Construct: sgRNA BRDN0001146526
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGATTCGGAGCCTTGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAA1 (5562)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76253
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
40777515 |
- |
PRKAA1 |
NNNGATTCGGAGCCTTGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
22729534 |
+ |
PEBP4 |
NNNGATCTGGAGCCTTGATG |
NGG |
2 |
0.6016 |
Tier II |
| 3 |
NC_000005.10 |
5 |
40777515 |
- |
LOC124900968 |
NNNGATTCGGAGCCTTGATG |
NGG |
0 |
1.0 |
Tier III |
| 4 |
NC_000021.9 |
21 |
27235898 |
+ |
LOC102724355 |
NNNGATTCAGAGCCTAGATG |
NGG |
2 |
0.5844 |
Tier III |
| 5 |
NC_000008.11 |
8 |
127795048 |
+ |
PVT1 |
NNNGATTTGGAGCCATGATG |
NGG |
2 |
0.5066 |
Tier III |
| 6 |
NC_000018.10 |
18 |
49028273 |
+ |
DYM-AS1 |
NNNGATTCGGTGCCATGATG |
NGG |
2 |
0.1781 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
5160675 |
+ |
Prkaa1 |
NNNGATTCGGAGCCTTGACG |
NGG |
1 |
0.2759 |
Tier I |
| 2 |
NC_000074.6 |
8 |
13630212 |
- |
Rasa3 |
NNNGATTTGGAGCCTAGATG |
NGG |
2 |
0.7955 |
Tier II |
| 3 |
NC_000068.7 |
2 |
174680385 |
+ |
Zfp831 |
NNNGGTTGGGAGCCTTGATG |
NGG |
2 |
0.4629 |
Tier II |
| 4 |
NC_000081.6 |
15 |
58621040 |
+ |
Fer1l6 |
NNNGATTGTGAGCCTTGATG |
NGG |
2 |
0.3429 |
Tier II |
| 5 |
NC_000071.6 |
5 |
93100632 |
+ |
Septin11 |
NNNGATTCTGAGCCTGGATG |
NGG |
2 |
0.1846 |
Tier II |
| 6 |
NC_000071.6 |
5 |
49273853 |
+ |
Kcnip4 |
NNNGATTCTGAGCCTTGATG |
NAG |
2 |
0.1383 |
Tier II |
| 7 |
NC_000082.6 |
16 |
13406955 |
- |
Mrtfb |
NNNGATTCTGAGCCTTTATG |
NGG |
2 |
0.1333 |
Tier II |
| 8 |
NC_000076.6 |
10 |
42866853 |
- |
Scml4 |
NNNGATTCGGAGCCCTGCTG |
NGG |
2 |
0.0519 |
Tier II |
| 9 |
NC_000070.6 |
4 |
94113724 |
- |
Gm12648 |
NNNGATTTGGAGCCTTGCTG |
NGG |
2 |
0.1667 |
Tier III |
| 10 |
NC_000082.6 |
16 |
13406955 |
- |
Gm15738 |
NNNGATTCTGAGCCTTTATG |
NGG |
2 |
0.1333 |
Tier III |
| 11 |
NC_000076.6 |
10 |
42866853 |
- |
Gm34006 |
NNNGATTCGGAGCCCTGCTG |
NGG |
2 |
0.0519 |
Tier III |
Other clones with same target sequence:
(none)
