Construct: sgRNA BRDN0001146506
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGCCGCGTCAACCTCATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GALK1 (2584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76703
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
75765016 |
- |
GALK1 |
NNNCCGCGTCAACCTCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
129719402 |
+ |
PRRX2 |
NNNGCGCGTCAACCTCAGCG |
NGG |
2 |
0.1667 |
Tier I |
| 3 |
NC_000007.14 |
7 |
100091301 |
+ |
COPS6 |
NNNCCGCGTCAAGCTCATCT |
NGG |
2 |
0.0955 |
Tier I |
| 4 |
NC_000019.10 |
19 |
2078338 |
- |
MOB3A |
NNNCCGCGTCAACCTCATCT |
NCG |
2 |
0.075 |
Tier I |
| 5 |
NC_000017.11 |
17 |
33514405 |
+ |
ASIC2 |
NNNCCCCCTCAACCTCATCG |
NGG |
2 |
0.4196 |
Tier II |
| 6 |
NC_000005.10 |
5 |
180027945 |
+ |
RNF130 |
NNNCCGCATCAACCTCATCG |
NGA |
2 |
0.0694 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
116012558 |
- |
Galk1 |
NNNCCGCGTCAACCTCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000083.6 |
17 |
80556398 |
- |
Cdkl4 |
NNNCCTCGTGAACCTCATCG |
NGG |
2 |
0.2593 |
Tier I |
| 3 |
NC_000069.6 |
3 |
27709849 |
+ |
Fndc3b |
NNNCAGCGTCAGCCTCATCG |
NGG |
2 |
0.4127 |
Tier II |
Other clones with same target sequence:
(none)
