Construct: sgRNA BRDN0001146535

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACGTTATTACAGAGCCCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK10 (5602)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75982

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 86101088 - MAPK10 NNNTTATTACAGAGCCCCTG NGG 0 1.0 Tier I
2 NC_000018.10 18 62978218 - PHLPP1 NNNTTAATACAGAGCACCTG NGG 2 0.875 Tier II
3 NC_000021.9 21 33761783 + ITSN1 NNNTTTTTACAAAGCCCCTG NGG 2 0.6667 Tier II
4 NC_000010.11 10 18327195 + CACNB2 NNNTTATTCCAGAGCACCTG NGG 2 0.5714 Tier II
5 NC_000002.12 2 235529165 + AGAP1 NNNTTGTGACAGAGCCCCTG NGG 2 0.5238 Tier II
6 NC_000010.11 10 120544322 + PLPP4 NNNTTTTGACAGAGCCCCTG NGG 2 0.5238 Tier II
7 NC_000005.10 5 135825822 + SLC25A48 NNNTTACTACAGAACCCCTG NGG 2 0.5156 Tier II
8 NC_000016.10 16 53260581 + CHD9 NNNTTATTACAAAGCCCATG NGG 2 0.5026 Tier II
9 NC_000005.10 5 168757245 + SLIT3 NNNTTAGTTCAGAGCCCCTG NGG 2 0.3529 Tier II
10 NC_000001.11 1 41750194 - HIVEP3 NNNTCATTACAGAGACCCTG NGG 2 0.2222 Tier II
11 NC_000004.12 4 134126513 + PABPC4L NNNTTATTAAAGAGACCCTG NGG 2 0.1926 Tier II
12 NC_000011.10 11 111923640 - CRYAB NNNTTATTTCTGAGCCCCTG NGG 2 0.1846 Tier II
13 NC_000011.10 11 111923640 - C11orf52 NNNTTATTTCTGAGCCCCTG NGG 2 0.1846 Tier II
14 NC_000020.11 20 43066936 + PTPRT NNNTTATGACAGAGACCCTG NGG 2 0.163 Tier II
15 NC_000014.9 14 73343189 + NUMB NNNTTATTACAGAGCCACTG NAG 2 0.121 Tier II
16 NC_000005.10 5 145979975 + SH3RF2 NNNTTATTCCAGAGCCCGTG NGG 2 0.0762 Tier II
17 NC_000012.12 12 54558653 - PDE1B NNNTTATTCCAGAGGCCCTG NGG 2 0.0286 Tier II
18 NC_000012.12 12 85297731 + ALX1 NNNTTAATACAGAGCCCCTG NGC 2 0.0194 Tier II
19 NC_000001.11 1 169726043 - SELE NNNTTATTACAGAGGCCCTG NAG 2 0.013 Tier II
20 NC_000010.11 10 97170074 - SLIT1 NNNTTATTACAGAGCGCCTG NGC 2 0.0034 Tier II
21 NC_000010.11 10 103665952 + SH3PXD2A NNNTTATTACAGAGCCCCTG NCT 2 0.0 Tier II
22 NC_000005.10 5 135825822 + LOC107986453 NNNTTACTACAGAACCCCTG NGG 2 0.5156 Tier III
23 NC_000018.10 18 22759789 - RBBP8-AS1 NNNTTATTAAAGAGCCCATG NGG 2 0.4667 Tier III
24 NC_000011.10 11 111923640 - HSPB2-C11orf52 NNNTTATTTCTGAGCCCCTG NGG 2 0.1846 Tier III
25 NC_000002.12 2 12172529 + MIR3681HG NNNCTATTACAGAGACCCTG NGG 2 0.1438 Tier III
26 NC_000005.10 5 145979975 + LOC107986458 NNNTTATTCCAGAGCCCGTG NGG 2 0.0762 Tier III
27 NC_000006.12 6 74168020 - LOC101928516 NNNTTATCACAGAGGCCCTG NGG 2 0.05 Tier III
28 NC_000004.12 4 144233862 + LOC105377462 NNNTTATTAAAGAGCCCCTG NGT 2 0.014 Tier III
29 NC_000010.11 10 97170074 - ARHGAP19-SLIT1 NNNTTATTACAGAGCGCCTG NGC 2 0.0034 Tier III
30 NC_000010.11 10 103665952 + LOC124902495 NNNTTATTACAGAGCCCCTG NCT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 102989656 - Mapk10 NNNATATTACAGAGCCCCTG NGG 1 0.4762 Tier I
2 NC_000068.7 2 121163752 - Zscan29 NNNTTATTCCAGAGCCCCAG NGG 2 0.1633 Tier I
3 NC_000081.6 15 26756796 - Fbxl7 NNNTTATTTCAGAGCACCTG NGG 2 0.6 Tier II
4 NC_000077.6 11 113639059 - Slc39a11 NNNTTCTAACAGAGCCCCTG NGG 2 0.3636 Tier II
5 NC_000074.6 8 16465966 + Csmd1 NNNTTGTTACAGAGCCACTG NGG 2 0.3333 Tier II
6 NC_000080.6 14 39445699 - Nrg3 NNNTTATTGCAGAGCCTCTG NGG 2 0.3111 Tier II
7 NC_000080.6 14 39445699 - Gm31606 NNNTTATTGCAGAGCCTCTG NGG 2 0.3111 Tier II
8 NC_000073.6 7 112776991 - Tead1 NNNTTATTACAGAGACCCTG NGG 1 0.2222 Tier II
9 NC_000075.6 9 111687672 - Stac NNNTTATTACAGTGCCCATG NGG 2 0.1615 Tier II
10 NC_000068.7 2 145486402 + Slc24a3 NNNTTATTTCAGAGACCCTG NGG 2 0.1333 Tier II
11 NC_000076.6 10 42573248 - Nr2e1 NNNTTATTTCAGAGACCCTG NGG 2 0.1333 Tier II
12 NC_000068.7 2 169755453 + Tshz2 NNNTTATTACAGAGACTCTG NGG 2 0.1037 Tier II
13 NC_000081.6 15 59502429 + Nsmce2 NNNTCATTACAGAGCCCCTG NTG 2 0.039 Tier II
14 NC_000084.6 18 75692715 + Ctif NNNTTATTTCAGAGGCCCTG NGG 2 0.03 Tier II
15 NC_000072.6 6 33955834 + Exoc4 NNNTTAGTACAGAGGCCCTG NGG 2 0.0294 Tier II
16 NC_000067.6 1 172292993 + Atp1a2 NNNTTATTACAGAGCCACTG NTG 2 0.0182 Tier II
17 NC_000070.6 4 86892555 + Acer2 NNNTTATTACAGAGCCCTTG NGT 2 0.0104 Tier II
18 NC_000071.6 5 63864090 - 0610040J01Rik NNNTTATTACAGAGCCCCGG NTG 2 0.0097 Tier II
19 NC_000074.6 8 16465966 + Gm16347 NNNTTGTTACAGAGCCACTG NGG 2 0.3333 Tier III
20 NC_000081.6 15 88429449 - Gm35831 NNNTTAGTACAGAGCTCCTG NGG 2 0.181 Tier III
21 NC_000085.6 19 56101292 + Gm52387 NNNTTATTACAGTGCTCCTG NGG 2 0.0923 Tier III
22 NC_000068.7 2 70554501 + Gad1os NNNTTCTTACAGAGCCCCTG NGA 2 0.0316 Tier III
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Other clones with same target sequence:

(none)