Construct: sgRNA BRDN0001146537
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGATCCAGACCCAGACCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKD1 (5587)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77228
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
29636315 |
- |
PRKD1 |
NNNTCCAGACCCAGACCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
19724626 |
+ |
GP1BB |
NNNTGCAGGCCCAGACCACG |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000019.10 |
19 |
10163657 |
- |
DNMT1 |
NNNTCCTGACACAGACCACG |
NGG |
2 |
0.3281 |
Tier II |
| 4 |
NC_000002.12 |
2 |
240521323 |
+ |
ANKMY1 |
NNNTCCAGACCCTGACCACT |
NGG |
2 |
0.21 |
Tier II |
| 5 |
NC_000022.11 |
22 |
41914818 |
- |
SHISA8 |
NNNTCCAGACCCAGCCCACT |
NGG |
2 |
0.1909 |
Tier II |
| 6 |
NC_000002.12 |
2 |
102525498 |
- |
SLC9A4 |
NNNTCCAGACCCAGACCCCA |
NGG |
2 |
0.1786 |
Tier II |
| 7 |
NC_000013.11 |
13 |
98729782 |
+ |
SLC15A1 |
NNNTCCCGACCCTGACCACG |
NGG |
2 |
0.1313 |
Tier II |
| 8 |
NC_000022.11 |
22 |
19724626 |
+ |
SEPT5-GP1BB |
NNNTGCAGGCCCAGACCACG |
NGG |
2 |
0.4 |
Tier III |
| 9 |
NC_000001.11 |
1 |
83822222 |
+ |
LOC101927560 |
NNNTCCAGACACAGACCAAG |
NGG |
2 |
0.3214 |
Tier III |
| 10 |
NC_000001.11 |
1 |
83822222 |
+ |
LINC01725 |
NNNTCCAGACACAGACCAAG |
NGG |
2 |
0.3214 |
Tier III |
| 11 |
NC_000006.12 |
6 |
40965894 |
+ |
LOC101929555 |
NNNTCCAGGCCCAGACCATG |
NGG |
2 |
0.3077 |
Tier III |
| 12 |
NC_000023.11 |
X |
102571352 |
- |
NXF4 |
NNNTCCATACCCAGACCACC |
NGG |
2 |
0.2679 |
Tier III |
| 13 |
NC_000017.11 |
17 |
75078708 |
+ |
TRIM80P |
NNNTCCAGAACCAGCCCACG |
NGG |
2 |
0.2364 |
Tier III |
| 14 |
NC_000015.10 |
15 |
77891007 |
+ |
CSPG4P13 |
NNNTCCAGACCCAGCCCACC |
NGG |
2 |
0.1169 |
Tier III |
| 15 |
NC_000002.12 |
2 |
28386769 |
- |
FOSL2-AS1 |
NNNTCCAGGCCCAGACCAGG |
NGG |
2 |
0.0833 |
Tier III |
| 16 |
NC_000002.12 |
2 |
28386769 |
- |
LOC105374383 |
NNNTCCAGGCCCAGACCAGG |
NGG |
2 |
0.0833 |
Tier III |
| 17 |
NC_000009.12 |
9 |
78358758 |
- |
LOC107987083 |
NNNTCCAGACCCAGACCATG |
NGA |
2 |
0.0321 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
76116621 |
- |
Pde11a |
NNNTCCAGACCCAAACCACC |
NGG |
2 |
0.3214 |
Tier II |
| 2 |
NC_000082.6 |
16 |
43605730 |
+ |
Zbtb20 |
NNNTCCAGACCCACACCACT |
NGG |
2 |
0.3 |
Tier II |
| 3 |
NC_000075.6 |
9 |
42871764 |
- |
Grik4 |
NNNTACAGACCCAGACCACC |
NGG |
2 |
0.2449 |
Tier II |
| 4 |
NC_000072.6 |
6 |
88004492 |
+ |
Rab7 |
NNNTCCATAGCCAGACCACG |
NGG |
2 |
0.2431 |
Tier II |
| 5 |
NC_000069.6 |
3 |
115954773 |
- |
Slc30a7 |
NNNTCCAGACCCAGAGCACA |
NGG |
2 |
0.1442 |
Tier II |
| 6 |
NC_000083.6 |
17 |
34627327 |
- |
Ppt2 |
NNNTCCAGACACAGACCAGG |
NGG |
2 |
0.0938 |
Tier II |
| 7 |
NC_000080.6 |
14 |
47035209 |
- |
Samd4 |
NNNTGCAGACCCAGACCAGG |
NGG |
2 |
0.075 |
Tier II |
| 8 |
NC_000069.6 |
3 |
116290331 |
- |
Cdc14a |
NNNTCCAGAGCCAGAGCACG |
NGG |
2 |
0.0598 |
Tier II |
| 9 |
NC_000077.6 |
11 |
87443066 |
- |
Tex14 |
NNNTCCAGACCCCGTCCACG |
NGG |
2 |
0.0421 |
Tier II |
| 10 |
NC_000077.6 |
11 |
87448456 |
- |
Tex14 |
NNNTCCAGACCCCGTCCACG |
NGG |
2 |
0.0421 |
Tier II |
| 11 |
NC_000077.6 |
11 |
87462115 |
- |
Tex14 |
NNNTCCAGACCCCGTCCACG |
NGG |
2 |
0.0421 |
Tier II |
| 12 |
NC_000077.6 |
11 |
87471197 |
- |
Tex14 |
NNNTCCAGACCCCGTCCACG |
NGG |
2 |
0.0421 |
Tier II |
| 13 |
NC_000077.6 |
11 |
119421179 |
- |
Rnf213 |
NNNTCCAGACCCAGACCATG |
NTG |
2 |
0.018 |
Tier II |
| 14 |
NC_000081.6 |
15 |
3565815 |
+ |
Ghr |
NNNTCAAGACCCAGACCACG |
NGT |
2 |
0.015 |
Tier II |
| 15 |
NC_000069.6 |
3 |
116136678 |
+ |
5330425B07Rik |
NNNTGCAGACCCAGACCACC |
NGG |
2 |
0.2571 |
Tier III |
| 16 |
NC_000077.6 |
11 |
87443066 |
- |
LOC115487798 |
NNNTCCAGACCCCGTCCACG |
NGG |
2 |
0.0421 |
Tier III |
Other clones with same target sequence:
(none)
