GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001146524

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: GCTGATTGGACGAGCCGGAG

Original Target:

Taxon:: Homo sapiens (human)
Gene:: SRPK3 (26576)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 77395

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000023.11	X	153784167	-	SRPK3	NNNGATTGGACGAGCCGGAG	NGG	0	1.0	Tier I
2	NC_000023.11	X	153784167	-	LOC124905227	NNNGATTGGACGAGCCGGAG	NGG	0	1.0	Tier III
3	NC_000019.10	19	54533277	-	KIR3DX1	NNNGATTGGATGAGCCTGAG	NGG	2	0.0769	Tier III
4	NC_000012.12	12	17662849	+	LOC124902889	NNNGATTGGACGAGCTGGAG	NTG	2	0.012	Tier III

Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000069.6	3	67314635	-	Rsrc1	NNNGATTGAACGAGCCAGAG	NGG	2	0.6	Tier II
2	NC_000071.6	5	37136214	-	Jakmip1	NNNGATTGGAGGAGCCAGAG	NGG	2	0.2333	Tier II
3	NC_000076.6	10	32837581	+	Nkain2	NNNGATTGGAGGAGCCAGAG	NGG	2	0.2333	Tier II
4	NC_000077.6	11	43124181	+	Atp10b	NNNGATTGGAGGAGCCAGAG	NGG	2	0.2333	Tier II
5	NC_000080.6	14	34389919	-	Mmrn2	NNNGATTGGAGGAGCCAGAG	NGG	2	0.2333	Tier II
6	NC_000085.6	19	4215382	+	Clcf1	NNNGAGTGGACGCGCCGGAG	NGG	2	0.1203	Tier II
7	NC_000068.7	2	51690516	-	Gm13490	NNNGATTAGAAGAGCCGGAG	NGG	2	0.75	Tier III
8	NC_000076.6	10	87783628	+	Gm30073	NNNGATTGGAAGAGCCAGAG	NGG	2	0.7	Tier III
9	NC_000077.6	11	42864286	+	Gm34644	NNNGATTGGAAGAGCCAGAG	NGG	2	0.7	Tier III
10	NC_000072.6	6	115509008	+	Gm36301	NNNGATTGTACGAGCCAGAG	NGG	2	0.4978	Tier III
11	NC_000072.6	6	115514503	+	Gm36301	NNNGATTGTAAGAGCCGGAG	NGG	2	0.4	Tier III
12	NC_000068.7	2	51083871	+	Gm32360	NNNGATTGGAGGAGCCAGAG	NGG	2	0.2333	Tier III
13	NC_000069.6	3	62680130	-	Gm34048	NNNGATTGGAGGAGCCAGAG	NGG	2	0.2333	Tier III
14	NC_000079.6	13	90930852	+	Gm46434	NNNGATTGGAGGAGCCAGAG	NGG	2	0.2333	Tier III
15	NC_000085.6	19	5523114	-	Gm31166	NNNGATTGTAGGAGCCGGAG	NGG	2	0.1333	Tier III
16	NC_000085.6	19	4215382	+	LOC102631992	NNNGAGTGGACGCGCCGGAG	NGG	2	0.1203	Tier III
17	NC_000085.6	19	4215382	+	Gm45928	NNNGAGTGGACGCGCCGGAG	NGG	2	0.1203	Tier III
18	NC_000080.6	14	109506527	-	Gm30216	NNNGATTGGAGGAGCTGGAG	NGG	2	0.0769	Tier III

Download CSV

Other clones with same target sequence:

(none)