Construct: sgRNA BRDN0001146541

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTTCAGGAGAGAATACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JAK2 (3717)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75726

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 5080247 + JAK2 NNNTCAGGAGAGAATACCAT NGG 0 1.0 Tier I
2 NC_000018.10 18 26038555 + SS18 NNNTCAGGAGAGATTACCAT NAG 2 0.1383 Tier I
3 NC_000013.11 13 45027879 + GPALPP1 NNNTCAAGAGAGAATACCAT NTG 2 0.039 Tier I
4 NC_000009.12 9 5080247 + INSL6 NNNTCAGGAGAGAATACCAT NGG 0 1.0 Tier II
5 NC_000002.12 2 196140460 - STK17B NNNCCAAGAGAGAATACCAT NGG 2 0.6471 Tier II
6 NC_000004.12 4 80029183 - ANTXR2 NNNTTAGAAGAGAATACCAT NGG 2 0.6364 Tier II
7 NC_000010.11 10 49066127 - VSTM4 NNNTTAGGAAAGAATACCAT NGG 2 0.5939 Tier II
8 NC_000008.11 8 132202369 - KCNQ3 NNNTCAAGAGAGAATACCAA NGG 2 0.5625 Tier II
9 NC_000014.9 14 29771171 + PRKD1 NNNTCAGGAGAGAATACCAA NGG 1 0.5625 Tier II
10 NC_000007.14 7 30673076 + CRHR2 NNNTCAGAAGAGAATACCTT NGG 2 0.5385 Tier II
11 NC_000014.9 14 88857531 + TTC8 NNNACAGGAGAAAATACCAT NGG 2 0.4444 Tier II
12 NC_000005.10 5 120514376 - PRR16 NNNTCAGGAGAGAATACTAA NGG 2 0.3616 Tier II
13 NC_000002.12 2 43718017 + PLEKHH2 NNNGAAGGAGAGAATACCAT NGG 2 0.3571 Tier II
14 NC_000007.14 7 117655105 - CFTR NNNTCAGGAGAGAATACAAA NGG 2 0.3029 Tier II
15 NC_000012.12 12 43390363 - ADAMTS20 NNNTCAGGAGAGAATACAAA NGG 2 0.3029 Tier II
16 NC_000006.12 6 39068926 - GLP1R NNNTCAGGAGACAATACCAA NGG 2 0.2978 Tier II
17 NC_000008.11 8 103024423 + ATP6V1C1 NNNTCACGAGATAATACCAT NGG 2 0.2644 Tier II
18 NC_000004.12 4 93227219 - GRID2 NNNTCAGGAGAGAATATCAA NGG 2 0.2625 Tier II
19 NC_000008.11 8 4209028 - CSMD1 NNNACAGGAGAGAATACAAT NGG 2 0.2564 Tier II
20 NC_000011.10 11 99360786 - CNTN5 NNNTCAGGAGAGAACACCAA NGG 2 0.1534 Tier II
21 NC_000010.11 10 116079848 + GFRA1 NNNTCAGCAGAGAATACCAG NGG 2 0.1086 Tier II
22 NC_000008.11 8 55531991 - XKR4 NNNTCAGGAGAGAATGCAAT NGG 2 0.1036 Tier II
23 NC_000007.14 7 33416317 - BBS9 NNNTCAGGAGACAATACCAG NGG 2 0.0934 Tier II
24 NC_000014.9 14 51243184 + TMX1 NNNTCAGGAGATAATACCAG NGG 2 0.0679 Tier II
25 NC_000011.10 11 73333304 + ARHGEF17 NNNTCAGGAGAGTATACCCT NGG 2 0.0621 Tier II
26 NC_000013.11 13 75705381 - LMO7 NNNTCAGGAGAGAATACCTC NGG 2 0.049 Tier II
27 NC_000010.11 10 89638460 - PANK1 NNNTCAGGAGAGAAGACCAA NGG 2 0.0281 Tier II
28 NC_000010.11 10 113575417 - HABP2 NNNTCAGGAGAGAAGACCAA NGG 2 0.0281 Tier II
29 NC_000011.10 11 46362877 - DGKZ NNNTCAGGAGACAAGACCAT NGG 2 0.0265 Tier II
30 NC_000005.10 5 13997137 - DNAH5 NNNTCAGGAGAGAACACCAT NGA 2 0.0189 Tier II
31 NC_000014.9 14 52524140 + TXNDC16 NNNTCAGGAGAGAACAGCAT NGG 2 0.016 Tier II
32 NC_000001.11 1 16923574 - CROCC NNNTCAGGAGAGCATAGCAT NGG 2 0.0124 Tier II
33 NC_000019.10 19 50205035 + MYH14 NNNTCAGGACAGAATACCAT NGT 2 0.0065 Tier II
34 NC_000021.9 21 25888095 - APP NNNTCAGGACAGAATACCAT NGT 2 0.0065 Tier II
35 NC_000014.9 14 51964141 + GNG2 NNNTCAGGAGAGAATTCCAT NTG 2 0.0 Tier II
36 NC_000023.11 X 150863848 + CD99L2 NNNTCAGGAGAGAATTCCAT NTG 2 0.0 Tier II
37 NC_000008.11 8 39400472 - ADAM5 NNNTCTGGAGAGAATACCAA NGG 2 0.4018 Tier III
38 NC_000008.11 8 20775013 - LOC105379315 NNNTCAGGATAGAATATCAT NGG 2 0.3792 Tier III
39 NC_000023.11 X 103473292 + LL0XNC01-250H12.3 NNNTCAGGAGAGAATACTAA NGG 2 0.3616 Tier III
40 NC_000008.11 8 82115286 - LINC02839 NNNTCAGGAGAGAAAACCAA NGG 2 0.3257 Tier III
41 NC_000002.12 2 111181116 - LOC124907868 NNNACAGGAGAGAATGCCAT NGG 2 0.0916 Tier III
42 NC_000011.10 11 73333304 + LOC124902710 NNNTCAGGAGAGTATACCCT NGG 2 0.0621 Tier III
43 NC_000003.12 3 142972007 + PAQR9-AS1 NNNTCAGGAGAGACTAGCAT NGG 2 0.0126 Tier III
44 NC_000014.9 14 96669575 - LOC105370645 NNNTCAGGAGAGAATCTCAT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 29298311 + Jak2 NNNTCAGGAGAGAATACCAT NGG 0 1.0 Tier I
2 NC_000080.6 14 76090653 - Gpalpp1 NNNCCAGGAGAGAATACCAT NTG 2 0.0252 Tier I
3 NC_000067.6 1 110947107 + Cdh19 NNNTCAGGAAAGAATACAAT NGG 2 0.5026 Tier II
4 NC_000083.6 17 89144102 - Fshr NNNTCAGGTGAGATTACCAT NGG 2 0.32 Tier II
5 NC_000077.6 11 43163449 + Atp10b NNNTCAGGAGAGAATACATT NGG 2 0.2899 Tier II
6 NC_000074.6 8 63020456 + Spock3 NNNCCAGGAGAGAATACCAC NGG 2 0.0588 Tier II
7 NC_000071.6 5 139455890 - 3110082I17Rik NNNTCAGGTGAGAAGACCAT NGG 2 0.03 Tier II
8 NC_000086.7 X 42874191 - Tenm1 NNNTCAGGAGAGAATACTAT NTG 2 0.025 Tier II
9 NC_000082.6 16 96498789 + Pcp4 NNNTCAGGAGAGAAGACCAT NGT 2 0.0008 Tier II
10 NC_000069.6 3 126634124 - Camk2d NNNTCAGGAAAGAATCCCAT NGG 2 0.0 Tier II
11 NC_000071.6 5 45029334 + D5Ertd615e NNNTCAGGAGAGAGTACCAT NGG 1 0.4667 Tier III
12 NC_000071.6 5 111937927 - Gm36794 NNNTCAGAACAGAATACCAT NGG 2 0.4 Tier III
13 NC_000085.6 19 19247735 + Gm50212 NNNTCAGTAGAGACTACCAT NGG 2 0.1339 Tier III
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Other clones with same target sequence:

(none)