Construct: sgRNA BRDN0001146530

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAAGATAAACCATTAGATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2H1 (2965)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76599

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 18341603 + GTF2H1 NNNAGATAAACCATTAGATG NGG 0 1.0 Tier I
2 NC_000001.11 1 21839996 - HSPG2 NNNAGCTAAACCATCAGATG NGG 2 0.124 Tier I
3 NC_000011.10 11 83994095 - DLG2 NNNAGATAAACCGTTAGATG NGG 1 0.6522 Tier II
4 NC_000005.10 5 59174743 - PDE4D NNNAAATAAACCATTAGATT NGG 2 0.6067 Tier II
5 NC_000014.9 14 47663740 - MDGA2 NNNGGATAAACCATTAAATG NGG 2 0.5833 Tier II
6 NC_000008.11 8 55205709 + XKR4 NNNTGATAAAACATTAGATG NGG 2 0.4773 Tier II
7 NC_000016.10 16 11038628 - CLEC16A NNNAGATAAAATATTAGATG NGG 2 0.4038 Tier II
8 NC_000004.12 4 39357534 - RFC1 NNNAAATAAACCATTAGATC NGG 2 0.3714 Tier II
9 NC_000011.10 11 18022022 - TPH1 NNNAGAGAAACCATTAGTTG NGG 2 0.2941 Tier II
10 NC_000006.12 6 88657952 - RNGTT NNNAGAAAAACCATCAGATG NGG 2 0.2386 Tier II
11 NC_000018.10 18 12489769 + SPIRE1 NNNAAATAAACCATTAGATG NAG 2 0.2247 Tier II
12 NC_000006.12 6 169692827 - WDR27 NNNAGACAAACCATCAGATG NGG 2 0.1875 Tier II
13 NC_000006.12 6 57566357 - PRIM2 NNNAGACAAACCATTAGATG NAG 2 0.1782 Tier II
14 NC_000017.11 17 61337380 + BCAS3 NNNAAATAAACCATTAGCTG NGG 2 0.1651 Tier II
15 NC_000005.10 5 109707602 + MAN2A1 NNNAGATAAACCATAACATG NGG 2 0.1362 Tier II
16 NC_000019.10 19 47350173 + DHX34 NNNAGATAAAGTATTAGATG NGG 2 0.1346 Tier II
17 NC_000003.12 3 78974706 - ROBO1 NNNAGAAAAACCATTAGATG NGA 2 0.0608 Tier II
18 NC_000013.11 13 74024357 - KLF12 NNNACATAAACCATTAGATG NGC 2 0.0175 Tier II
19 NC_000023.11 X 12176836 + FRMPD4 NNNAGGTAAACCATTAGATG NGT 2 0.0115 Tier II
20 NC_000002.12 2 79947573 + CTNNA2 NNNAGATAAACCATTAGATT NGT 2 0.0113 Tier II
21 NC_000006.12 6 77886868 - MEI4 NNNAGATAAACCATTAGCTG NGC 2 0.0042 Tier II
22 NC_000004.12 4 163161832 + NAF1 NNNAGATAAACCATTCCATG NGG 2 0.0 Tier II
23 NC_000002.12 2 44628934 - CAMKMT NNNAGATAAACCATTTGATG NGC 2 0.0 Tier II
24 NC_000018.10 18 903951 - LOC124904340 NNNAGATAGACCATTAAATG NGG 2 0.6222 Tier III
25 NC_000003.12 3 18154693 - BALR6 NNNAAATAAACAATTAGATG NGG 2 0.619 Tier III
26 NC_000005.10 5 56309997 + LOC105378977 NNNAGATAATCCATTAGATT NGG 2 0.6176 Tier III
27 NC_000018.10 18 60197383 + LOC342784 NNNAAATAAGCCATTAGATG NGG 2 0.4815 Tier III
28 NC_000010.11 10 79765708 + NUTM2B-AS1 NNNAGATAAGCAATTAGATG NGG 2 0.3968 Tier III
29 NC_000015.10 15 93241353 - LOC105370982 NNNAGCCAAACCATTAGATG NGG 2 0.3125 Tier III
30 NC_000005.10 5 29149977 - LINC02109 NNNACATAAACCATCAGATG NGG 2 0.2143 Tier III
31 NC_000003.12 3 91455286 + LOC101930420 NNNAGACAAACCATTAGATG NAG 2 0.1782 Tier III
32 NC_000001.11 1 146503220 - HYDIN2 NNNAGATAAATTATTAGATG NGG 2 0.1657 Tier III
33 NC_000001.11 1 148230174 + LOC101927468 NNNAGATAAATTATTAGATG NGG 2 0.1657 Tier III
34 NC_000012.12 12 117104862 - TESC-AS1 NNNAGCTAAACCATTAGAAG NGG 2 0.1299 Tier III
35 NC_000003.12 3 186473249 - LINC02052 NNNAGATAAACCATTACATG NGA 2 0.0163 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 46808875 + Gtf2h1 NNNAGATAAGCCATTAGACG NGG 2 0.1533 Tier I
2 NC_000072.6 6 96443738 - Tafa1 NNNAGATATACCATTAAATG NGG 2 0.56 Tier II
3 NC_000085.6 19 57294422 - Ablim1 NNNAGATAAGCCATTAAATG NGG 2 0.5185 Tier II
4 NC_000069.6 3 65127411 - Kcnab1 NNNGGATAAAACATTAGATG NGG 2 0.4688 Tier II
5 NC_000080.6 14 122684622 + Pcca NNNAGCTAAACCATTAAATG NGG 2 0.4242 Tier II
6 NC_000076.6 10 40137510 - Slc16a10 NNNAAATCAACCATTAGATG NGG 2 0.3714 Tier II
7 NC_000073.6 7 90148657 + Picalm NNNAAATAAATCATTAGATG NGG 2 0.2667 Tier II
8 NC_000082.6 16 74124143 + Robo2 NNNAGATAAACCATTAGAAT NGG 2 0.2 Tier II
9 NC_000068.7 2 102396601 + Trim44 NNNAGATAAACCATTAGATT NAG 2 0.1815 Tier II
10 NC_000073.6 7 112823677 - Tead1 NNNAGATAAACAATTAGCTG NGG 2 0.1361 Tier II
11 NC_000072.6 6 50208445 - Gsdme NNNAGATAAACCCTTACATG NGG 2 0.0495 Tier II
12 NC_000070.6 4 42203017 + Ccl21b NNNAGAGAAACCATGAGATG NGG 2 0.0294 Tier II
13 NT_187054.1 4 47973 + LOC108168680 NNNAGAGAAACCATGAGATG NGG 2 0.0294 Tier II
14 NC_000084.6 18 9744312 - Colec12 NNNAGATAAACCACTTGATG NGG 2 0.0 Tier II
15 NC_000080.6 14 124181713 + Fgf14 NNNAGATAAATCATTTGATG NGG 2 0.0 Tier II
16 NC_000081.6 15 74933016 + Gm39556 NNNAGCTAAACCATTAGATG NGG 1 0.4545 Tier III
17 NC_000067.6 1 35953532 + Gm33176 NNNAGATGAACCATTAGTTG NGG 2 0.3667 Tier III
18 NC_000083.6 17 9530967 - Gm51424 NNNAGATAAACCATCAGATA NGG 2 0.2557 Tier III
19 NC_000085.6 19 15640603 + Gm46665 NNNAGATAAACATTTAGATG NGG 2 0.2143 Tier III
20 NC_000070.6 4 42700164 + 4930578G10Rik NNNAGAGAAACCATGAGATG NGG 2 0.0294 Tier III
21 NC_000080.6 14 53629810 - Tcra NNNAGATAAACCATTAGATA NGC 2 0.0208 Tier III
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Other clones with same target sequence:

(none)