Construct: sgRNA BRDN0001146533

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGTGGAAGGCCATGACCGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NADK (65220)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78071

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 1755463 + NADK NNNGGAAGGCCATGACCGGA NGG 0 1.0 Tier I
2 NC_000004.12 4 139168976 + ELF2 NNNGGAAGGCCATGAAAGGA NGG 2 0.4667 Tier II
3 NC_000014.9 14 96366665 + GSKIP NNNGGAAGGCCAAGGCCGGA NGG 2 0.45 Tier II
4 NC_000022.11 22 46298721 - GTSE1 NNNGGAAGGACATGACAGGA NGG 2 0.4044 Tier II
5 NC_000010.11 10 21565852 - MLLT10 NNNGGGAGGCCATGACAGGA NGG 2 0.3333 Tier II
6 NC_000007.14 7 133669187 + EXOC4 NNNGGGAGGCCATGACTGGA NGG 2 0.3333 Tier II
7 NC_000002.12 2 61452656 + USP34 NNNGGAAGGCCAAGACAGGA NGG 2 0.3231 Tier II
8 NC_000012.12 12 69747551 - RAB3IP NNNGGAAGGCCAAGACAGGA NGG 2 0.3231 Tier II
9 NC_000021.9 21 39319001 - BRWD1 NNNGGAAGGCCAAGACAGGA NGG 2 0.3231 Tier II
10 NC_000008.11 8 72775165 - KCNB2 NNNGGAAGGCCCTGACCGGA NGG 1 0.2632 Tier II
11 NC_000016.10 16 24883962 - SLC5A11 NNNGGAAGGCCAGGACCCGA NGG 2 0.1242 Tier II
12 NC_000002.12 2 234075652 - SPP2 NNNGGAAGGCCATGAGCAGA NGG 2 0.1065 Tier II
13 NC_000001.11 1 147290623 - CHD1L NNNGGAAGGCCATGAAGGGA NGG 2 0.0588 Tier II
14 NC_000005.10 5 173133577 - CREBRF NNNGGAAGGCCATGAAGGGA NGG 2 0.0588 Tier II
15 NC_000009.12 9 5819356 + ERMP1 NNNGGAAGGCCATGAAGGGA NGG 2 0.0588 Tier II
16 NC_000009.12 9 69616939 + APBA1 NNNGGAAGGCCATGAAGGGA NGG 2 0.0588 Tier II
17 NC_000023.11 X 104845696 - IL1RAPL2 NNNGGAAGGCCATGAAGGGA NGG 2 0.0588 Tier II
18 NC_000008.11 8 1865798 - ARHGEF10 NNNGGAAGGCCAAGACCGGA NGC 2 0.0154 Tier II
19 NC_000009.12 9 132964528 + GFI1B NNNGGAAGGCCATGACGGGC NGG 2 0.0134 Tier II
20 NC_000004.12 4 72070666 - NPFFR2 NNNGGAAGGCCATGAGGGGA NGG 2 0.009 Tier II
21 NC_000002.12 2 109408929 + RANBP2 NNNGGAAGGCCATGAGCGGA NGC 2 0.0034 Tier II
22 NC_000002.12 2 109408929 + SH3RF3 NNNGGAAGGCCATGAGCGGA NGC 2 0.0034 Tier II
23 NC_000006.12 6 134734013 + LOC101928277 NNNGGAAGGCCATGAAAGGA NGG 2 0.4667 Tier III
24 NC_000021.9 21 39319001 - BRWD1-AS1 NNNGGAAGGCCAAGACAGGA NGG 2 0.3231 Tier III
25 NC_000013.11 13 73547008 + LINC00393 NNNTGAAGGCCATGACCAGA NGG 2 0.2517 Tier III
26 NC_000004.12 4 174381588 - LOC105377547 NNNGGAAGGCCATGAAGGGA NGG 2 0.0588 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 81967695 - Asic2 NNNGGTAGGCCATGACCTGA NGG 2 0.4762 Tier I
2 NC_000070.6 4 155586743 - Nadk NNNGGAAGGCCATGACAGGA NGC 2 0.0104 Tier I
3 NC_000084.6 18 84439106 - Zfp407 NNNGGAAGGTCATGACCTGA NGG 2 0.6275 Tier II
4 NC_000079.6 13 43365717 + Sirt5 NNNGGAAGGCAGTGACCGGA NGG 2 0.5417 Tier II
5 NC_000080.6 14 70124557 - Bin3 NNNGGAAGGCCATGCCCGGA NGG 1 0.2727 Tier II
6 NC_000067.6 1 131325302 + Srgap2 NNNGGAAGGCCATGATCTGA NGG 2 0.2051 Tier II
7 NC_000071.6 5 151288152 - Stard13 NNNGGACGGCCATGACAGGA NGG 2 0.2042 Tier II
8 NC_000071.6 5 125062626 + Ncor2 NNNGTAAGGCCATGACAGGA NGG 2 0.14 Tier II
9 NC_000086.7 X 6632022 - Shroom4 NNNGGAAGGACATGAGCGGA NGG 2 0.1333 Tier II
10 NC_000067.6 1 131325302 + Gm51644 NNNGGAAGGCCATGATCTGA NGG 2 0.2051 Tier III
11 NC_000081.6 15 97172748 - Gm33149 NNNGCAAGGCCATGTCCGGA NGG 2 0.1571 Tier III
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Other clones with same target sequence:

(none)