNNGATGGCAAACACCACAG NGG 2 0.1273 Tier II
16 NC_000078.6 12 66668074 - Mdga2 NNNGATGGCAAACAAGCCAT NGG 2 0.1077 Tier II
17 NC_000084.6 18 67994030 - Ldlrad4 NNNGAGGGCAAACAAGCCAG NGG 2 0.0879 Tier II
18 NC_000072.6 6 32316769 + Plxna4 NNNGATGGGAAAGAACCCAG NGG 2 0.0844 Tier II
19 NC_000073.6 7 38223465 + Plekhf1 NNNGATGGCAAACTAGCCAG NGG 2 0.0821 Tier II
20 NC_000080.6 14 23738922 + Kcnma1 NNNGATGGCAAACAATCCAG NAG 2 0.0798 Tier II
21 NC_000071.6 5 30521550 - Cib4 NNNGATGGCAAAGTACCCAG NGG 2 0.0727 Tier II
22 NC_000075.6 9 110584001 - Setd2 NNNGATGGCAAACAAGACAG NGG 2 0.0718 Tier II
23 NC_000080.6 14 101686151 - Uchl3 NNNGATGGCAAAGAACTCAG NGG 2 0.0636 Tier II
24 NC_000077.6 11 65220409 - Myocd NNNGATGGCACAGAACCCAG NGG 2 0.0545 Tier II
25 NC_000075.6 9 55230978 - Nrg4 NNNGATGGAAAACAACCCAG NTG 2 0.0334 Tier II
26 NC_000085.6 19 42180623 - Zfyve27 NNNGATGGCAAACAACACAG NGA 2 0.0324 Tier II
27 NC_000070.6 4 155388540 + Gabrd NNNGATGGCACACAACCCAG NGA 2 0.0278 Tier II
28 NC_000076.6 10 19504636 - Gm33104 NNNGATAGCAATCAACCCAG NGG 2 0.3333 Tier III
29 NC_000073.6 7 136078988 + Gm51502 NNNGATGGAAAACAACCCAG NCG 2 0.0918 Tier III
30 NC_000073.6 7 48764426 + Gm32263 NNNCATGGCAAACAACCCAG NTG 2 0.0206 Tier III
31 NC_000077.6 11 66189384 + Gm12296 NNNGATGGCAAACAACCCTG NGT 2 0.0087 Tier III
Download CSV

Other clones with same target sequence:

(none)