Construct: sgRNA BRDN0001146521

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGAGTCACCTAAAGTTAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK14 (5218)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76639

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 90726789 + CDK14 NNNAGTCACCTAAAGTTAGG NGG 0 1.0 Tier I
2 NC_000007.14 7 28700590 + CREB5 NNNAGTCACACAAAGTTAGG NGG 2 0.5778 Tier II
3 NC_000010.11 10 46379563 - ANXA8L1 NNNACTCACCCAAAGTTAGG NGG 2 0.5238 Tier II
4 NC_000010.11 10 47480211 + ANXA8 NNNACTCACCCAAAGTTAGG NGG 2 0.5238 Tier II
5 NC_000011.10 11 102623901 + MMP20 NNNAGGCACCTAAAGCTAGG NGG 2 0.381 Tier II
6 NC_000012.12 12 25483021 - LMNTD1 NNNAGTACCCTAAAGTTAGG NGG 2 0.3214 Tier II
7 NC_000018.10 18 63303784 - BCL2 NNNAGTCACCGAAAGTCAGG NGG 2 0.2824 Tier II
8 NC_000001.11 1 144157459 - LOC105371206 NNNAATCACCTAAACTTAGG NGG 2 0.2364 Tier II
9 NC_000001.11 1 144808925 + LOC105371216 NNNAATCACCTAAACTTAGG NGG 2 0.2364 Tier II
10 NC_000002.12 2 87344461 + LOC124907854 NNNAATCACCTAAACTTAGG NGG 2 0.2364 Tier II
11 NC_000020.11 20 32096819 + HCK NNNAGTCACCTTAAGTAAGG NGG 2 0.1778 Tier II
12 NC_000015.10 15 34261629 + SLC12A6 NNNAGTCACCTAAGTTTAGG NGG 2 0.0667 Tier II
13 NC_000008.11 8 52275855 + ST18 NNNAGTCCCCTAAATTTAGG NGG 2 0.0612 Tier II
14 NC_000017.11 17 61726557 - BRIP1 NNNAGTCACCTAAAGTTATG NGC 2 0.0148 Tier II
15 NC_000014.9 14 40277566 - LOC105370463 NNNAGAAACCTAAAGTTAGG NGG 2 0.65 Tier III
16 NC_000024.10 Y 17555563 - LOC105377217 NNNAGCCACCTAAAGTTGGG NGG 2 0.3636 Tier III
17 NC_000024.10 Y 18350448 + LOC105377220 NNNAGCCACCTAAAGTTGGG NGG 2 0.3636 Tier III
18 NC_000001.11 1 143331886 - LOC105371175 NNNAATCACCTAAACTTAGG NGG 2 0.2364 Tier III
19 NC_000002.12 2 181295262 - LINC01934 NNNAGTCACCTAAAGCTAGG NGT 2 0.0108 Tier III
20 NC_000011.10 11 36863731 - LOC107984326 NNNAGTCACCTAAATTTAGG NGA 2 0.0099 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 5249007 - Cdk14 NNNAGTCACCTAAAGTTCGG NGG 1 0.1905 Tier I
2 NC_000067.6 1 182259886 - Gm33887 NNNAGTCACCTAAGGTTAGG NGG 1 0.4667 Tier II
3 NC_000071.6 5 76933283 - Ppat NNNAGTCACCTAAGGTTAGG NGG 1 0.4667 Tier II
4 NC_000074.6 8 90888819 - Chd9 NNNAGTCACCTAAGGTTAGG NGG 1 0.4667 Tier II
5 NC_000077.6 11 8923064 - Pkd1l1 NNNAGTCACCTAAGGTTAGG NGG 1 0.4667 Tier II
6 NC_000077.6 11 8923064 - Gm40814 NNNAGTCACCTAAGGTTAGG NGG 1 0.4667 Tier II
7 NC_000078.6 12 54776991 - Snx6 NNNAGTCACCTAAGGTTAGG NGG 1 0.4667 Tier II
8 NC_000068.7 2 146479061 + Ralgapa2 NNNAGTCACCTGAAGTTTGG NGG 2 0.3611 Tier II
9 NC_000082.6 16 18910871 + Hira NNNAGTCACAGAAAGTTAGG NGG 2 0.3467 Tier II
10 NC_000067.6 1 131645384 - Ctse NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
11 NC_000068.7 2 16026727 + Malrd1 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
12 NC_000068.7 2 156361026 + Cnbd2 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
13 NC_000069.6 3 67098889 - Rsrc1 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
14 NC_000069.6 3 152325950 - Miga1 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
15 NC_000070.6 4 134162022 - Cep85 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
16 NC_000070.6 4 146505749 - Zfp981 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
17 NC_000070.6 4 147221468 - Zfp978 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
18 NC_000071.6 5 25233508 - Galnt11 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
19 NC_000075.6 9 62441815 - Coro2b NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
20 NC_000077.6 11 6008549 - Camk2b NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
21 NC_000077.6 11 76551619 - Abr NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
22 NC_000077.6 11 103086473 - Plcd3 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
23 NC_000085.6 19 60931384 + Grk5 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier II
24 NC_000067.6 1 14899548 + Trpa1 NNNAGTCAACTATAGTTAGG NGG 2 0.2571 Tier II
25 NC_000086.7 X 87432110 - Il1rapl1 NNNAGTCACCAAAAGTTAGG NAG 2 0.1944 Tier II
26 NC_000078.6 12 117667963 + Rapgef5 NNNAGTCCCCTTAAGTTAGG NGG 2 0.1429 Tier II
27 NC_000076.6 10 69274299 - Rhobtb1 NNNAGTCACCTCAGGTTAGG NGG 2 0.1228 Tier II
28 NC_000067.6 1 10405626 - Cpa6 NNNAGTCACCTAAACTTGGG NGG 2 0.1091 Tier II
29 NC_000081.6 15 10335910 + Prlr NNNAGTCACCTCAAGTTGGG NGG 2 0.1053 Tier II
30 NC_000071.6 5 103027846 - Mapk10 NNNAGTCACCTACATTTAGG NGG 2 0.0301 Tier II
31 NC_000067.6 1 191936379 - Gm39743 NNNAGTCACCTAAGGTTAGG NGG 1 0.4667 Tier III
32 NC_000071.6 5 76933283 - LOC115486295 NNNAGTCACCTAAGGTTAGG NGG 1 0.4667 Tier III
33 NC_000082.6 16 76141258 + 4930578N18Rik NNNAGTCACCTAAGGTTAGG NGG 1 0.4667 Tier III
34 NC_000068.7 2 16026727 + Gm37416 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier III
35 NC_000070.6 4 146505749 - Vmn2r-ps16 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier III
36 NC_000071.6 5 24217920 - Gm43828 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier III
37 NC_000071.6 5 43210750 - Gm7854 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier III
38 NC_000074.6 8 85129592 + Gm42031 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier III
39 NC_000078.6 12 19245904 - Gm40843 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier III
40 NC_000080.6 14 54295256 + Gm43305 NNNAGTCACCTAAGGTCAGG NGG 2 0.3294 Tier III
41 NC_000084.6 18 47993417 + G630055G22Rik NNNAGTCACCTAATGTTTGG NGG 2 0.2667 Tier III
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Other clones with same target sequence:

(none)