Construct: sgRNA BRDN0001146545
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGCGGCTGCCGGAACACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3CD (5293)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76165
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
9716519 |
- |
PIK3CD |
NNNCGGCTGCCGGAACACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
130431418 |
+ |
HMCN2 |
NNNCGCCTGCCGCAACACTG |
NGG |
2 |
0.2871 |
Tier I |
| 3 |
NC_000007.14 |
7 |
751990 |
+ |
DNAAF5 |
NNNCAGCTGCCGAAACACTG |
NGG |
2 |
0.8 |
Tier II |
| 4 |
NC_000002.12 |
2 |
153818587 |
+ |
GALNT13 |
NNNCCCCTGCCGGAACACTG |
NGG |
2 |
0.5357 |
Tier II |
| 5 |
NC_000002.12 |
2 |
120818131 |
+ |
GLI2 |
NNNCAGCTGCCTGAACACTG |
NGG |
2 |
0.3333 |
Tier II |
| 6 |
NC_000001.11 |
1 |
184562192 |
- |
C1orf21 |
NNNCAGCTGCTGGAACACTG |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000002.12 |
2 |
43759529 |
+ |
PLEKHH2 |
NNNCTGCTGCAGGAACACTG |
NGG |
2 |
0.225 |
Tier II |
| 8 |
NC_000008.11 |
8 |
22914677 |
+ |
PEBP4 |
NNNCGCCTGCTGGAACACTG |
NGG |
2 |
0.2098 |
Tier II |
| 9 |
NC_000001.11 |
1 |
16155050 |
+ |
EPHA2 |
NNNCGGCTCCGGGAACACTG |
NGG |
2 |
0.1346 |
Tier II |
| 10 |
NC_000018.10 |
18 |
13390293 |
- |
LDLRAD4 |
NNNCGGCTCCCGGCACACTG |
NGG |
2 |
0.1154 |
Tier II |
| 11 |
NC_000001.11 |
1 |
182069327 |
+ |
ZNF648 |
NNNCGGCTGCCTGAACACAG |
NGG |
2 |
0.1099 |
Tier II |
| 12 |
NC_000020.11 |
20 |
63541807 |
- |
SRMS |
NNNCGGCTGCCGGGTCACTG |
NGG |
2 |
0.0933 |
Tier II |
| 13 |
NC_000009.12 |
9 |
130431418 |
+ |
LOC107987134 |
NNNCGCCTGCCGCAACACTG |
NGG |
2 |
0.2871 |
Tier III |
| 14 |
NC_000006.12 |
6 |
4563353 |
- |
LOC105374894 |
NNNCTGCTGCCCGAACACTG |
NGG |
2 |
0.1588 |
Tier III |
| 15 |
NC_000011.10 |
11 |
71851473 |
- |
XNDC1N-ZNF705EP-ALG1L9P |
NNNCTGCTGCCTGAACACTG |
NGG |
2 |
0.1154 |
Tier III |
| 16 |
NC_000002.12 |
2 |
8599611 |
- |
LOC105373411 |
NNNCGGCGGCCGGAAGACTG |
NGG |
2 |
0.1128 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
149659072 |
+ |
Pik3cd |
NNNAGGCTGCCGGAACACTG |
NGG |
1 |
0.8421 |
Tier I |
| 2 |
NC_000070.6 |
4 |
126507319 |
+ |
Ago4 |
NNNAGGCTGCTGGAACACTG |
NGG |
2 |
0.2591 |
Tier I |
| 3 |
NC_000083.6 |
17 |
27973104 |
- |
Anks1 |
NNNCGGCTACCGGAACACTT |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000084.6 |
18 |
7693218 |
+ |
Gm18763 |
NNNCGGCTGCCGGAGCTCTG |
NGG |
2 |
0.0867 |
Tier III |
Other clones with same target sequence:
(none)
