Construct: sgRNA BRDN0001146505
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GACTAAGACTAGCTCCAGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL5 (6792)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77195
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
18604040 |
- |
CDKL5 |
NNNTAAGACTAGCTCCAGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
128904176 |
- |
KCNJ5 |
NNNTAATAATAGCTCCAGCA |
NGG |
2 |
0.4898 |
Tier II |
| 3 |
NC_000011.10 |
11 |
33389932 |
+ |
KIAA1549L |
NNNTAAAACTAGCTCCAGTA |
NGG |
2 |
0.4615 |
Tier II |
| 4 |
NC_000012.12 |
12 |
119008464 |
- |
SRRM4 |
NNNTAAGAGTAGCTCCAACA |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000012.12 |
12 |
119008506 |
- |
SRRM4 |
NNNTAAGAGTAGCTCCAACA |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000016.10 |
16 |
49626142 |
+ |
ZNF423 |
NNNAAAGAGTAGCTCCAGCA |
NGG |
2 |
0.2948 |
Tier II |
| 7 |
NC_000011.10 |
11 |
132455550 |
- |
OPCML |
NNNAAAGACTATCTCCAGCA |
NGG |
2 |
0.1832 |
Tier II |
| 8 |
NC_000007.14 |
7 |
91157739 |
+ |
CDK14 |
NNNTTAGACTAGCTCCTGCA |
NGG |
2 |
0.0485 |
Tier II |
| 9 |
NC_000007.14 |
7 |
21608442 |
- |
DNAH11 |
NNNTAAGACTATCTCCAGCA |
NGA |
2 |
0.0267 |
Tier II |
| 10 |
NC_000015.10 |
15 |
76902045 |
+ |
SCAPER |
NNNTAAGACTAGCTCCATCA |
NTG |
2 |
0.026 |
Tier II |
| 11 |
NC_000011.10 |
11 |
128904176 |
- |
KCNJ5-AS1 |
NNNTAATAATAGCTCCAGCA |
NGG |
2 |
0.4898 |
Tier III |
| 12 |
NC_000005.10 |
5 |
130868043 |
- |
LOC107986449 |
NNNTAAAACTAGCTGCAGCA |
NGG |
2 |
0.05 |
Tier III |
| 13 |
NC_000007.14 |
7 |
25638367 |
+ |
LINC03007 |
NNNTAGGACTAGCTCCAGCA |
NGA |
2 |
0.0496 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
69404785 |
- |
Tcf4 |
NNNTAAGGCTAGCTCCAGCT |
NGG |
2 |
0.44 |
Tier II |
| 2 |
NC_000086.7 |
X |
151471357 |
- |
Fam120c |
NNNTAAGATTATCTCCAGCA |
NGG |
2 |
0.3365 |
Tier II |
| 3 |
NC_000080.6 |
14 |
63993488 |
+ |
Rp1l1 |
NNNTCAGACCAGCTCCAGCA |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000074.6 |
8 |
123125059 |
+ |
Cpne7 |
NNNTCAGACTACCTCCAGCA |
NGG |
2 |
0.2647 |
Tier II |
| 5 |
NC_000075.6 |
9 |
31859237 |
+ |
Barx2 |
NNNTAAGCCTAGCTCCAGCT |
NGG |
2 |
0.2571 |
Tier II |
| 6 |
NC_000077.6 |
11 |
33802392 |
+ |
Kcnip1 |
NNNTAAGACTGGATCCAGCA |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000077.6 |
11 |
103249378 |
- |
Map3k14 |
NNNCTAGACTAGCTCCAGCA |
NGG |
2 |
0.2353 |
Tier II |
| 8 |
NC_000074.6 |
8 |
69971216 |
- |
Gatad2a |
NNNTAAGACTGGCTCCAGCA |
NAG |
2 |
0.1685 |
Tier II |
| 9 |
NC_000075.6 |
9 |
89953317 |
+ |
Rasgrf1 |
NNNTAAGGCTAGCTCCAGCC |
NGG |
2 |
0.1667 |
Tier II |
| 10 |
NC_000068.7 |
2 |
165858839 |
+ |
Zmynd8 |
NNNTAAGACTTCCTCCAGCA |
NGG |
2 |
0.1629 |
Tier II |
| 11 |
NC_000072.6 |
6 |
67289634 |
+ |
Il12rb2 |
NNNTAAGTCTAGCTCTAGCA |
NGG |
2 |
0.1319 |
Tier II |
| 12 |
NC_000070.6 |
4 |
148268954 |
- |
Disp3 |
NNNTAAGCCTAGCCCCAGCA |
NGG |
2 |
0.1224 |
Tier II |
| 13 |
NC_000077.6 |
11 |
55266524 |
- |
Fat2 |
NNNTAAGACAAGCTCCAGGA |
NGG |
2 |
0.1071 |
Tier II |
| 14 |
NC_000070.6 |
4 |
133865574 |
+ |
Rps6ka1 |
NNNTAAGTCTAGCTGCAGCA |
NGG |
2 |
0.0214 |
Tier II |
| 15 |
NC_000080.6 |
14 |
124302189 |
- |
Fgf14 |
NNNTAAGACTAGATCCAGCA |
NTG |
2 |
0.015 |
Tier II |
| 16 |
NC_000080.6 |
14 |
48506312 |
+ |
Tmem260 |
NNNTAAGTCTAGCTCCAGCA |
NGC |
2 |
0.0095 |
Tier II |
| 17 |
NC_000086.7 |
X |
137777813 |
- |
Il1rapl2 |
NNNTAAGACTAGCTCCAGTA |
NGT |
2 |
0.0074 |
Tier II |
| 18 |
NC_000076.6 |
10 |
26698918 |
- |
Gm48893 |
NNNTCAGACTATCTCCAGCA |
NGG |
2 |
0.1923 |
Tier III |
| 19 |
NC_000069.6 |
3 |
74125864 |
- |
Gm36069 |
NNNTAAGACTAACTCCAGCA |
NGT |
2 |
0.0151 |
Tier III |
Other clones with same target sequence:
(none)
