Construct: sgRNA BRDN0001146507

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAGAATCCATCTACCGTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKCI (5584)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76400

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 170267928 + PRKCI NNNGAATCCATCTACCGTAG NGG 0 1.0 Tier I
2 NC_000006.12 6 150397740 - IYD NNNGAATCCAACTACAGTAG NGG 2 0.75 Tier II
3 NC_000007.14 7 126663177 - GRM8 NNNGAATCCATCTGCCGTAG NGC 2 0.0104 Tier II
4 NC_000023.11 X 101538903 - PRKCIP1 NNNAAATCCATCTACCGTAG NGG 1 0.9 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 85269651 - Camkmt NNNGAATCCATATACCATAG NGG 2 0.6667 Tier II
2 NC_000078.6 12 61609110 - Lrfn5 NNNGAATCCATCTACTGAAG NGG 2 0.2051 Tier II
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Other clones with same target sequence:

(none)