Construct: sgRNA BRDN0001146517
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGCCCGCCAACAAAACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGFR1 (2260)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76069
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
38424649 |
- |
FGFR1 |
NNNGCCCGCCAACAAAACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
236369300 |
- |
IQCA1 |
NNNGCCCCACAACAAAACAG |
NGG |
2 |
0.5275 |
Tier II |
| 3 |
NC_000009.12 |
9 |
16708804 |
+ |
BNC2 |
NNNGCCCCACAACAAAACAG |
NGG |
2 |
0.5275 |
Tier II |
| 4 |
NC_000004.12 |
4 |
146895198 |
- |
TTC29 |
NNNGCCAGCCAATAAAACAG |
NGG |
2 |
0.525 |
Tier II |
| 5 |
NC_000010.11 |
10 |
104817734 |
- |
SORCS3 |
NNNGCCTGCCAACAAAAAAG |
NGG |
2 |
0.4375 |
Tier II |
| 6 |
NC_000002.12 |
2 |
43725369 |
- |
PLEKHH2 |
NNNGCCCCCCAATAAAACAG |
NGG |
2 |
0.4308 |
Tier II |
| 7 |
NC_000009.12 |
9 |
131671456 |
+ |
RAPGEF1 |
NNNGCCCGCCCACAAAACTG |
NGG |
2 |
0.2154 |
Tier II |
| 8 |
NC_000006.12 |
6 |
10162578 |
- |
OFCC1 |
NNNGCCACCCAACAAAACAG |
NGG |
2 |
0.4615 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
86641675 |
- |
Lonp2 |
NNNGCGGGCCAACAAAACAG |
NGG |
2 |
0.2353 |
Tier I |
| 2 |
NC_000071.6 |
5 |
144813426 |
- |
Trrap |
NNNGCCCGCCTACAAAACAT |
NGG |
2 |
0.2154 |
Tier I |
| 3 |
NC_000074.6 |
8 |
25562189 |
+ |
Fgfr1 |
NNNGCCTGCCAACAAGACAG |
NGG |
2 |
0.1563 |
Tier I |
| 4 |
NC_000080.6 |
14 |
69475586 |
+ |
Gm21451 |
NNNGCCCGCCCACAATACAG |
NGG |
2 |
0.0 |
Tier I |
| 5 |
NC_000068.7 |
2 |
31766663 |
- |
Abl1 |
NNNGCCTGCCAACAAAACAC |
NGG |
2 |
0.3482 |
Tier II |
| 6 |
NC_000080.6 |
14 |
69257336 |
+ |
Slc25a37 |
NNNGCCCGCCCACAATACAG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000080.6 |
14 |
69475586 |
+ |
Gm16867 |
NNNGCCCGCCCACAATACAG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
