Construct: sgRNA BRDN0001146514
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTCTCCACAGATCCACCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IDNK (414328)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75865
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
83628187 |
+ |
IDNK |
NNNCTCCACAGATCCACCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
57475952 |
- |
DOK4 |
NNNCTCCACAGATCTACCGG |
NGG |
2 |
0.0118 |
Tier I |
| 3 |
NC_000018.10 |
18 |
76377571 |
- |
ZNF516 |
NNNCTCCCCAGATCCACCGT |
NGG |
1 |
0.4286 |
Tier II |
| 4 |
NC_000023.11 |
X |
135306253 |
+ |
ZNF75D |
NNNCTCCACAGATCCACAGA |
NGG |
2 |
0.3029 |
Tier II |
| 5 |
NC_000016.10 |
16 |
89145744 |
+ |
ACSF3 |
NNNGTCCCCAGATCCACCGT |
NGG |
2 |
0.2143 |
Tier II |
| 6 |
NC_000007.14 |
7 |
158477949 |
+ |
PTPRN2 |
NNNCTCCACACATCCACCCT |
NGG |
2 |
0.1921 |
Tier II |
| 7 |
NC_000011.10 |
11 |
116857315 |
+ |
SIK3 |
NNNCTCCACAGATACACCGT |
NAG |
2 |
0.0907 |
Tier II |
| 8 |
NC_000007.14 |
7 |
4944461 |
- |
MMD2 |
NNNCTCCACAGATCCAGCGT |
NGG |
1 |
0.0588 |
Tier II |
| 9 |
NC_000018.10 |
18 |
46523861 |
+ |
LOXHD1 |
NNNCTCCACAGATCCACCAT |
NTG |
2 |
0.0278 |
Tier II |
| 10 |
NC_000011.10 |
11 |
47467237 |
+ |
CELF1 |
NNNCTCCACAGATCCACCCT |
NTG |
2 |
0.0175 |
Tier II |
| 11 |
NC_000007.14 |
7 |
153191719 |
+ |
LOC102723686 |
NNNCTCCACAGATTCACCGA |
NGG |
2 |
0.4125 |
Tier III |
| 12 |
NC_000005.10 |
5 |
135142676 |
- |
PITX1-AS1 |
NNNCTACACAGCTCCACCGT |
NGG |
2 |
0.2444 |
Tier III |
| 13 |
NC_000007.14 |
7 |
97386516 |
+ |
LOC105375416 |
NNNGTCCACAGATACACCGT |
NGG |
2 |
0.175 |
Tier III |
| 14 |
NC_000012.12 |
12 |
87771875 |
+ |
LINC02258 |
NNNCTCCACAGATCCACCTC |
NGG |
2 |
0.0606 |
Tier III |
| 15 |
NC_000002.12 |
2 |
234830991 |
- |
LOC101927896 |
NNNCTCCAGAGATCCACCGT |
NGT |
2 |
0.01 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
58159883 |
+ |
Idnk |
NNNCTCCACAGATCCACTGT |
NGG |
1 |
0.6429 |
Tier I |
| 2 |
NC_000072.6 |
6 |
128238123 |
+ |
Tead4 |
NNNATCCACAGATCCACCTT |
NGG |
2 |
0.5614 |
Tier II |
| 3 |
NC_000079.6 |
13 |
97960746 |
+ |
Arhgef28 |
NNNATCCACAGATCCACTGT |
NGG |
2 |
0.5414 |
Tier II |
| 4 |
NC_000082.6 |
16 |
43869393 |
+ |
Qtrt2 |
NNNCTCCACAGATTCACCAT |
NGG |
2 |
0.5238 |
Tier II |
| 5 |
NC_000075.6 |
9 |
10636946 |
- |
Cntn5 |
NNNCTCCACAGATTCACTGT |
NGG |
2 |
0.4714 |
Tier II |
| 6 |
NC_000073.6 |
7 |
46554322 |
- |
Sergef |
NNNCTCCACAGACCCACCCT |
NGG |
2 |
0.3539 |
Tier II |
| 7 |
NC_000067.6 |
1 |
179416449 |
- |
Smyd3 |
NNNCTCCACACATCCACTGT |
NGG |
2 |
0.2755 |
Tier II |
| 8 |
NC_000071.6 |
5 |
116231227 |
- |
Ccdc60 |
NNNCTCCACAGATACACAGT |
NGG |
2 |
0.1885 |
Tier II |
| 9 |
NC_000079.6 |
13 |
40238173 |
+ |
Ofcc1 |
NNNCTCCACAGCACCACCGT |
NGG |
2 |
0.1822 |
Tier II |
| 10 |
NC_000077.6 |
11 |
107977550 |
- |
Prkca |
NNNCTCCACAGAGCCACTGT |
NGG |
2 |
0.1677 |
Tier II |
| 11 |
NC_000080.6 |
14 |
31137002 |
- |
Nt5dc2 |
NNNCTCCACAGGTCCACCGC |
NGG |
2 |
0.0657 |
Tier II |
| 12 |
NC_000078.6 |
12 |
88453358 |
- |
Adck1 |
NNNCTCCAGAGATCTACCGT |
NGG |
2 |
0.0413 |
Tier II |
| 13 |
NC_000067.6 |
1 |
134684069 |
- |
Syt2 |
NNNCTCCACAGATGCACCGT |
NGC |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
