Construct: sgRNA BRDN0001146540

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTTGTAGTGGAAAAAAACG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K20 (51776)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75597

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 173198108 + MAP3K20 NNNTGTAGTGGAAAAAAACG NGG 0 1.0 Tier I
2 NC_000003.12 3 65968519 + MAGI1 NNNTGAAGTGGAAAAAAACA NGG 2 0.8125 Tier II
3 NC_000007.14 7 7779602 - UMAD1 NNNTGAAGAGGAAAAAAACG NGG 2 0.8048 Tier II
4 NC_000014.9 14 96232281 + BDKRB2 NNNTATAGTTGAAAAAAACG NGG 2 0.7042 Tier II
5 NC_000023.11 X 75415563 + ZDHHC15 NNNTGTAGTGGAAAAAAACT NGG 1 0.7 Tier II
6 NC_000004.12 4 168184811 - ANXA10 NNNTGTAGAGGAAAAAAACT NGG 2 0.65 Tier II
7 NC_000012.12 12 50668427 - DIP2B NNNCGTAGTGGAAAAAAACA NGG 2 0.6066 Tier II
8 NC_000010.11 10 24984688 - THNSL1 NNNTGTAGGGGAAAAAAACA NGG 2 0.5804 Tier II
9 NC_000010.11 10 24984688 - ENKUR NNNTGTAGGGGAAAAAAACA NGG 2 0.5804 Tier II
10 NC_000021.9 21 37067671 - PIGP NNNTGTAATGGAAAAAAATG NGG 2 0.4615 Tier II
11 NC_000006.12 6 162552293 + PRKN NNNTGTAGGGGGAAAAAACG NGG 2 0.4471 Tier II
12 NC_000009.12 9 124020051 + LHX2 NNNTGTAGTGGAAGAAAACA NGG 2 0.4375 Tier II
13 NC_000004.12 4 140098003 - MAML3 NNNCGTAGTGGAGAAAAACG NGG 2 0.422 Tier II
14 NC_000023.11 X 40645551 + CXorf38 NNNTGCAGTGGAAAAAAATG NGG 2 0.4196 Tier II
15 NC_000005.10 5 151905305 - GLRA1 NNNTGTAGAGGAAAAAAACC NGG 2 0.398 Tier II
16 NC_000005.10 5 68284031 + PIK3R1 NNNTGCAGTGGAAAAAAAAG NGG 2 0.3896 Tier II
17 NC_000008.11 8 68809046 + C8orf34 NNNTGAAGTGGAAAAAAACC NGG 2 0.3714 Tier II
18 NC_000023.11 X 33198260 - DMD NNNTCTAGTGGAAAAAAATG NGG 2 0.3626 Tier II
19 NC_000011.10 11 27432884 - LGR4 NNNTGTAGTGGGAAAAAAAG NGG 2 0.3095 Tier II
20 NC_000012.12 12 125281439 + TMEM132B NNNTGTGGTGGAAAAAAACC NGG 2 0.3025 Tier II
21 NC_000003.12 3 46252869 - CCR3 NNNTGTAGTGGAAAGAAAAG NGG 2 0.2786 Tier II
22 NC_000016.10 16 89699391 - SPATA2L NNNTGTAGTGGAAAAAAACA NAG 2 0.2431 Tier II
23 NC_000011.10 11 85802933 - SYTL2 NNNTGTAGTGGAAATAAACT NGG 2 0.14 Tier II
24 NC_000001.11 1 115081554 + TSPAN2 NNNTGTAGTGGATAAAAATG NGG 2 0.1385 Tier II
25 NC_000011.10 11 125302808 - PKNOX2 NNNTGTAGTGGGAAAAGACG NGG 2 0.1275 Tier II
26 NC_000003.12 3 113753531 + ATP6V1A NNNAGTAGTGGCAAAAAACG NGG 2 0.1253 Tier II
27 NC_000018.10 18 12706743 - PSMG2 NNNTGTAGTGGAAAAATACA NGG 2 0.125 Tier II
28 NC_000003.12 3 57382097 - DNAH12 NNNTGTAGTGTCAAAAAACG NGG 2 0.1012 Tier II
29 NC_000013.11 13 33395488 + STARD13 NNNTGTAGTGGAAATAAAAG NGG 2 0.0857 Tier II
30 NC_000010.11 10 101393715 + BTRC NNNAGTAGTGGAAAAACACG NGG 2 0.084 Tier II
31 NC_000003.12 3 53600415 + CACNA1D NNNTGTAGTGCAAAAAGACG NGG 2 0.0756 Tier II
32 NC_000006.12 6 7870540 - BMP6 NNNTGTAGTGGAAAAGAAGG NGG 2 0.024 Tier II
33 NC_000010.11 10 32854846 - CCDC7 NNNTGTAGTGGCAAAAAACG NTG 2 0.0103 Tier II
34 NC_000002.12 2 173198108 + MAP3K20-AS1 NNNTGTAGTGGAAAAAAACG NGG 0 1.0 Tier III
35 NC_000014.9 14 59172587 + LOC107984642 NNNTGTAATGGAAAAAAACA NGG 2 0.9375 Tier III
36 NC_000004.12 4 168184811 - LOC105377524 NNNTGTAGAGGAAAAAAACT NGG 2 0.65 Tier III
37 NC_000005.10 5 152907779 + LINC01470 NNNTGTAGTGGAAAAAAAAT NGG 2 0.3 Tier III
38 NC_000014.9 14 21953434 - TRA NNNTGTACTGGAAAAAAATG NGG 2 0.284 Tier III
39 NC_000019.10 19 53305185 - LOC107987270 NNNTGTTGTGGAAAAAAAAG NGG 2 0.1875 Tier III
40 NC_000004.12 4 49196380 - LOC101927209 NNNTGTAGTGGTAAAAAATG NGG 2 0.1538 Tier III
41 NC_000021.9 21 8793632 - LINC01666 NNNTGTAGTGGTAAAAAATG NGG 2 0.1538 Tier III
42 NC_000021.9 21 9101017 + TEKT4P2 NNNTGTAGTGGTAAAAAATG NGG 2 0.1538 Tier III
43 NT_113796.3 14 96567 + LOC105379271 NNNTGTAGTGGTAAAAAATG NGG 2 0.1538 Tier III
44 NT_113889.1 69004 + LOC100233156 NNNTGTAGTGGTAAAAAATG NGG 2 0.1538 Tier III
45 NC_000003.12 3 181691030 + SOX2-OT NNNTGTAGTGGAAACAAAAG NGG 2 0.1169 Tier III
46 NC_000018.10 18 73690829 - LOC105372190 NNNTGTAGTGGAAAAAAACG NGC 1 0.0222 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 72378716 + Map3k20 NNNTGTAGTGGAAAAAAACG NGG 0 1.0 Tier I
2 NC_000073.6 7 49270062 + Nav2 NNNTGGAGTGAAAAAAAACG NGG 2 0.5714 Tier II
3 NC_000077.6 11 96280918 - Hoxb8 NNNTGGAGTGGAAAAAAACA NGG 2 0.5357 Tier II
4 NC_000081.6 15 25969781 - Retreg1 NNNTGTTGAGGAAAAAAACG NGG 2 0.4062 Tier II
5 NC_000072.6 6 144699509 - Sox5 NNNTGTAGTGGAAAAAAAAT NGG 2 0.3 Tier II
6 NC_000068.7 2 68023007 - B3galt1 NNNTTTAGTGGAAACAAACG NGG 2 0.0818 Tier II
7 NC_000074.6 8 14593015 + Dlgap2 NNNTGTAGTGGAAAAAATGG NGG 2 0.0625 Tier II
8 NC_000083.6 17 90840133 - Nrxn1 NNNTGTAGTGGAAAAATATG NGG 2 0.0615 Tier II
9 NC_000069.6 3 96854916 + Pdzk1 NNNTGTAGTGGAAAAATAGG NGG 2 0.0167 Tier II
10 NC_000077.6 11 33820153 + Kcnip1 NNNTGTAGTGGAAAAAAATG NGC 2 0.0103 Tier II
11 NC_000084.6 18 43638086 - Jakmip2 NNNTGTAGTGGAAAACAAAG NGG 2 0.0 Tier II
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Other clones with same target sequence:

(none)