Construct: sgRNA BRDN0001146542
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATACTTGTAACTACAAAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK6 (5597)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76399
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
52064176 |
+ |
MAPK6 |
NNNCTTGTAACTACAAAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
122454397 |
+ |
SERINC1 |
NNNTTTGTAACTACAAAACA |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000008.11 |
8 |
109470299 |
+ |
PKHD1L1 |
NNNCTTGTAACTGCAAAACG |
NGG |
1 |
0.6522 |
Tier II |
| 4 |
NC_000010.11 |
10 |
34251493 |
- |
PARD3 |
NNNCTTGTATCTGCAAAACG |
NGG |
2 |
0.5754 |
Tier II |
| 5 |
NC_000013.11 |
13 |
42069753 |
- |
DGKH |
NNNCTTGTAATTACAAAACG |
NGG |
1 |
0.3077 |
Tier II |
| 6 |
NC_000012.12 |
12 |
57300570 |
- |
R3HDM2 |
NNNCTTGTAAGTACAAAACC |
NGG |
2 |
0.1071 |
Tier II |
| 7 |
NC_000002.12 |
2 |
152406064 |
+ |
FMNL2 |
NNNCTTGTGACTACAAAAGG |
NGG |
2 |
0.0833 |
Tier II |
| 8 |
NC_000001.11 |
1 |
237485481 |
+ |
RYR2 |
NNNCTTGTTACTACAAAAGG |
NGG |
2 |
0.075 |
Tier II |
| 9 |
NC_000008.11 |
8 |
46973293 |
- |
MAPK6P4 |
NNNCTTGTAACTACAAAACG |
NGG |
0 |
1.0 |
Tier III |
| 10 |
NC_000015.10 |
15 |
52064176 |
+ |
LOC112268150 |
NNNCTTGTAACTACAAAACG |
NGG |
0 |
1.0 |
Tier III |
| 11 |
NC_000008.11 |
8 |
109470299 |
+ |
MAPK6P5 |
NNNCTTGTAACTGCAAAACG |
NGG |
1 |
0.6522 |
Tier III |
| 12 |
NC_000010.11 |
10 |
49772087 |
+ |
MAPK6P6 |
NNNCTTGTAACTGCAAAACG |
NGG |
1 |
0.6522 |
Tier III |
| 13 |
NC_000012.12 |
12 |
93248087 |
- |
LOC643339 |
NNNCTTGTAAATATAAAACG |
NGG |
2 |
0.55 |
Tier III |
| 14 |
NC_000008.11 |
8 |
53539474 |
+ |
MAPK6P1 |
NNNTTTGTAACTACAAAATG |
NGG |
2 |
0.3692 |
Tier III |
| 15 |
NC_000013.11 |
13 |
42069753 |
- |
MAPK6P3 |
NNNCTTGTAATTACAAAACG |
NGG |
1 |
0.3077 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
27804665 |
+ |
Grm8 |
NNNCTTGAAACTACAAAAAG |
NGG |
2 |
0.3429 |
Tier II |
| 2 |
NC_000077.6 |
11 |
11882228 |
+ |
Ddc |
NNNCTTCTAACTACAAAAGG |
NGG |
2 |
0.0859 |
Tier II |
| 3 |
NC_000086.7 |
X |
113151341 |
+ |
Chm |
NNNCTTGTAACTACAAAACA |
NGA |
2 |
0.0651 |
Tier II |
| 4 |
NC_000071.6 |
5 |
20854601 |
+ |
Phtf2 |
NNNCTTGTAAGTACAAAACG |
NTG |
2 |
0.0097 |
Tier II |
| 5 |
NC_000078.6 |
12 |
80673799 |
- |
Slc39a9 |
NNNCTTGTAACTACACAACT |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000077.6 |
11 |
11882228 |
+ |
1700042O10Rik |
NNNCTTCTAACTACAAAAGG |
NGG |
2 |
0.0859 |
Tier III |
Other clones with same target sequence:
(none)
