Construct: sgRNA BRDN0001146507
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGAATCCATCTACCGTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCI (5584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76400
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000003.12 |
3 |
170267928 |
+ |
PRKCI |
NNNGAATCCATCTACCGTAG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000006.12 |
6 |
150397740 |
- |
IYD |
NNNGAATCCAACTACAGTAG |
NGG |
2 |
0.75 |
Tier II |
3 |
NC_000007.14 |
7 |
126663177 |
- |
GRM8 |
NNNGAATCCATCTGCCGTAG |
NGC |
2 |
0.0104 |
Tier II |
4 |
NC_000023.11 |
X |
101538903 |
- |
PRKCIP1 |
NNNAAATCCATCTACCGTAG |
NGG |
1 |
0.9 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000083.6 |
17 |
85269651 |
- |
Camkmt |
NNNGAATCCATATACCATAG |
NGG |
2 |
0.6667 |
Tier II |
2 |
NC_000078.6 |
12 |
61609110 |
- |
Lrfn5 |
NNNGAATCCATCTACTGAAG |
NGG |
2 |
0.2051 |
Tier II |
Other clones with same target sequence:
(none)